DOI: 10.1111/j.1751-1097.1973.tb06415.x. ON THE FORMATION OF PHOTOSYNTHETIC MEMBRANES IN BEAN PLANTS*

ON THE FORMATION OF PHOTOSYNTHETIC MEMBRANES IN BEAN PLANTS*

10.1111/j.1751-1097.1973.tb06415.x

Crossref journal-article

Wiley

Photochemistry and Photobiology (311)

Abstract

Abstract— The formation of lamellar chlorophyll‐protein complexes I and II, solubilized by sodium dodecyl sulfate, was studied by hydroxylapatite column chromatography during greening of etiolated Phaseohis vulgaris leaves.The protein moiety of both complexes preexists in the prolamellar body of etiolated tissue. The complex II to complex I protein ratio is of the order of 0.5. During greening in intermittent illumination the ‘proto’‐chloroplast is agranal, and contains ‘primary’ thylakoids and chlorophyll a (Chl a). At this stage the complex II to complex I protein ratio increases only slightly. Further greening of the plant tissue in continuous illumination results in grana, Chi b (chlorophyll b) and more Chl a formation. The complex II to complex I protein ratio in unfractionated thylakoids is now of the order of 2.5, while in grana it is of the order of 4.0.The binding of chlorophyll formed during greening to the protein moiety of the two complexes is found to be selective. The Chi a selectively formed under intermittent illumination is more strongly bound to the complex I protein. The Chi b and Chl a formed in continuous illunination are found bound to both complex I and complex II proteins.Analysis by hydroxylapatite column chromatography of subchloroplast fractions obtained by different fractionation procedures have shown that these two chlorophyll‐protein complexes are most probably derived from the PSI (photosystem I) and PSII (photosystem II) particles of the photosynthetic membrane. These findings suggest that PSI units are assembled ahead of PSII units. Moreover, they indicate that the complex I protein is the main protein component in the prolamellar body membranes, the ‘primary’ thylakoids. and the stroma lamellae, while in the grana membranes the major protein is the complex II protein. Finally our results show that formation of the photosynthetic membranes is a multi‐step process.

Bibliography

ArgyroudiâAkoyunoglou, J. H., & Akoyunoglou, G. (1973). ON THE FORMATION OF PHOTOSYNTHETIC MEMBRANES IN BEAN PLANTS*. Photochemistry and Photobiology, 18(3), 219â228. Portico.

Authors 2

J. H. Argyroudi‐Akoyunoglou (first)
G. Akoyunoglou (additional)

References 54 Referenced 39

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Dates

Type	When
Created	17 years, 7 months ago (Jan. 2, 2008, 3:38 p.m.)
Deposited	1 year, 9 months ago (Nov. 6, 2023, 7:43 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 2, 2024, 5:09 a.m.)
Issued	51 years, 11 months ago (Sept. 1, 1973)
Published	51 years, 11 months ago (Sept. 1, 1973)
Published Online	17 years, 7 months ago (Jan. 2, 2008)
Published Print	51 years, 11 months ago (Sept. 1, 1973)

Funders 0

None

BibTeX

@article{Argyroudi_Akoyunoglou_1973, title={ON THE FORMATION OF PHOTOSYNTHETIC MEMBRANES IN BEAN PLANTS*}, volume={18}, ISSN={1751-1097}, url={http://dx.doi.org/10.1111/j.1751-1097.1973.tb06415.x}, DOI={10.1111/j.1751-1097.1973.tb06415.x}, number={3}, journal={Photochemistry and Photobiology}, publisher={Wiley}, author={Argyroudi‐Akoyunoglou, J. H. and Akoyunoglou, G.}, year={1973}, month=sep, pages={219–228} }

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  "resource": {
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      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.1973.tb06415.x"
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  "relation": {},
  "ISSN": [
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  "subject": [],
  "container-title-short": "Photochem &amp; Photobiology",
  "published": {
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}