DOI: 10.1111/j.1751-1097.1987.tb07881.x. ACTION SPECTRA FOR THE INDUCTION OF PYRIMIDINE(6‐4)PYRIMIDONE PHOTOPRODUCTS AND CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL HUMAN SKIN FIBROBLASTS

ACTION SPECTRA FOR THE INDUCTION OF PYRIMIDINE(6‐4)PYRIMIDONE PHOTOPRODUCTS AND CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL HUMAN SKIN FIBROBLASTS

10.1111/j.1751-1097.1987.tb07881.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract. Normal human skin fibroblasts were exposed to 265–313‐nm monochromatic UV wavelengths and the yield of pyrimidine(6‐4)pyrimidone photoproducts [(6‐4) photoproducts] and cyclobutane pyrimidine dimers (dimers) measured by radioimmunoassay. The action spectra for the induction of these two types of DNA damage were very similar for wavelengths from 254 to 302 nm. However, the action spectrum value for (6‐4) photoproduct production was less than half the value obtained for dimer induction by 313‐nm UV. Cells were also exposed to the UV produced by a broad‐spectrum fluorescent sunlamp (280–360 nm, peak at 313 nm) under conditions in which various wavelength components were removed from the spectrum. For exposures in which the (6‐4) photoproducts and dimers were induced primarily by wavelengths shorter than 310 nm, their rates of induction, relative to 254‐nm‐irradiated cells, were similar. However, the level of (6‐4) photoproduct production was about three‐fold lower than dimer induction for sunlamp irradiations in which the 315–330‐nm component of this source was primarily responsible for the formation of this damage. These results are consistent with the conclusion that for treatments in which (6‐4) photoproducts are produced by wavelengths in the 310–320‐nm range, which represent the region of absorption peaks for these photoproducts, both the induction and photolysis of (6‐4) photoproducts occur simultaneously during irradiation.

Bibliography

Rosenstein, B. S., & Mitchell, D. L. (1987). ACTION SPECTRA FOR THE INDUCTION OF PYRIMIDINE(6â4)PYRIMIDONE PHOTOPRODUCTS AND CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL HUMAN SKIN FIBROBLASTS. Photochemistry and Photobiology, 45(S1), 775â780. Portico.

Authors 2

Barry S. Rosenstein (first)
David L. Mitchell (additional)

References 26 Referenced 152

10.1038/298189a0
10.1080/09553008614551041
10.1016/0167-8817(83)90004-4
10.1016/S0021-9258(18)33480-X / J. Biol. Chem. / Alkaline lability of fluorescent photoproducts produced in ultraviolet‐light‐irradiated DNA by Franklin W. A. (1982)
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{'key': 'e_1_2_2_15_1', 'article-title': 'The importance of (6–4) photoproducts and cyclobutane dimers for split‐dose recovery in UV‐irradiated normal and hypersensitive rodent cells', 'author': 'Mitchell D. L.', 'year': '1987', 'journal-title': 'Mutat. Res.'} / Mutat. Res. / The importance of (6–4) photoproducts and cyclobutane dimers for split‐dose recovery in UV‐irradiated normal and hypersensitive rodent cells by Mitchell D. L. (1987)
10.1016/0167-4781(82)90089-6
10.1111/j.1751-1097.1970.tb06018.x
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10.1111/j.1751-1097.1965.tb07916.x
10.1111/j.1751-1097.1984.tb04577.x
10.1111/j.1751-1097.1983.tb08365.x
10.1016/0027-5107(86)90095-3
10.1126/science.156.3777.955
10.1126/science.160.3824.186
10.1016/0006-291X(67)90519-0
10.1016/0022-2836(84)90121-9

Dates

Type	When
Created	17 years, 8 months ago (Jan. 2, 2008, 4:21 p.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 5:15 a.m.)
Indexed	3 months ago (June 5, 2025, 8:44 a.m.)
Issued	38 years, 4 months ago (May 1, 1987)
Published	38 years, 4 months ago (May 1, 1987)
Published Online	17 years, 8 months ago (Jan. 2, 2008)
Published Print	38 years, 4 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Rosenstein_1987, title={ACTION SPECTRA FOR THE INDUCTION OF PYRIMIDINE(6‐4)PYRIMIDONE PHOTOPRODUCTS AND CYCLOBUTANE PYRIMIDINE DIMERS IN NORMAL HUMAN SKIN FIBROBLASTS}, volume={45}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1987.tb07881.x}, DOI={10.1111/j.1751-1097.1987.tb07881.x}, number={S1}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Rosenstein, Barry S. and Mitchell, David L.}, year={1987}, month=may, pages={775–780} }

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