DOI: 10.1111/j.1600-0854.2008.00841.x. Arginine/Lysine Residues in the Cytoplasmic Tail Promote ER Export of Plant Glycosylation Enzymes

Arginine/Lysine Residues in the Cytoplasmic Tail Promote ER Export of Plant Glycosylation Enzymes

10.1111/j.1600-0854.2008.00841.x

Crossref journal-article

Wiley

Traffic (311)

Abstract

Plant N‐glycan processing enzymes are arranged along the early secretory pathway, forming an assembly line to facilitate the step‐by‐step modification of oligosaccharides on glycoproteins. Thus, these enzymes provide excellent tools to study signals and mechanisms, promoting their localization and retention in the endoplasmic reticulum (ER) and Golgi apparatus. Herein, we focused on a detailed investigation of amino acid sequence motifs present in their short cytoplasmic tails in respect to ER export. Using site‐directed mutagenesis, we determined that single arginine/lysine residues within the cytoplasmic tail are sufficient to promote rapid Golgi targeting of Golgi‐resident N‐acetylglucosaminyltransferase I (GnTI) and α‐mannosidase II (GMII). Furthermore, we reveal that an intact ER export motif is essential for proper in vivo function of GnTI. Coexpression studies with Sar1p provided evidence for COPII‐dependent transport of GnTI to the Golgi. Our data provide evidence that efficient ER export of Golgi‐resident plant N‐glycan processing enzymes occurs through a selective mechanism based on recognition of single basic amino acids present in their cytoplasmic tails.

Bibliography

Schoberer, J., Vavra, U., Stadlmann, J., Hawes, C., Mach, L., Steinkellner, H., & Strasser, R. (2008). Arginine/Lysine Residues in the Cytoplasmic Tail Promote ER Export of Plant Glycosylation Enzymes. Traffic, 10(1), 101â115. Portico.

Authors 7

Jennifer Schoberer (first)
Ulrike Vavra (additional)
Johannes Stadlmann (additional)
Chris Hawes (additional)
Lukas Mach (additional)
Herta Steinkellner (additional)
Richard Strasser (additional)

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Dates

Type	When
Created	16 years, 10 months ago (Oct. 7, 2008, 12:13 p.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 12:09 p.m.)
Indexed	4 weeks ago (July 26, 2025, 5:17 a.m.)
Issued	16 years, 7 months ago (Dec. 30, 2008)
Published	16 years, 7 months ago (Dec. 30, 2008)
Published Online	16 years, 7 months ago (Dec. 30, 2008)
Published Print	16 years, 7 months ago (Jan. 1, 2009)

Funders 0

None

BibTeX

@article{Schoberer_2008, title={Arginine/Lysine Residues in the Cytoplasmic Tail Promote ER Export of Plant Glycosylation Enzymes}, volume={10}, ISSN={1600-0854}, url={http://dx.doi.org/10.1111/j.1600-0854.2008.00841.x}, DOI={10.1111/j.1600-0854.2008.00841.x}, number={1}, journal={Traffic}, publisher={Wiley}, author={Schoberer, Jennifer and Vavra, Ulrike and Stadlmann, Johannes and Hawes, Chris and Mach, Lukas and Steinkellner, Herta and Strasser, Richard}, year={2008}, month=dec, pages={101–115} }

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