DOI: 10.1111/j.1742-4658.2006.05168.x. Regional and segmental flexibility of antibodies in interaction with antigens of different size

Regional and segmental flexibility of antibodies in interaction with antigens of different size

10.1111/j.1742-4658.2006.05168.x

Crossref journal-article

Wiley

The FEBS Journal (311)

Abstract

The interaction of antibodies (Abs) with protein antigens (Ags) of different size, such as hen egg white lysozyme, ovalbumin, and bovine serum albumin, was examined using analytical ultracentrifugation, electrospray ionization time‐of‐flight mass spectrometry, and surface plasmon resonance in order to estimate regional and segmental Ab flexibility. When both Abs and Ags were free in solution, sedimentation equilibrium and surface plasmon resonance analyses showed the formation of an Ag2Ab1 complexes regardless of Ag size, suggesting that the Fab arms were able to move to avoid interference between Ags bound to Ab combining sites. The Ag2Ab1 complex, as well as the Ag1Ab1 complex, was observed by MS. However, when Abs were immobilized on the surface of a sensor chip through the Fc region, the stoichiometry of the Ag–Ab complex was dependent on the Ag size; Ag2Ab1 forming with hen egg white lysozyme and Ag1Ab1 with ovalbumin and bovine serum albumin. These results indicated that immobilization of the Fc region reduces the dynamic range of the Fab arms and results in interference from the first Ag bound to either combining site, which in turn prevents the binding of the second Ag to the other combining site. Our results allow us to propose that the Fab arms of B‐cell receptors whose Fc regions are immobilized on cell surface have a reduced dynamic range.

Bibliography

Oda, M., Uchiyama, S., Robinson, C. V., Fukui, K., Kobayashi, Y., & Azuma, T. (2006). Regional and segmental flexibility of antibodies in interaction with antigens of different size. The FEBS Journal, 273(7), 1476â1487. Portico.

Authors 6

Masayuki Oda (first)
Susumu Uchiyama (additional)
Carol V. Robinson (additional)
Kiichi Fukui (additional)
Yuji Kobayashi (additional)
Takachika Azuma (additional)

References 24 Referenced 43

Dates

Type	When
Created	19 years, 5 months ago (March 13, 2006, 5:35 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 3:22 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 2, 2025, 1:25 a.m.)
Issued	19 years, 5 months ago (March 13, 2006)
Published	19 years, 5 months ago (March 13, 2006)
Published Online	19 years, 5 months ago (March 13, 2006)
Published Print	19 years, 4 months ago (April 1, 2006)

Funders 0

None

BibTeX

@article{Oda_2006, title={Regional and segmental flexibility of antibodies in interaction with antigens of different size}, volume={273}, ISSN={1742-4658}, url={http://dx.doi.org/10.1111/j.1742-4658.2006.05168.x}, DOI={10.1111/j.1742-4658.2006.05168.x}, number={7}, journal={The FEBS Journal}, publisher={Wiley}, author={Oda, Masayuki and Uchiyama, Susumu and Robinson, Carol V. and Fukui, Kiichi and Kobayashi, Yuji and Azuma, Takachika}, year={2006}, month=mar, pages={1476–1487} }

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