DOI: 10.1111/j.1748-1716.1980.tb06543.x. Muscarinic autoreceptor regulates acetylcholine release in rat hippocampus: in vitro evidence

Muscarinic autoreceptor regulates acetylcholine release in rat hippocampus: in vitro evidence

10.1111/j.1748-1716.1980.tb06543.x

Crossref journal-article

Wiley

Acta Physiologica Scandinavica (311)

Abstract

Release of 3H‐ACh from isolated nerve endings of rat hippocampus was evoked by incubation in Krebs‐Ringer's buffer containing 25 or 35 mM potassium. The release was Ca2+‐dependent and could be inhibited by Mg2+ (20 mM). The muscarinic antagonist, atropine (10‐10–10‐6 M), enhanced 3H‐ACh‐release. The muscarinic agonist, carbachol (10‐5–10‐3 M) inhibited 3H‐ACh release via interaction with muscarinic receptors: this effect could be blocked by atropine (10‐6 M). The presence of the feed‐back regulation of 3H‐ACh release in a cell‐free preparation provides further evidence that the presynaptic regulation is exerted by muscarinic autoreceptors localized on the cholinergic nerve ending itself. The feed back inhibition of the 3H‐ACh release does not require the presence of intact neurons or neuronal loops as tetrodotoxin (2.5. 10‐6 M) does not affect the above results.

Authors 2

Ö. NORDSTRÖM (first)
T. BARTFAI (additional)

References 29 Referenced 131

10.1016/S0021-9258(17)33682-7 / J Biol Chem / Muscarinic acetylcholine receptor from rat brain. Partial purification and characterization by ALBERTS P. (1976)
10.1139/o61-081
10.1111/j.1476-5381.1974.tb09718.x
10.1113/jphysiol.1969.sp008890
10.1002/neu.480020305
10.1016/0005-2736(74)90268-5
10.1139/y75-146
10.1007/BF00632637 / NaunynSchmiedeberg's Arch Pharmacol / Inhibition of oxotremorine of acetylcholine resting release from guinea‐pig longitudinal muscle strips by KILBINGER H. (1975)
{'key': 'e_1_2_1_9_1', 'first-page': '7', 'volume-title': 'Handbook of chemical assay methods', 'author': 'LADINSKY H.', 'year': '1974'} / Handbook of chemical assay methods by LADINSKY H. (1974)
10.1016/S0021-9258(19)52451-6
10.1073/pnas.76.12.6336
10.1113/jphysiol.1963.sp007045
10.1111/j.1476-5381.1970.tb10622.x
10.1016/0006-8993(75)90048-7
10.1016/0006-8993(79)90057-X
{'key': 'e_1_2_1_16_1', 'first-page': '660', 'article-title': 'The stimulating action of atropine on the release of acetylcholine by rat cerebral cortex in vitro', 'volume': '14', 'author': 'POLAK R. L.', 'year': '1971', 'journal-title': 'Brit J Pharmacol'} / Brit J Pharmacol / The stimulating action of atropine on the release of acetylcholine by rat cerebral cortex in vitro by POLAK R. L. (1971)
10.1016/0006-2952(66)90176-6
10.1139/y77-121
{'key': 'e_1_2_1_19_1', 'first-page': '440', 'article-title': "Influence of atropine and morphine on the liberation of acetylcholine from the guinea pig's intestine", 'volume': '254', 'author': 'SCHAUMANN W.', 'year': '1956', 'journal-title': 'Nature (Lond.)'} / Nature (Lond.) / Influence of atropine and morphine on the liberation of acetylcholine from the guinea pig's intestine by SCHAUMANN W. (1956)
10.1073/pnas.73.8.2664
10.1016/0006-8993(77)90929-5
10.1111/j.1471-4159.1976.tb06472.x
10.1016/0006-2952(77)90174-5
{'key': 'e_1_2_1_24_1', 'first-page': '179', 'article-title': 'Basic mechanisms and local feedback. Control of secretion of adrenergic and cholinergic neurotransmitters', 'volume': '6', 'author': 'STJÄRNE L.', 'year': '1975', 'journal-title': 'In: Handbook of psychopharmacology'} / In: Handbook of psychopharmacology / Basic mechanisms and local feedback. Control of secretion of adrenergic and cholinergic neurotransmitters by STJÄRNE L. (1975)
10.1139/y64-036
{'key': 'e_1_2_1_26_1', 'first-page': '49', 'volume-title': 'Cholinergic mechanisms and psychopharmacology', 'author': 'SZERB J. C.', 'year': '1977'} / Cholinergic mechanisms and psychopharmacology by SZERB J. C. (1977)
10.1038/newbio241121a0
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10.1073/pnas.71.5.1725

Dates

Type	When
Created	16 years, 8 months ago (Dec. 9, 2008, 7:53 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 8:44 a.m.)
Indexed	8 hours, 33 minutes ago (Sept. 4, 2025, 10:03 a.m.)
Issued	45 years, 5 months ago (April 1, 1980)
Published	45 years, 5 months ago (April 1, 1980)
Published Online	16 years, 8 months ago (Dec. 8, 2008)
Published Print	45 years, 5 months ago (April 1, 1980)

Funders 0

None

BibTeX

@article{NORDSTR_M_1980, title={Muscarinic autoreceptor regulates acetylcholine release in rat hippocampus: in vitro evidence}, volume={108}, ISSN={1365-201X}, url={http://dx.doi.org/10.1111/j.1748-1716.1980.tb06543.x}, DOI={10.1111/j.1748-1716.1980.tb06543.x}, number={4}, journal={Acta Physiologica Scandinavica}, publisher={Wiley}, author={NORDSTRÖM, Ö. and BARTFAI, T.}, year={1980}, month=apr, pages={347–353} }

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