DOI: 10.1111/j.1600-0854.2008.00789.x. The Luminal N‐Terminus of Yeast Nvj1 is an Inner Nuclear Membrane Anchor

The Luminal N‐Terminus of Yeast Nvj1 is an Inner Nuclear Membrane Anchor

10.1111/j.1600-0854.2008.00789.x

Crossref journal-article

Wiley

Traffic (311)

Abstract

The endoplasmic reticulum (ER) in Saccharomyces cerevisiae is largely divided between perinuclear and cortical compartments. Yeast Nvj1 localizes exclusively to small patches on the perinuclear ER where it interacts with Vac8 in the vacuole membrane to form nucleus–vacuole (NV) junctions. Three regions of Nvj1 mediate the biogenesis of NV junctions. A membrane‐spanning domain targets the protein to the ER. The C‐terminus binds Vac8 in the vacuole membrane, which induces the clustering of both proteins into NV junctions. The luminal N‐terminus is required for strict perinuclear localization. Three‐dimensional cryo‐electron tomography reveals that Nvj1 clamps the separation between the two nuclear membranes to half the width of bulk nuclear envelope. The N‐terminus contains a hydrophobic sequence bracketed by basic residues that resembles outer mitochondrial membrane signal‐anchors. The hydrophobic sequence can be scrambled or reversed without affecting function. Mutations that reduce the hydrophobicity of the core sequence or affect the distribution of basic residues cause mislocalization to the cortical ER. We conclude that the N‐terminus of Nvj1 is a retention sequence that bridges the perinuclear lumen and inserts into the inner nuclear membrane.

Bibliography

Millen, J. I., Pierson, J., Kvam, E., Olsen, L. J., & Goldfarb, D. S. (2008). The Luminal NâTerminus of Yeast Nvj1 is an Inner Nuclear Membrane Anchor. Traffic, 9(10), 1653â1664. Portico.

Authors 5

Jonathan I. Millen (first)
Jason Pierson (additional)
Erik Kvam (additional)
Lars J. Olsen (additional)
David S. Goldfarb (additional)

References 45 Referenced 29

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{'key': 'e_1_2_6_45_2', 'first-page': '17', 'volume-title': 'Electron Tomography: Methods for three‐dimensional visualization of structures in the cell', 'author': 'Luther PK', 'year': '2005'} / Electron Tomography: Methods for three‐dimensional visualization of structures in the cell by Luther PK (2005)
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Dates

Type	When
Created	17 years ago (Aug. 6, 2008, 2:57 a.m.)
Deposited	1 year, 10 months ago (Oct. 11, 2023, 7:04 a.m.)
Indexed	11 months, 4 weeks ago (Aug. 23, 2024, 4:35 a.m.)
Issued	16 years, 11 months ago (Sept. 15, 2008)
Published	16 years, 11 months ago (Sept. 15, 2008)
Published Online	16 years, 11 months ago (Sept. 15, 2008)
Published Print	16 years, 10 months ago (Oct. 1, 2008)

Funders 0

None

BibTeX

@article{Millen_2008, title={The Luminal N‐Terminus of Yeast Nvj1 is an Inner Nuclear Membrane Anchor}, volume={9}, ISSN={1600-0854}, url={http://dx.doi.org/10.1111/j.1600-0854.2008.00789.x}, DOI={10.1111/j.1600-0854.2008.00789.x}, number={10}, journal={Traffic}, publisher={Wiley}, author={Millen, Jonathan I. and Pierson, Jason and Kvam, Erik and Olsen, Lars J. and Goldfarb, David S.}, year={2008}, month=sep, pages={1653–1664} }

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