DOI: 10.1111/j.1751-1097.1983.tb03863.x. CHLOROPHYLL<i>b</i>: AN INTEGRAL COMPONENT OF PHOTOSYSTEM I OF HIGHER PLANT CHLOROPLASTS

CHLOROPHYLLb: AN INTEGRAL COMPONENT OF PHOTOSYSTEM I OF HIGHER PLANT CHLOROPLASTS

10.1111/j.1751-1097.1983.tb03863.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract—An undissociated photosystem I complex may be isolated from spinach thylakoids by mild gel electrophoresis (CP1a) or Triton X‐100. CP1a has a Chla/bratio of 11 and a Chl/P700 ratio of 120. while the Triton X‐100 PS I complex (Chla/bratio of 5.9) has a larger antenna unit size (Chl/P700 ratio of 180). None of the Chla/b‐proteins of the main light‐harvesting complex (apoproteins of 30–27 kD) are present in CP1a, and they account for less than 10% of the total chlorophyll in the Triton X‐100 PS I complex. Instead, these PS I complexes have specific, but as yet little characterized, Chia/b‐proteins (apoproteins in the 26–21 kD range). With both PS I complexes, Chibtransfers light excitation to the 735 nm low temperature fluorescence band characteristic of photosystem I. We suggest that Chibis an integral but minor component of photosystem I.

Bibliography

Anderson, J. M., Brown, J. S., Lam, E., & Malkin, R. (1983). CHLOROPHYLLb: AN INTEGRAL COMPONENT OF PHOTOSYSTEM I OF HIGHER PLANT CHLOROPLASTS. Photochemistry and Photobiology, 38(2), 205â210. Portico.

Authors 4

Jan M. Anderson (first)
Jeanette S. Brown (additional)
Eric Lam (additional)
Richard Malkin (additional)

References 27 Referenced 60

10.1016/0005-2728(80)90225-X
{'key': 'e_1_2_2_2_1', 'first-page': '301', 'volume-title': 'Photosynthesis', 'author': 'Anderson J. M.', 'year': '1981'} / Photosynthesis by Anderson J. M. (1981)
10.1016/0014-5793(78)80760-1
10.1016/0005-2728(80)90078-X
10.1016/0014-5793(79)81088-1
10.1104/pp.24.1.1
10.1016/0005-2728(71)90248-9
{'key': 'e_1_2_2_8_1', 'first-page': '35', 'volume': '8', 'author': 'Boardman N. K.', 'year': '1978', 'journal-title': 'Current Topics in Bioenergetics'} / Current Topics in Bioenergetics by Boardman N. K. (1978)
{'key': 'e_1_2_2_9_1', 'first-page': '505', 'volume-title': 'Photosynthesis III', 'author': 'Borchert M. T.', 'year': '1981'} / Photosynthesis III by Borchert M. T. (1981)
10.1016/S0044-328X(81)80099-2
10.1016/B978-0-12-282608-5.50009-6
{'key': 'e_1_2_2_12_1', 'first-page': '209', 'volume': '76', 'author': 'Brown J. S.', 'year': '1977', 'journal-title': 'Carnegie Institution of Washington Year Book'} / Carnegie Institution of Washington Year Book by Brown J. S. (1977)
10.1007/BF00030046
10.1016/0003-9861(78)90031-0
10.1104/pp.66.3.428
{'key': 'e_1_2_2_16_1', 'first-page': '50', 'volume-title': 'In Electron Transport and Photophosphorylation', 'author': 'Cox R. P.', 'year': '1982'} / In Electron Transport and Photophosphorylation by Cox R. P. (1982)
{'key': 'e_1_2_2_17_1', 'first-page': '482', 'volume': '245', 'author': 'Machold O.', 'year': '1979', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Machold O. (1979)
10.1104/pp.65.5.814
10.1104/pp.65.5.823
{'key': 'e_1_2_2_20_1', 'first-page': '557', 'volume-title': 'Photosynthesis', 'author': 'Mullet J. E.', 'year': '1981'} / Photosynthesis by Mullet J. E. (1981)
{'key': 'e_1_2_2_21_1', 'first-page': '235', 'volume-title': 'Chloroplast Development', 'author': 'Remy R.', 'year': '1978'} / Chloroplast Development by Remy R. (1978)
10.1111/j.1432-1033.1980.tb06035.x / Eur. J. Biochem. by Ryrie I. J. (1980)
10.1016/0014-5793(79)81087-X
10.1111/j.1751-1097.1982.tb02636.x
{'key': 'e_1_2_2_25_1', 'first-page': '27', 'volume-title': 'In Photosynthesis in Relation to Model Systems', 'author': 'Thornber J. P.', 'year': '1979'} / In Photosynthesis in Relation to Model Systems by Thornber J. P. (1979)
10.1111/j.1751-1097.1979.tb07840.x
10.1016/0005-2728(82)90149-9

Dates

Type	When
Created	17 years, 8 months ago (Jan. 2, 2008, 5:08 p.m.)
Deposited	1 year, 6 months ago (Feb. 20, 2024, 6:43 p.m.)
Indexed	1 month ago (Aug. 6, 2025, 8:34 a.m.)
Issued	42 years, 1 month ago (Aug. 1, 1983)
Published	42 years, 1 month ago (Aug. 1, 1983)
Published Online	17 years, 8 months ago (Jan. 2, 2008)
Published Print	42 years, 1 month ago (Aug. 1, 1983)

Funders 0

None

BibTeX

@article{Anderson_1983, title={CHLOROPHYLLb: AN INTEGRAL COMPONENT OF PHOTOSYSTEM I OF HIGHER PLANT CHLOROPLASTS}, volume={38}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1983.tb03863.x}, DOI={10.1111/j.1751-1097.1983.tb03863.x}, number={2}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Anderson, Jan M. and Brown, Jeanette S. and Lam, Eric and Malkin, Richard}, year={1983}, month=aug, pages={205–210} }

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