DOI: 10.1111/j.1748-1716.1977.tb10381.x. Effects of Acetylsalicylate on Alkalinization, Acid Secretion and Electrogenic Properties in the Isolated Gastric Mucosa

Effects of Acetylsalicylate on Alkalinization, Acid Secretion and Electrogenic Properties in the Isolated Gastric Mucosa

10.1111/j.1748-1716.1977.tb10381.x

Crossref journal-article

Wiley

Acta Physiologica Scandinavica (311)

Abstract

AbstractThe effects of acetylsalicylate (ASA) on the in vitro secretory and electrical properties of Necturus and Rana temporaria gastric mucosa have been studied. The gastric antrum alkalinized the luminal surface, while in the fundus it is likely that acidification and alkalinization occur simultaneously and that net secretion is due to the dominance of one or other of these processes. The histamine H2 receptor antagonist Metiamide was used to inhibit acid secretion for studies on fundic alkalinization in Rana temporaria. Submucosal application of 3 mM ASA for 30 min markedly reduced alkalinization in the antrum and the frog fundus. Following removal of ASA there was only partial recovery of this secretion. The drug caused slight inhibition of spontaneous acid secretion in Necturus fundus but not of histamine‐stimulated acid secretion in the frog fundus. Following salicylate removal, the rate of acid secretion increased to a higher level than before administration in both tissues. There was a small flux of ASA across the mucosa which was greatest in the acid secreting frog fundus (4.38μmol cm‐2 min‐1) and least in the antrum (2.19μmol cm‐2 min‐1). Exposure of the gastric mucosa to ASA was generally associated with a fall in transmucosal electric potential difference and short‐circuit current together with an increase in electrical resistance. It is proposed that the greater sensitivity of alkalinization to ASA is responsible for the apparent increase in the rate of acid secretion which occurred.

Bibliography

Garner, A. (1977). Effects of Acetylsalicylate on Alkalinization, Acid Secretion and Electrogenic Properties in the Isolated Gastric Mucosa. Acta Physiologica Scandinavica, 99(3), 281â291. Portico.

Authors 1

Andrew Garner (first)

References 30 Referenced 39

10.1152/ajplegacy.1975.229.5.1414 / Amer. J. Physiol. / Interstitial fluid pressure in canine gastric mucosa by Altamirano M. (1975)
{'key': 'e_1_2_1_3_1', 'first-page': '379', 'article-title': 'A study of the gastric lesions induced by aspirin in laboratory animals', 'volume': '152', 'author': 'Anderson K. W.', 'year': '1964', 'journal-title': 'Arch. int. Pharmacodyn.'} / Arch. int. Pharmacodyn. / A study of the gastric lesions induced by aspirin in laboratory animals by Anderson K. W. (1964)
10.1111/j.1748-1716.1976.tb10281.x
10.1016/S0016-5085(19)33761-8 / Gastroenterology / Effects of chelation of calcium on the gastric mucosal barrier by Chung R. S. K. (1966)
10.1111/j.2042-7158.1966.tb07888.x
10.1016/S0016-5085(64)80125-6
10.1016/S0016-5085(66)80027-6
10.1056/NEJM196706082762308
10.1016/S0016-5085(69)80150-2 / Gastroenterology / Gastric mucosal haemorrhage in dogs, effects of acid, aspirin and alcohol by Davenport H. W. (1969)
10.1016/0005-2736(71)90281-1
{'key': 'e_1_2_1_12_1', 'first-page': '102', 'volume-title': 'Gastric Hydrogen Ion Secretion', 'author': 'Flemström G.', 'year': '1976'} / Gastric Hydrogen Ion Secretion by Flemström G. (1976)
Flemström G. Stimulation of gastric alkaline secretion by carbacholine and db‐cGMP.Symposium on the Biochemistry of Membrane Transport Zurich Switzerland.FEBS Letters.1976. p.49.
10.1016/S0016-5085(73)80039-3 / Gastroenterology / Dextran permeability, electrical properties and H+‐secretion in isolated frog gastric mucosa after acetylsalicylic acid by Flemström G. (1973)
10.1152/ajplegacy.1975.228.4.1188 / Amer. J. Physiol. / Ion transport by amphibian antrum in vitro by Flemstrom G. (1975)
10.1111/j.1748-1716.1971.tb05073.x
10.1016/S0016-5085(61)80070-X / Gastroenterology / Fecal blood loss produced by oral and intravenous administration of various salicylates by Grossman M. I. (1961)
10.1016/S0016-5085(19)33594-2 / Gastroenterology / Effect of aspirin and related compounds on the fine structure of mouse gastric mucosa by Hinoson D. J. (1971)
{'key': 'e_1_2_1_19_1', 'first-page': '1353', 'article-title': 'Introduction: the natural history of the isolated bullfrog mucosa', 'volume': '24', 'author': 'Hogben C. A. M.', 'year': '1965', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Introduction: the natural history of the isolated bullfrog mucosa by Hogben C. A. M. (1965)
10.3181/00379727-125-32066
10.1152/ajplegacy.1973.225.3.521 / Amer. J. Physiol. / Effects of salicylate and related compounds on gastric HC1 secretion by Kasbekar D. K. (1973)
10.1136/gut.5.3.230
10.1038/253053a0
{'key': 'e_1_2_1_24_1', 'first-page': '804', 'article-title': 'Microelectrode studies of aspirin damage to gastric mucosa', 'volume': '62', 'author': 'Sachs G.', 'year': '1972', 'journal-title': 'Gastroenterology'} / Gastroenterology / Microelectrode studies of aspirin damage to gastric mucosa by Sachs G. (1972)
10.1056/NEJM197109232851304
10.1007/BF01869973
10.1085/jgp.65.5.645
10.1001/archinte.1968.03640060013003 / Arch. intern. Med. / Aspirin injury to the gastric mucosa by Thorsen W. B. (1968)
10.1007/BF00342653
10.1152/ajplegacy.1965.209.1.127 / Amer. J. Physiol. / Effect of acetazolamide on gastric mucosa in canine vivo‐vitro preparations by Werther J. L. (1965)
10.1007/BF01870189

Dates

Type	When
Created	16 years, 8 months ago (Dec. 8, 2008, 3:31 p.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 1:44 p.m.)
Indexed	1 year, 9 months ago (Nov. 11, 2023, 7:08 p.m.)
Issued	48 years, 6 months ago (March 1, 1977)
Published	48 years, 6 months ago (March 1, 1977)
Published Online	16 years, 8 months ago (Dec. 8, 2008)
Published Print	48 years, 6 months ago (March 1, 1977)

Funders 0

None

BibTeX

@article{Garner_1977, title={Effects of Acetylsalicylate on Alkalinization, Acid Secretion and Electrogenic Properties in the Isolated Gastric Mucosa}, volume={99}, ISSN={1365-201X}, url={http://dx.doi.org/10.1111/j.1748-1716.1977.tb10381.x}, DOI={10.1111/j.1748-1716.1977.tb10381.x}, number={3}, journal={Acta Physiologica Scandinavica}, publisher={Wiley}, author={Garner, Andrew}, year={1977}, month=mar, pages={281–291} }

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