DOI: 10.1111/j.1751-1097.1981.tb05465.x. P<sub>588</sub>, A SECOND RETINAL‐CONTAINING PIGMENT IN <i>HALOBACTERIUM HALOBIUM</i>

P588, A SECOND RETINAL‐CONTAINING PIGMENT IN HALOBACTERIUM HALOBIUM

10.1111/j.1751-1097.1981.tb05465.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— Halobacterium mutant strains with defects in the biosynthesis of various pigments have been isolated. One of these strains, mutant ET‐15, is incapable of producing the light‐driven proton pump bacteriorhodopsin and the carotenoid bacterioruberin. However, ET‐15 synthesizes another photochemically active, retinal‐containing pigment, P588, which mediates light‐induced proton uptake enhanced by uncouplers. P588 and bacteriorhodopsin are simultaneously present in wild‐type cells grown under normal conditions; however, they can be distinguished by the following criteria. They can be separated by independent mutational events. Proton ionophores such as FCCP diminish bacteriorhodopsin‐driven proton translocation but enhance P588‐mediated proton flows. We define here proton translocations which can be diminished by the addition of uncouplers (e.g. FCCP) as ‘active’, others which can be enhanced by FCCP as ‘passive’. The kinetic and spectral properties of the photocycle intermediates of bacteriorhodopsin and P588 are different. The action spectrum for photocycling of P588 is red‐shifted with respect to that for bacteriorhodopsin. A comparison of action spectra for proton translocations with that for photocycling links pigment P588 and light‐driven, passive proton uptake by ET‐15 envelope vesicles. When chemically bleached P588 was regenerated with all‐trans‐retinal, both photochemical activity and light‐induced proton inflow were restored to equal extents. This identifies P588 with the energizer for the passive proton flows.

Bibliography

Weber, H. J., & Bogomolni, R. A. (1981). P588, A SECOND RETINALâCONTAINING PIGMENT IN HALOBACTERIUM HALOBIUM. Photochemistry and Photobiology, 33(4), 601â608. Portico.

Authors 2

H. J. Weber (first)
R. A. Bogomolni (additional)

References 24 Referenced 53

10.1016/0005-2728(76)90114-6
10.1021/bi00551a024
{'key': 'e_1_2_2_4_1', 'first-page': '1846', 'volume': '39', 'author': 'Bogomolni R. A.', 'year': '1980', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Bogomolni R. A. (1980)
10.1073/pnas.71.4.1234
10.1016/0005-2736(77)90276-0
10.1016/S0021-9258(19)86620-6 / J. Biol. Chem. by Green R. V. (1979)
{'key': 'e_1_2_2_8_1', 'first-page': '146', 'volume': '146', 'author': 'Heefner D. L.', 'year': '1979', 'journal-title': 'Proc. Am. Soc. Microbiol.'} / Proc. Am. Soc. Microbiol. by Heefner D. L. (1979)
10.1016/0006-291X(75)90154-0
{'key': 'e_1_2_2_10_1', 'author': 'Kushwaha S. C.', 'year': '1980', 'journal-title': 'Can. J. Microbiol.'} / Can. J. Microbiol. by Kushwaha S. C. (1980)
10.1021/bi00666a010
10.1016/S0021-9258(19)86290-7
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{'key': 'e_1_2_2_15_1', 'first-page': '1806', 'volume': '36', 'author': 'Lozier R. H.', 'year': '1977', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Lozier R. H. (1977)
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10.1002/9780470720134.ch9
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{'key': 'e_1_2_2_22_1', 'first-page': '667', 'volume-title': 'Methods in Enzymology', 'author': 'Oesterhelt D.', 'year': '1974'} / Methods in Enzymology by Oesterhelt D. (1974)
10.1016/S0021-9258(19)43080-9
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Weber H. J.andI.Probst(1980)J. Biol. Chem.(Submitted).

Dates

Type	When
Created	17 years, 8 months ago (Jan. 2, 2008, 5:25 p.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 1:27 p.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 8:55 a.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Online	17 years, 8 months ago (Jan. 2, 2008)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Weber_1981, title={P588, A SECOND RETINAL‐CONTAINING PIGMENT IN HALOBACTERIUM HALOBIUM}, volume={33}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1981.tb05465.x}, DOI={10.1111/j.1751-1097.1981.tb05465.x}, number={4}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Weber, H. J. and Bogomolni, R. A.}, year={1981}, month=apr, pages={601–608} }

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