DOI: 10.1111/j.1751-1097.1976.tb07274.x. SIMULTANEOUS MEASUREMENT OF ACTION SPECTRA FOR PHOTOREACTIONS I AND II OF PHOTOSYNTHESIS

SIMULTANEOUS MEASUREMENT OF ACTION SPECTRA FOR PHOTOREACTIONS I AND II OF PHOTOSYNTHESIS

10.1111/j.1751-1097.1976.tb07274.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— We have devised a method of obtaining simultaneous action spectra for photoreactions I and II by analysis of direct and indirect effects involved in enhancement. The method requires previous determination of the neutral wavelength which gives maximum quantum yield by virtue of equal fractions of open reaction centers (p and q) for each photoreaction. A sufficient intensity of the neutral wavelength is used as a constant background. Upon addition of a weak modulated measuring light of intensity Im and wavelength λm two amperometric signals are obtained for rate of oxygen evolution. A modulated signal (AC¯) isolates the direct effect of Im and gives action of photoreaction II as AC/Im. An increment in total rate (ΔDC) also includes an indirect effect of Im in perturbing reaction center conditions (p and q). From analysis of interaction of the two photoreactions, action for photoreaction I can be estimated as (2 ΔDC‐AC)/Im. The method is applicable to whole cells, properly scales the two action spectra to each other, and removes contribution of the State 1‐State 2 phenomena. Action spectra were obtained for Chlorella.

Bibliography

Wang, R. T., & Myers, J. (1976). SIMULTANEOUS MEASUREMENT OF ACTION SPECTRA FOR PHOTOREACTIONS I AND II OF PHOTOSYNTHESIS. Photochemistry and Photobiology, 23(6), 411â414. Portico.

Authors 2

Richard T. Wang (first)
Jack Myers (additional)

References 10 Referenced 18

{'key': 'e_1_2_1_2_1', 'first-page': '1110', 'volume-title': 'Progress in Photosynthesis Research', 'author': 'Delrieu M. J.', 'year': '1969'} / Progress in Photosynthesis Research by Delrieu M. J. (1969)
10.1111/j.1399-3054.1974.tb03680.x
10.1016/0003-9861(63)90547-2
10.1016/0005-2728(68)90191-6
Myers J.(1963) InPhotosynthetic Mechanisms of Green Plants pp.301–317. National Academy of Sciences—National Research Council Publication No. 1145 Washington D.C.
10.1104/pp.48.3.282
Ried A.(1971) 2nd Internat. Congr. Photosynthesis Research Stresa Italy pp.763–772W. Junk The Hague. (10.1007/978-94-010-2935-3_79)
10.1515/znb-1965-1113 / Z. Naturforsch. by Rumberg B. (1965)
10.1104/pp.40.1.7
{'key': 'e_1_2_1_11_1', 'volume': '23', 'author': 'Wang R. T.', 'year': '1976', 'journal-title': 'Photochem. Photobiol.'} / Photochem. Photobiol. by Wang R. T. (1976)

Dates

Type	When
Created	17 years, 8 months ago (Jan. 2, 2008, 6:53 p.m.)
Deposited	1 year, 10 months ago (Oct. 8, 2023, 11:37 a.m.)
Indexed	1 year, 9 months ago (Nov. 6, 2023, 10:02 p.m.)
Issued	49 years, 3 months ago (June 1, 1976)
Published	49 years, 3 months ago (June 1, 1976)
Published Online	17 years, 8 months ago (Jan. 2, 2008)
Published Print	49 years, 3 months ago (June 1, 1976)

Funders 0

None

BibTeX

@article{Wang_1976, title={SIMULTANEOUS MEASUREMENT OF ACTION SPECTRA FOR PHOTOREACTIONS I AND II OF PHOTOSYNTHESIS}, volume={23}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1976.tb07274.x}, DOI={10.1111/j.1751-1097.1976.tb07274.x}, number={6}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Wang, Richard T. and Myers, Jack}, year={1976}, month=jun, pages={411–414} }

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