DOI: 10.1111/j.1600-0609.1990.tb00340.x. Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid

10.1111/j.1600-0609.1990.tb00340.x

Crossref journal-article

Wiley

European Journal of Haematology (311)

Abstract

Abstract: A new malignant human T‐cell line‐labelled PFI‐285‐has been isolated from a boy with malignant lymphoma. Morphologically, the cells had characteristics of malignant lymphoid cells. The cells presented surface antigens as early cortical lymphocytes and proliferated non‐adherently as single cells, independent of T‐cell growth factor (IL‐2), in liquid culture. The cells had undetectable levels of receptors for IL‐2, were not clonogenic in soft agar, but did form tumors in nude mice. Their establishment and continuous growth in vitro was dependent on the number of cells inoculated and on the growth medium used. Cytogenetic alteration, HTLV‐1 or reverse transcriptase activity were not detected. The production of known T‐cell derived lymphokines such as IL‐2, B‐cell growth factor(s), α‐interferon or granulocyte/macrophage colony stimulating or inhibiting factor(s) was not detected. The cells had 5–8% natural killer (NK)‐cell activity against NK‐cell sensitive target cells (K562) and were not sensitive for NK cells. A most unusual characteristic was the pronounced sensitivity of the cells to ascorbic acid. Concentrations down to 50 μmol/l killed the cells within hours.

Bibliography

Helgestad, J., Pettersen, R., StormâMathisen, I., Schjerven, L., Ulrich, K., Smeland, E. B., Egeland, T., SÃ¸rskaard, D., BrÃ¸gger, A., Hovig, T., DegrÃ©, M., & Lie, S. O. (1990). Characterization of a new malignant human Tâcell line (PFIâ285) sensitive to ascorbic acid. European Journal of Haematology, 44(1), 9â17. Portico.

Authors 12

Jon Helgestad (first)
Rolf Pettersen (additional)
Ingebørg Storm‐Mathisen (additional)
Leif Schjerven (additional)
Kay Ulrich (additional)
Erlend B. Smeland (additional)
Torstein Egeland (additional)
Dag Sørskaard (additional)
Anton Brøgger (additional)
Torstein Hovig (additional)
Miklos Degré (additional)
Sverre O. Lie (additional)

References 42 Referenced 13

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Dates

Type	When
Created	15 years, 1 month ago (July 17, 2010, 11:42 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 7:40 p.m.)
Indexed	1 year, 10 months ago (Oct. 26, 2023, 3:19 a.m.)
Issued	35 years, 7 months ago (Jan. 1, 1990)
Published	35 years, 7 months ago (Jan. 1, 1990)
Published Online	16 years, 4 months ago (April 24, 2009)
Published Print	35 years, 7 months ago (Jan. 1, 1990)

Funders 0

None

BibTeX

@article{Helgestad_1990, title={Characterization of a new malignant human T‐cell line (PFI‐285) sensitive to ascorbic acid}, volume={44}, ISSN={1600-0609}, url={http://dx.doi.org/10.1111/j.1600-0609.1990.tb00340.x}, DOI={10.1111/j.1600-0609.1990.tb00340.x}, number={1}, journal={European Journal of Haematology}, publisher={Wiley}, author={Helgestad, Jon and Pettersen, Rolf and Storm‐Mathisen, Ingebørg and Schjerven, Leif and Ulrich, Kay and Smeland, Erlend B. and Egeland, Torstein and Sørskaard, Dag and Brøgger, Anton and Hovig, Torstein and Degré, Miklos and Lie, Sverre O.}, year={1990}, month=jan, pages={9–17} }

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  "references-count": 42,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1990,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1600-0609.1990.tb00340.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1600-0609.1990.tb00340.x",
  "relation": {},
  "ISSN": [
    "0902-4441",
    "1600-0609"
  ],
  "subject": [],
  "container-title-short": "European J of Haematology",
  "published": {
    "date-parts": [
      [
        1990,
        1
      ]
    ]
  }
}