DOI: 10.1111/j.1748-1716.1982.tb06996.x. Volume regulation of frog skin epithelium

Volume regulation of frog skin epithelium

10.1111/j.1748-1716.1982.tb06996.x

Crossref journal-article

Wiley

Acta Physiologica Scandinavica (311)

Abstract

Previous results (MacRobbie & Ussing 1961) in combination with published values for cellular chloride concentration and for intracellular potentials show that the chloride concentration in frog skin epithelium cells is higher than predicted for equilibrium with the inside bathing solution. Both the apical and the basolateral membrane of these cells are normally almost tight to chloride, so that the maintenance of the high chloride concentration requires little work. A basolateral permeability to chloride is, however, activated by cell swelling, and the cells lose KCI. It is now shown that the KCI thus lost cannot be regained neither in the absence of sodium in the inside bath nor in the presence of furosemide. The volume regulation reactions are, however, independent of the composition of the outside bath. It is concluded that the recovery of KCI by the epithelium is due to a basolateral co‐transport of NaCl from medium to cells, combined with return of Na to the medium via the Na‐K pump. The co‐transport mechansism thus restores the high chloride concentration of the cells, but seems to be virtually dormant unless the cells have lost chloride.

Bibliography

USSING, H. H. (1982). Volume regulation of frog skin epithelium. Acta Physiologica Scandinavica, 114(3), 363â369. Portico.

Authors 1

HANS H. USSING (first)

References 24 Referenced 111

10.1152/ajplegacy.1973.224.6.1288 / Am J Physiol / Regulation of renal tubule cell volume in hypotonic media by Dellasega M. (1973)
{'key': 'e_1_2_1_3_1', 'volume-title': 'Water transport across epithelia', 'author': 'Dorge A.', 'year': '1981'} / Water transport across epithelia by Dorge A. (1981)
10.1073/pnas.51.4.569
10.1111/j.1749-6632.1980.tb47160.x
{'key': 'e_1_2_1_6_1', 'first-page': '301', 'volume-title': 'Cell membrane receptors for drugs and hormones', 'author': 'Geck P.', 'year': '1978'} / Cell membrane receptors for drugs and hormones by Geck P. (1978)
10.1085/jgp.74.1.105
10.1002/jcp.1040840113
{'key': 'e_1_2_1_9_1', 'first-page': '285', 'volume-title': 'Transport of ions and water in animals', 'author': 'Hoffmann E. K.', 'year': '1977'} / Transport of ions and water in animals by Hoffmann E. K. (1977)
{'key': 'e_1_2_1_10_1', 'first-page': '94', 'article-title': 'Anioncation co‐transport and volume regulation in Ehrlich ascites tumor cells', 'volume': '319', 'author': 'Hoffmann E. K.', 'year': '1981', 'journal-title': 'J Physiol'} / J Physiol / Anioncation co‐transport and volume regulation in Ehrlich ascites tumor cells by Hoffmann E. K. (1981)
10.1111/j.1748-1716.1958.tb01563.x
10.1085/jgp.58.4.372
10.1146/annurev.ph.43.030181.002425
10.1007/BF01870645
10.1111/j.1748-1716.1961.tb02293.x
10.1007/BF01067010
10.1007/BF02007639
10.1152/ajplegacy.1973.225.2.467 / Am J Physiol / Coupled sodium‐chloride influxes across the brush border of rabbit ileum by Nellans H. N. (1973)
10.1111/j.1748-1716.1979.tb06462.x
10.1007/BF01869897
10.1016/0014-4827(73)90448-5
10.1111/j.1748-1716.1965.tb04052.x
10.1007/BF02025995
{'key': 'e_1_2_1_24_1', 'volume-title': 'Osmotic and volume regulation, Alfred Benzon Symposium XI', 'author': 'Ussing H. H.', 'year': '1978'} / Osmotic and volume regulation, Alfred Benzon Symposium XI by Ussing H. H. (1978)
{'key': 'e_1_2_1_25_1', 'first-page': '484', 'article-title': 'Nature of shunt path and active sodium transport path through frog skin epithelium', 'volume': '61', 'author': 'Ussing H.H.', 'year': '1964', 'journal-title': 'Acta Physiol Scand'} / Acta Physiol Scand / Nature of shunt path and active sodium transport path through frog skin epithelium by Ussing H.H. (1964)

Dates

Type	When
Created	16 years, 8 months ago (Dec. 9, 2008, 8:32 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 10:07 a.m.)
Indexed	1 week, 6 days ago (Aug. 23, 2025, 1:04 a.m.)
Issued	43 years, 6 months ago (March 1, 1982)
Published	43 years, 6 months ago (March 1, 1982)
Published Online	16 years, 8 months ago (Dec. 8, 2008)
Published Print	43 years, 6 months ago (March 1, 1982)

Funders 0

None

BibTeX

@article{USSING_1982, title={Volume regulation of frog skin epithelium}, volume={114}, ISSN={1365-201X}, url={http://dx.doi.org/10.1111/j.1748-1716.1982.tb06996.x}, DOI={10.1111/j.1748-1716.1982.tb06996.x}, number={3}, journal={Acta Physiologica Scandinavica}, publisher={Wiley}, author={USSING, HANS H.}, year={1982}, month=mar, pages={363–369} }

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