DOI: 10.1111/j.1751-1097.1979.tb07187.x. CAROTENOID TRIPLET STATE AND CHLOROPHYLL FLUORESCENCE QUENCHING IN CHLOROPLASTS AND SUBCHLOROPLAST PARTICLES

CAROTENOID TRIPLET STATE AND CHLOROPHYLL FLUORESCENCE QUENCHING IN CHLOROPLASTS AND SUBCHLOROPLAST PARTICLES

10.1111/j.1751-1097.1979.tb07187.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— Absorption changes attributed to the triplet state of carotenoids and to primary electron donors (P‐700. P‐680): and fluorescence quenching at several wavelengths have been measured with a single apparatus. following flash excitation with a dye or a ruby laser. Spinach chloroplasts as well as subchloroplast particles enriched in Photosystem‐1 (F1), Photosystem‐2 (F1) or the light‐harvesting Chl a/h (FIII) have been examined at temperatures varying between 5 and 294 K.The triplet state of carotenoids has been identified on the basis of its difference spectrum (having a peak at 515 nm) and decay kinetics (⋍ 7 µs at low temperature; accelerated by O2 at 294 K). It is formed in all of the materials studied. The quantum yield of carotenoid triplet formation in chloroplasts increases at low temperature, but less than the fluorescence yield.In most cases the fluorescence quenching recovers approximately with the same kinetics as the decay of the carotenoid triplets. The fluorescence recovery is, however, significantly faster for chloroplasts at 730 nm. Fluorescence quenching occurs in all types of materials. The ratio of fluorescence quenching to the concentration of carotenoid triplets varies with the material, being maximum in chloroplasts and minimum in Fm particles.We conclude that the formation of the carotenoid triplet state is not limited to a few sites in the chloroplast and that a carotenoid triplet is a quencher of chlorophyll fluorescence. A detailed comparison of carotenoid triplets and fluorescence quenching gives some information concerning the organization of the pigments in the photosynthetic apparatus.

Bibliography

Mathis, P., Butler, W. L., & Satoh, K. (1979). CAROTENOID TRIPLET STATE AND CHLOROPHYLL FLUORESCENCE QUENCHING IN CHLOROPLASTS AND SUBCHLOROPLAST PARTICLES. Photochemistry and Photobiology, 30(5), 603â614. Portico.

Authors 3

P. Mathis (first)
W. L. Butler (additional)
K. Satoh (additional)

References 38 Referenced 77

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{'key': 'e_1_2_1_3_1', 'first-page': '43', 'volume': '296', 'author': 'Bowers P. G.', 'year': '1967', 'journal-title': 'Proc. Roy. Soc.'} / Proc. Roy. Soc. by Bowers P. G. (1967)
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{'key': 'e_1_2_1_15_1', 'first-page': '353', 'volume-title': 'Studies on Microalgae and Photosynthetic Bacteria', 'author': 'Duysens L. N. M.', 'year': '1963'} / Studies on Microalgae and Photosynthetic Bacteria by Duysens L. N. M. (1963)
{'key': 'e_1_2_1_16_1', 'first-page': '1', 'volume-title': 'Proc. Third Int. Congr. Photosynth.', 'author': 'Duysens L. N. M.', 'year': '1975'} / Proc. Third Int. Congr. Photosynth. by Duysens L. N. M. (1975)
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Dates

Type	When
Created	17 years, 7 months ago (Jan. 2, 2008, 2:59 p.m.)
Deposited	1 year, 10 months ago (Oct. 6, 2023, 8:33 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 9:55 a.m.)
Issued	45 years, 9 months ago (Nov. 1, 1979)
Published	45 years, 9 months ago (Nov. 1, 1979)
Published Online	17 years, 7 months ago (Jan. 2, 2008)
Published Print	45 years, 9 months ago (Nov. 1, 1979)

Funders 0

None

BibTeX

@article{Mathis_1979, title={CAROTENOID TRIPLET STATE AND CHLOROPHYLL FLUORESCENCE QUENCHING IN CHLOROPLASTS AND SUBCHLOROPLAST PARTICLES}, volume={30}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1979.tb07187.x}, DOI={10.1111/j.1751-1097.1979.tb07187.x}, number={5}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Mathis, P. and Butler, W. L. and Satoh, K.}, year={1979}, month=nov, pages={603–614} }

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