DOI: 10.1111/j.1750-3639.2001.tb00410.x. Expression of Death‐related Proteins in Dentate Granule Cells in Human Bacterial Meningitis

Expression of Death‐related Proteins in Dentate Granule Cells in Human Bacterial Meningitis

10.1111/j.1750-3639.2001.tb00410.x

Crossref journal-article

Wiley

Brain Pathology (311)

Abstract

Neuronal apoptosis in the dentate gyrus has been observed in animal models of bacterial meningitis and in humans dying in the course of the disease. To evaluate the mechanisms of neuronal cell death, hippocampal sections of 20 patients dying from bacterial meningitis were investigated by immunohistochemistry using antibodies against the proform of caspase‐3 and the active enzyme, bcl‐2, bax and p53. In the dentate granule cell layer, the median density of neurons with an apoptotic morphology was 7.6/mm2 (0–15.6/mm2). The median density of immunoreactive neurons was 2.3/mm2 (procaspase‐3), 0.9/mm2 (activated caspase‐3), 1.8/mm2 (bcl‐2), 1.1/mm2 (bax) and 0.4/mm2 (p53). 80% of neurons immunoreactive for active caspase‐3 had an apoptotic morphology, whereas only 10% of all procaspase‐3 stained neurons showed signs of apoptosis. Apoptotic cell death is present in humans dying in the course of bacterial meningitis in the dentate gyrus of the Formatio hippocampi. Neuronal expression of caspase‐3, bcl‐2 and bax suggests an involvement of these proteins in neuronal death.

Bibliography

Gerber, J., BrÃ¼ck, W., Stadelmann, C., Bunkowski, S., Lassmann, H., & Nau, R. (2001). Expression of Deathârelated Proteins in Dentate Granule Cells in Human Bacterial Meningitis. Brain Pathology, 11(4), 422â431. Portico.

Authors 6

Joachim Gerber (first)
Wolfgang Brück (additional)
Christine Stadelmann (additional)
Stefanie Bunkowski (additional)
Hans Lassmann (additional)
Roland Nau (additional)

References 44 Referenced 23

10.1074/jbc.271.28.16850 / J Biol Chem / Fas‐induced activation of the cell death‐related protease CPP32 is inhibited by bcl‐2 and ICE family protease inhibitors by Armstrong RC (1996)
10.1016/S0006-8993(98)00286-8
10.1073/pnas.92.16.7162
10.1038/sj.cdd.4400525
10.1038/6514
10.1111/j.1365-2990.1996.tb00842.x
10.1523/JNEUROSCI.18-13-04914.1998
10.1046/j.1471-4159.1996.67010064.x
10.1074/jbc.271.9.4573
10.1523/JNEUROSCI.17-23-09172.1997
10.1002/hipo.450050103
10.1056/NEJM199301073280104
10.1016/S0022-3476(85)80342-5
10.1038/3305
10.1007/s002210050448
10.1007/s004010050753
10.1007/s004010000326
10.1089/neu.1993.10.405
10.1038/348334a0
10.1126/science.1905840
10.1111/j.1750-3639.1998.tb00134.x
10.1038/ng0897-358
{'key': 'e_1_2_1_24_2', 'first-page': '1605', 'article-title': 'Immunohistochemical analysis of in vivo patterns of expression of CPP32 (caspase‐3), a cell death protease', 'volume': '57', 'author': 'Krajewska M', 'year': '1997', 'journal-title': 'Cancer Res'} / Cancer Res / Immunohistochemical analysis of in vivo patterns of expression of CPP32 (caspase‐3), a cell death protease by Krajewska M (1997)
10.1172/JCI119084
10.1016/S0014-5793(98)00252-X
10.1086/317921
10.1016/S0361-9230(98)00024-0
10.1073/pnas.87.16.6166
10.1111/j.1750-3639.2001.tb00399.x
10.1083/jcb.139.1.205
10.1002/(SICI)1098-1063(1998)8:3<212::AID-HIPO4>3.0.CO;2-L
{'key': 'e_1_2_1_33_2', 'first-page': '3131', 'article-title': 'Identification of a p53‐dependent negative response element in the bcl‐2 gene', 'volume': '54', 'author': 'Miyashita T', 'year': '1994', 'journal-title': 'Cancer Res'} / Cancer Res / Identification of a p53‐dependent negative response element in the bcl‐2 gene by Miyashita T (1994)
10.1016/0092-8674(95)90412-3
10.1523/JNEUROSCI.18-10-03659.1998
10.1097/00005072-199903000-00006
10.1016/S0166-4328(96)00167-2
10.1016/S0968-0004(97)01085-2
10.1016/0092-8674(93)90509-O
10.1111/j.1750-3639.2001.tb00398.x
10.1542/peds.74.2.198 / Pediatr / Intellectual, neuropsychological and achievement outcomes in children six to eight years after recovery from Haemophilus influenzae meningitis by Taylor HG (1984)
10.1016/S0304-3940(00)01645-1
10.1093/infdis/162.6.1316
10.1038/352345a0
10.1097/00005072-199606000-00006

Dates

Type	When
Created	15 years, 1 month ago (July 27, 2010, 11:57 a.m.)
Deposited	1 year, 9 months ago (Nov. 19, 2023, 1:16 p.m.)
Indexed	5 months ago (March 30, 2025, 12:20 a.m.)
Issued	23 years, 11 months ago (Oct. 1, 2001)
Published	23 years, 11 months ago (Oct. 1, 2001)
Published Online	19 years, 5 months ago (April 5, 2006)
Published Print	23 years, 11 months ago (Oct. 1, 2001)

Funders 0

None

BibTeX

@article{Gerber_2001, title={Expression of Death‐related Proteins in Dentate Granule Cells in Human Bacterial Meningitis}, volume={11}, ISSN={1750-3639}, url={http://dx.doi.org/10.1111/j.1750-3639.2001.tb00410.x}, DOI={10.1111/j.1750-3639.2001.tb00410.x}, number={4}, journal={Brain Pathology}, publisher={Wiley}, author={Gerber, Joachim and Brück, Wolfgang and Stadelmann, Christine and Bunkowski, Stefanie and Lassmann, Hans and Nau, Roland}, year={2001}, month=oct, pages={422–431} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        3,
        30
      ]
    ],
    "date-time": "2025-03-30T04:20:09Z",
    "timestamp": 1743308409493
  },
  "reference-count": 44,
  "publisher": "Wiley",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            4,
            5
          ]
        ],
        "date-time": "2006-04-05T00:00:00Z",
        "timestamp": 1144195200000
      },
      "content-version": "vor",
      "delay-in-days": 1647,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        10
      ]
    ]
  },
  "abstract": "<jats:p>Neuronal apoptosis in the dentate gyrus has been observed in animal models of bacterial meningitis and in humans dying in the course of the disease. To evaluate the mechanisms of neuronal cell death, hippocampal sections of 20 patients dying from bacterial meningitis were investigated by immunohistochemistry using antibodies against the proform of caspase\u20103 and the active enzyme, bcl\u20102, bax and p53. In the dentate granule cell layer, the median density of neurons with an apoptotic morphology was 7.6/mm<jats:sup>2</jats:sup> (0\u201315.6/mm<jats:sup>2</jats:sup>). The median density of immunoreactive neurons was 2.3/mm<jats:sup>2</jats:sup> (procaspase\u20103), 0.9/mm<jats:sup>2</jats:sup> (activated caspase\u20103), 1.8/mm<jats:sup>2</jats:sup> (bcl\u20102), 1.1/mm<jats:sup>2</jats:sup> (bax) and 0.4/mm<jats:sup>2</jats:sup> (p53). 80% of neurons immunoreactive for active caspase\u20103 had an apoptotic morphology, whereas only 10% of all procaspase\u20103 stained neurons showed signs of apoptosis. Apoptotic cell death is present in humans dying in the course of bacterial meningitis in the dentate gyrus of the Formatio hippocampi. Neuronal expression of caspase\u20103, bcl\u20102 and bax suggests an involvement of these proteins in neuronal death.</jats:p>",
  "DOI": "10.1111/j.1750-3639.2001.tb00410.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2010,
        7,
        27
      ]
    ],
    "date-time": "2010-07-27T15:57:11Z",
    "timestamp": 1280246231000
  },
  "page": "422-431",
  "source": "Crossref",
  "is-referenced-by-count": 23,
  "title": "Expression of Death\u2010related Proteins in Dentate Granule Cells in Human Bacterial Meningitis",
  "prefix": "10.1111",
  "volume": "11",
  "author": [
    {
      "given": "Joachim",
      "family": "Gerber",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Wolfgang",
      "family": "Br\u00fcck",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Christine",
      "family": "Stadelmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Stefanie",
      "family": "Bunkowski",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hans",
      "family": "Lassmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Roland",
      "family": "Nau",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        4,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "850",
      "DOI": "10.1074/jbc.271.28.16850",
      "article-title": "Fas\u2010induced activation of the cell death\u2010related protease CPP32 is inhibited by bcl\u20102 and ICE family protease inhibitors",
      "volume": "271",
      "author": "Armstrong RC",
      "year": "1996",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-8993(98)00286-8"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.16.7162"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.cdd.4400525"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/6514"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2990.1996.tb00842.x"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-13-04914.1998"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1471-4159.1996.67010064.x"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.9.4573"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.17-23-09172.1997"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/hipo.450050103"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM199301073280104"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-3476(85)80342-5"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/3305"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s002210050448"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004010050753"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004010000326"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1089/neu.1993.10.405"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/348334a0"
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1905840"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1750-3639.1998.tb00134.x"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng0897-358"
    },
    {
      "key": "e_1_2_1_24_2",
      "first-page": "1605",
      "article-title": "Immunohistochemical analysis of in vivo patterns of expression of CPP32 (caspase\u20103), a cell death protease",
      "volume": "57",
      "author": "Krajewska M",
      "year": "1997",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI119084"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(98)00252-X"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/317921"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0361-9230(98)00024-0"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.16.6166"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1750-3639.2001.tb00399.x"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.139.1.205"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1098-1063(1998)8:3<212::AID-HIPO4>3.0.CO;2-L"
    },
    {
      "key": "e_1_2_1_33_2",
      "first-page": "3131",
      "article-title": "Identification of a p53\u2010dependent negative response element in the bcl\u20102 gene",
      "volume": "54",
      "author": "Miyashita T",
      "year": "1994",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90412-3"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-10-03659.1998"
    },
    {
      "key": "e_1_2_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00005072-199903000-00006"
    },
    {
      "key": "e_1_2_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0166-4328(96)00167-2"
    },
    {
      "key": "e_1_2_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(97)01085-2"
    },
    {
      "key": "e_1_2_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90509-O"
    },
    {
      "key": "e_1_2_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1750-3639.2001.tb00398.x"
    },
    {
      "key": "e_1_2_1_41_2",
      "doi-asserted-by": "crossref",
      "first-page": "198",
      "DOI": "10.1542/peds.74.2.198",
      "article-title": "Intellectual, neuropsychological and achievement outcomes in children six to eight years after recovery from Haemophilus influenzae meningitis",
      "volume": "74",
      "author": "Taylor HG",
      "year": "1984",
      "journal-title": "Pediatr"
    },
    {
      "key": "e_1_2_1_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3940(00)01645-1"
    },
    {
      "key": "e_1_2_1_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/infdis/162.6.1316"
    },
    {
      "key": "e_1_2_1_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/352345a0"
    },
    {
      "key": "e_1_2_1_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00005072-199606000-00006"
    }
  ],
  "container-title": "Brain Pathology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3639.2001.tb00410.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1750-3639.2001.tb00410.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        19
      ]
    ],
    "date-time": "2023-11-19T18:16:22Z",
    "timestamp": 1700417782000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1750-3639.2001.tb00410.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        10
      ]
    ]
  },
  "references-count": 44,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2001,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1750-3639.2001.tb00410.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1750-3639.2001.tb00410.x",
  "relation": {},
  "ISSN": [
    "1015-6305",
    "1750-3639"
  ],
  "subject": [],
  "container-title-short": "Brain Pathology",
  "published": {
    "date-parts": [
      [
        2001,
        10
      ]
    ]
  }
}