DOI: 10.1111/j.1742-4658.2005.04951.x. The chloroplast ClpP complex in <i>Chlamydomonas reinhardtii</i> contains an unusual high molecular mass subunit with a large apical domain

The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain

10.1111/j.1742-4658.2005.04951.x

Crossref journal-article

Wiley

The FEBS Journal (311)

Abstract

The composition of the chloroplast‐localized protease complex, ClpP, from the green alga Chlamydomonas reinhardtii was characterized by nondenaturing electrophoresis, immunoblotting and MS. The detected ClpP complex has a native mass of ≈ 540 kDa, which is ≈ 200 kDa higher than ClpP complexes in higher plant chloroplasts, mitochondria or bacteria. The 540‐kDa ClpP complex contains two nuclear‐encoded ClpP proteins (ClpP3 and P5) and five ClpR (R1, R2, R3, R4 and R6) proteins, as well two proteins, ClpP1L and ClpP1H, both probably derived from the plastid clpP1 gene. ClpP1H is 59 kDa and contains a ≈ 30‐kDa insertion sequence (IS1) not found in other ClpP proteins, responsible for the high MW of the complex. Based on comparison with other sequences, IS1 protrudes as an additional domain on the apical surface of the ClpP/R complex, probably preventing interaction with the HSP100 chaperone. ClpP1L is a 25‐kDa protein similar in size to other ClpP proteins and could arise by post‐translational processing of ClpP1H. Chloramphenicol‐chase experiments show that ClpP1L and ClpP1H have a similar half‐life, indicating that both are stable components of the complex. The structure of the ClpP complex is further discussed in conjunction with a phylogenetic analysis of the ClpP/R genes. A model is proposed for the evolution of the algal and plant complex from its cyanobacterial ancestor.

Bibliography

Majeran, W., Friso, G., van Wijk, K. J., & Vallon, O. (2005). The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain. The FEBS Journal, 272(21), 5558â5571. Portico.

Authors 4

Wojciech Majeran (first)
Giulia Friso (additional)
Klaas Jan van Wijk (additional)
Olivier Vallon (additional)

References 51 Referenced 31

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Dates

Type	When
Created	19 years, 10 months ago (Oct. 13, 2005, 9:19 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 5:48 p.m.)
Indexed	1 year, 1 month ago (June 30, 2024, 2:30 p.m.)
Issued	19 years, 10 months ago (Oct. 13, 2005)
Published	19 years, 10 months ago (Oct. 13, 2005)
Published Online	19 years, 10 months ago (Oct. 13, 2005)
Published Print	19 years, 9 months ago (Nov. 1, 2005)

Funders 0

None

BibTeX

@article{Majeran_2005, title={The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain}, volume={272}, ISSN={1742-4658}, url={http://dx.doi.org/10.1111/j.1742-4658.2005.04951.x}, DOI={10.1111/j.1742-4658.2005.04951.x}, number={21}, journal={The FEBS Journal}, publisher={Wiley}, author={Majeran, Wojciech and Friso, Giulia and van Wijk, Klaas Jan and Vallon, Olivier}, year={2005}, month=oct, pages={5558–5571} }

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