DOI: 10.1111/j.1749-6632.1988.tb25808.x. Development of an Efficient Method for Generating and Screening Active Trypsin and Trypsin Variants

Development of an Efficient Method for Generating and Screening Active Trypsin and Trypsin Variants

10.1111/j.1749-6632.1988.tb25808.x

Crossref journal-article

Wiley

Annals of the New York Academy of Sciences (311)

Bibliography

EVNIN, L. B., & CRAIK, C. S. (1988). Development of an Efficient Method for Generating and Screening Active Trypsin and Trypsin Variants. Annals of the New York Academy of Sciences, 542(1), 61â74. Portico.

Authors 2

LUKE B. EVNIN (first)
CHARLES S. CRAIK (additional)

References 40 Referenced 20

The binding site for a polypeptide chain consists of a series of subsites across the surface of the enzyme. By convention the substrate residues involved in the binding are referred to as P (peptide) and are numbered bidirectionally from the residues on either side of the scissile bond (the bond that is cleaved). The subsites abbreviated S. are numbered to match the peptide; thus the S3 subsite interacts with the P3 residue in the substrate:.
10.1146/annurev.bi.46.070177.001555
10.1146/annurev.bb.11.060182.002223
10.1107/S010876818300275X
10.1016/0022-2836(83)90078-5
10.1016/0022-2836(78)90412-6
10.1016/S0021-9258(18)89259-6 / J. Biol. Chem. / The refinement and the structure of the dimer of alpha‐chymotrypsin at 1.67 Å resolution by BLEVINS R. (1985)
10.1016/0022-2836(85)90314-6
10.1021/bi00288a012
10.1146/annurev.bb.13.060184.002321
10.1016/0022-2836(81)90465-4
10.1038/257758a0
10.1126/science.3838593
10.1021/bi00383a031
10.1093/nar/11.22.7853
{'key': 'e_1_2_1_17_2', 'volume-title': 'Molecular Cloning', 'author': 'MANIAITS T.', 'year': '1982'} / Molecular Cloning by MANIAITS T. (1982)
10.1073/pnas.78.7.4180
10.1128/jb.149.2.434-439.1982 / J. Bacteriol. / Signal sequence of alkaline phosphatase of E. coli by INOUYE H. (1982)
10.1073/pnas.82.2.488
10.1093/nar/11.6.1645
{'key': 'e_1_2_1_22_2', 'first-page': '109', 'volume-title': 'DNA Cloning: A Practical Approach', 'author': 'HANAHAN D.', 'year': '1985'} / DNA Cloning: A Practical Approach by HANAHAN D. (1985)
10.1073/pnas.74.12.5463
BIORADLABORATORIES.1987.Muta‐genein vitromutagenesis kit instruction manual.
10.1016/S0021-9258(17)44400-0 / J. Biol. Chem. / Two forms of the DNA polymerase of bacteriophage T7 by ENGLER M. (1983)
10.1111/j.1462-5822.2007.00901.x
10.1016/S0021-9258(18)89886-6 / J. Biol. Chem. / Structure of two related rat pancreatic trypsin genes by CRAIK C. (1984)
10.1016/S0021-9258(18)34133-4 / J. Biol. Chem. / Two similar but nonallelic rat pancreatic trypsinogens by MACDONALD R. (1982)
{'key': 'e_1_2_1_29_2', 'first-page': '615', 'volume-title': 'Cold Spring Harbor Symp. Quant. Biol.', 'author': 'HIGAKI J.', 'year': '1987'} / Cold Spring Harbor Symp. Quant. Biol. by HIGAKI J. (1987)
10.1016/0378-1119(82)90021-X
10.1146/annurev.bb.06.060177.001141
10.1021/ar50123a006
10.1128/jb.145.2.687-695.1981 / J. Bacteriol. / Synthesis and metabolism of uracil‐containing deoxyribonucleic acid in E. coli by WARNER H. (1981)
10.1016/S0021-9258(20)79779-6 / J. Biol. Chem. / DNA synthesis on a double‐stranded DNA template by the T4 bacteriophage DNA polymerase and T4 gene 32 DNA unwinding protein by NOSSAL N. (1974)
10.1016/S0021-9258(17)44401-2 / J. Biol. Chem. / Characterization of strand displacement catalyzed by bacteriophage T7 DNA polymerase by LECHNER R. (1983)
10.1073/pnas.82.2.503
10.1016/0092-8674(84)90283-6
10.1073/pnas.81.8.2285
{'key': 'e_1_2_1_39_2', 'first-page': '12', 'article-title': 'Use of oligonucleotides for site‐specific mutagenesis', 'volume': '2', 'author': 'CRAIK C.', 'year': '1985', 'journal-title': 'BioTechniques'} / BioTechniques / Use of oligonucleotides for site‐specific mutagenesis by CRAIK C. (1985)
10.1016/0003-2697(81)90281-5
10.1002/prot.340010406

Dates

Type	When
Created	18 years, 8 months ago (Dec. 17, 2006, 8:58 a.m.)
Deposited	1 year, 11 months ago (Sept. 25, 2023, 1:46 p.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 12:22 a.m.)
Issued	36 years, 9 months ago (Dec. 1, 1988)
Published	36 years, 9 months ago (Dec. 1, 1988)
Published Online	18 years, 8 months ago (Dec. 17, 2006)
Published Print	36 years, 9 months ago (Dec. 1, 1988)

Funders 0

None

BibTeX

@article{EVNIN_1988, title={Development of an Efficient Method for Generating and Screening Active Trypsin and Trypsin Variants}, volume={542}, ISSN={1749-6632}, url={http://dx.doi.org/10.1111/j.1749-6632.1988.tb25808.x}, DOI={10.1111/j.1749-6632.1988.tb25808.x}, number={1}, journal={Annals of the New York Academy of Sciences}, publisher={Wiley}, author={EVNIN, LUKE B. and CRAIK, CHARLES S.}, year={1988}, month=dec, pages={61–74} }

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