DOI: 10.1111/j.1748-1716.1971.tb04915.x. Effects of Variations in the Extracellular Osmolality on the Ionic Permeability of Vascular Smooth Muscle

Effects of Variations in the Extracellular Osmolality on the Ionic Permeability of Vascular Smooth Muscle

10.1111/j.1748-1716.1971.tb04915.x

Crossref journal-article

Wiley

Acta Physiologica Scandinavica (311)

Abstract

AbstractThe effect of osmolality variations on the membrane permeability of vascular smooth muscle to sodium and potassium was investigated by studying the washout of 24Na and 41K at different extracellular tonicities. The K efflux in normal Krebs was 4.1 10‐12 M/cm2 sec at 37° C and 2.0 10‐12 M/cm2 sec at 15° C. The corresponding Pk values were 9.4 10‐8 and 5.3 10‐8 cm/sec, respectively, assuming a membrane potential of 50 mV at 37° C and 40 mV at 15° C.A substantial fraction of the sodium content of the portal vein prevails in an osmotically inactive bound form, as demonstrated in uptake studies on preparations where cell membranes had been destroyed so as to give a homogenous tissue fluid phase (Haljamäe et al. 1970). The present 24Na washout experiments performed on “normal” portal vein tissues were for this reason completed with studies of 24Na efflux from such damaged veins. Evidence is presented for the existence of one intra‐cellular and one extracellular fraction of bound sodium. Subtraction of that component in the washout curve which represented the intracellularly bound Na fraction would yield the exchange kinetics of the “free” intracellular sodium. The magnitude and efflux of [Na+]1, thus calculated, amounted to 13.1 meq/l and 1.2 10‐12 M/cm2 sec at 15° C.Assuming, on the basis of indirect experimental evidence, that Na influx equals Na efflux in the experimental situation, a passive permeability coefficient for Na of 4.3 10‐9 cm/sec was calculated, giving a Pna/Pk ratio of 0.081. The washout experiments performed in anisosmotic media indicated that this ratio increased when osmolality was lowered, which might in part explain the strong enhancement of the spontaneous activity induced by hypotonicity. The results did not, however, indicate any clearcut alteration in either Pna, or Pk when the muscle cells were exposed to hypertonicity.

Bibliography

Jonsson, O. (1971). Effects of Variations in the Extracellular Osmolality on the Ionic Permeability of Vascular Smooth Muscle. Acta Physiologica Scandinavica, 81(3), 405â421. Portico.

Authors 1

Olof Jonsson (first)

References 38 Referenced 9

10.1111/j.1748-1716.1969.tb04402.x
10.1113/jphysiol.1956.sp005494
{'key': 'e_1_2_1_4_1', 'first-page': '166', 'volume-title': 'Smooth muscle', 'author': 'Brading A. F.', 'year': '1970'} / Smooth muscle by Brading A. F. (1970)
10.1113/jphysiol.1969.sp008712
10.1113/jphysiol.1969.sp008700
{'key': 'e_1_2_1_7_1', 'first-page': '30', 'article-title': 'The action potential of the guinea‐pig taenia coli in low sodium solution', 'volume': '197', 'author': 'Brading A. F.', 'year': '1968', 'journal-title': 'J. Physiol. (Lond.)'} / J. Physiol. (Lond.) / The action potential of the guinea‐pig taenia coli in low sodium solution by Brading A. F. (1968)
10.1113/jphysiol.1966.sp007883
10.1111/j.1476-5381.1966.tb01897.x
10.1113/jphysiol.1963.sp007250
10.1113/jphysiol.1969.sp008960
10.1113/jphysiol.1961.sp006706
10.1113/jphysiol.1962.sp006985
10.3181/00379727-113-28509
10.1152/ajplegacy.1965.209.5.955 / Amer. J. Physiol / Sodium exchange and distribution in the arterial wall by Garrahan P. (1965)
10.1085/jgp.27.1.37
10.1113/jphysiol.1962.sp006986
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10.1113/jphysiol.1961.sp006778
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10.1111/j.1748-1716.1970.tb04661.x
10.1152/ajplegacy.1960.199.5.783 / Amer. J. Physiol / Bound sodium in artery wall by Headings V. E. (1960)
10.1111/j.1365-201X.1968.tb10855.x
10.1111/j.1748-1716.1969.tb04563.x
10.1111/j.1748-1716.1969.tb04564.x
{'key': 'e_1_2_1_27_1', 'article-title': 'Effects of variations in the extracellular osmolality on the permeability to nonelectrolytes of vascular smooth muscle', 'author': 'Jonsson O.', 'year': '1971', 'journal-title': 'Acta physiol. scand'} / Acta physiol. scand / Effects of variations in the extracellular osmolality on the permeability to nonelectrolytes of vascular smooth muscle by Jonsson O. (1971)
10.1113/jphysiol.1968.sp008401
10.1113/jphysiol.1951.sp004557
{'key': 'e_1_2_1_30_1', 'first-page': '310', 'article-title': 'The effects of hyperosmolarity on intact and isolated vascular smooth muscle. Possible role in exercise hypere‐mia', 'volume': '4', 'author': 'Mellander S.', 'year': '1967', 'journal-title': 'Angiologica'} / Angiologica / The effects of hyperosmolarity on intact and isolated vascular smooth muscle. Possible role in exercise hypere‐mia by Mellander S. (1967)
10.1152/ajplegacy.1967.213.1.25 / Amer. J. Physiol / Electrical activity of single vascular smooth muscle fibres by Nakajima A. (1967)
10.1126/science.152.3718.97
{'key': 'e_1_2_1_33_1', 'first-page': '1', 'article-title': 'Quoted by E. Schoffeniels, in Ionic composition of the arterial wall', 'volume': '6', 'author': 'Rorive G.', 'year': '1969', 'journal-title': 'Angiologica'} / Angiologica / Quoted by E. Schoffeniels, in Ionic composition of the arterial wall by Rorive G. (1969)
{'key': 'e_1_2_1_34_1', 'first-page': '1', 'article-title': 'Ionic composition of the arterial wall', 'volume': '6', 'author': 'Schoffeniels E.', 'year': '1969', 'journal-title': 'Angiologica'} / Angiologica / Ionic composition of the arterial wall by Schoffeniels E. (1969)
{'key': 'e_1_2_1_35_1', 'first-page': '39', 'article-title': 'Factors influencing the 42K efflux from the smooth muscie of guinea‐pig taenia coli', 'volume': '188', 'author': 'Setekleiv J.', 'year': '1967', 'journal-title': 'J. Physiol. (Lond.)'} / J. Physiol. (Lond.) / Factors influencing the 42K efflux from the smooth muscie of guinea‐pig taenia coli by Setekleiv J. (1967)
10.1152/ajplegacy.1968.215.3.723 / Amer. J. Physiol / Membrane ion conductances of frog sartorius fibres as a function of tonicity by Sperelakis N. (1968)
10.1016/0024-3205(69)90268-9
10.1113/jphysiol.1966.sp007876
10.1111/j.1748-1716.1965.tb04052.x

Dates

Type	When
Created	16 years, 8 months ago (Dec. 8, 2008, 9:20 p.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 9:10 a.m.)
Indexed	11 months, 4 weeks ago (Sept. 8, 2024, 2:21 a.m.)
Issued	54 years, 6 months ago (March 1, 1971)
Published	54 years, 6 months ago (March 1, 1971)
Published Online	16 years, 8 months ago (Dec. 8, 2008)
Published Print	54 years, 6 months ago (March 1, 1971)

Funders 0

None

BibTeX

@article{Jonsson_1971, title={Effects of Variations in the Extracellular Osmolality on the Ionic Permeability of Vascular Smooth Muscle}, volume={81}, ISSN={1365-201X}, url={http://dx.doi.org/10.1111/j.1748-1716.1971.tb04915.x}, DOI={10.1111/j.1748-1716.1971.tb04915.x}, number={3}, journal={Acta Physiologica Scandinavica}, publisher={Wiley}, author={Jonsson, Olof}, year={1971}, month=mar, pages={405–421} }

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