DOI: 10.1111/j.1550-7408.2001.tb00193.x. Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate <i>Euplotes octocarinatus</i>

Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate Euplotes octocarinatus

10.1111/j.1550-7408.2001.tb00193.x

Crossref journal-article

Wiley

Journal of Eukaryotic Microbiology (311)

Abstract

ABSTRACT. Three macronuclear genes encoding putative nuclear protein kinases of the ciliate Euplotes octocarinatus syngen I were isolated and sequenced. All three deduced gene products share significant properties with a group of recently identified nuclear serine/ threonine protein kinases named Ndr. The three predicted proteins contain the twelve conserved catalytic subdomains of protein kinases and 22 near universally‐conserved amino acids residues that are characteristic of serine/threonine protein kinases. In addition, there is an—30 amino acid‐peptide insertion between subdomains VII and VIII that contains a potential nuclear localization signal. Sequence analysis suggests that expression of the Eondr2 gene requires a +1 programmed translational frameshift for its translation. Comparison of the deduced EoNdr2 with other known Ndr protein kinases implies that a +1 ribosomal frameshift occurs at the motif AAATAA.

Bibliography

TAN, M., LIANG, A., BRÃNENâNIEWELER, C., & HECKMANN, K. (2001). Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate Euplotes octocarinatus. Journal of Eukaryotic Microbiology, 48(5), 575â582. Portico.

Authors 4

MING TAN (first)
AIHUA LIANG (additional)
CLAUDIA BRÜNEN‐NIEWELER (additional)
KLAUS HECKMANN (additional)

References 35 Referenced 17

10.1093/emboj/19.22.6230
10.1093/nar/25.17.3389
10.1016/0092-8674(90)90007-2
10.1016/0092-8674(90)90371-K
10.1093/nar/18.7.1725
10.1073/pnas.89.6.2262
10.1016/0022-2836(92)90901-U
10.1111/j.1365-2958.1993.tb01140.x
10.1016/0022-2836(88)90092-7
{'key': 'e_1_2_5_11_1', 'first-page': '235', 'article-title': 'Reduction of background problems in nonradioactive Northern and Southern blot analyses enables higher sensitivity than 32P‐based hybridizations. Anal', 'volume': '210', 'author': 'Engler‐Blum G.', 'year': '1993', 'journal-title': 'Biochem'} / Biochem / Reduction of background problems in nonradioactive Northern and Southern blot analyses enables higher sensitivity than 32P‐based hybridizations. Anal by Engler‐Blum G. (1993)
10.1128/mr.60.1.103-134.1996
10.1016/S0079-6603(00)64004-7
10.1016/0092-8674(93)90297-4
10.1073/pnas.87.10.3713
10.1146/annurev.bi.65.070196.003521
10.1126/science.1529352
10.1111/j.1550-7408.1996.tb05048.x
10.1016/0076-6879(91)00126-H
10.1093/emboj/19.8.1907
10.1038/331280a0
10.1016/0378-1119(93)90226-S
10.1006/jmbi.1997.1162
10.1016/0092-8674(95)90450-6
10.1073/pnas.88.9.3758
10.1073/pnas.92.11.5022
10.1074/jbc.274.48.33847
10.1093/emboj/17.20.5913
10.1128/mr.58.2.233-267.1994 / Microbiol. Rev / The DNA of ciliated protozoa by Prescott D. M. (1994)
10.1128/MCB.13.11.6931
10.1016/S0932-4739(99)80039-X
{'key': 'e_1_2_5_32_1', 'first-page': '80', 'article-title': 'Analysis of micronuclear, macronuclear and cDNA sequences encoding the regulatory subunit of cAMP‐dependent protein kinase of Euplotes octocarinatus: evidence for a ribosomal frameshift. J. Eukaryot', 'volume': '48', 'author': 'Tan M.', 'year': '2001', 'journal-title': 'Microbiol'} / Microbiol / Analysis of micronuclear, macronuclear and cDNA sequences encoding the regulatory subunit of cAMP‐dependent protein kinase of Euplotes octocarinatus: evidence for a ribosomal frameshift. J. Eukaryot by Tan M. (2001)
10.1093/nar/19.9.2457
10.1073/pnas.87.7.2516
10.1128/MCB.14.12.8107
{'key': 'e_1_2_5_36_1', 'first-page': '687', 'article-title': 'Slippery runs, shifty stops, backward steps, and forward hops: ‐2, ‐1, +1, +2, +5, and +6 ribosomal frameshifting. Cold Spring Harbor Symp. Quant', 'volume': '52', 'author': 'Weiss B. R.', 'year': '1987', 'journal-title': 'Biol'} / Biol / Slippery runs, shifty stops, backward steps, and forward hops: ‐2, ‐1, +1, +2, +5, and +6 ribosomal frameshifting. Cold Spring Harbor Symp. Quant by Weiss B. R. (1987)

Dates

Type	When
Created	20 years, 1 month ago (July 11, 2005, 2:10 p.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 8:59 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	23 years, 11 months ago (Sept. 1, 2001)
Published	23 years, 11 months ago (Sept. 1, 2001)
Published Online	20 years, 1 month ago (July 11, 2005)
Published Print	23 years, 11 months ago (Sept. 1, 2001)

Funders 0

None

BibTeX

@article{TAN_2001, title={Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate Euplotes octocarinatus}, volume={48}, ISSN={1550-7408}, url={http://dx.doi.org/10.1111/j.1550-7408.2001.tb00193.x}, DOI={10.1111/j.1550-7408.2001.tb00193.x}, number={5}, journal={Journal of Eukaryotic Microbiology}, publisher={Wiley}, author={TAN, MING and LIANG, AIHUA and BRÜNEN‐NIEWELER, CLAUDIA and HECKMANN, KLAUS}, year={2001}, month=sep, pages={575–582} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T18:32:23Z",
    "timestamp": 1751481143967
  },
  "reference-count": 35,
  "publisher": "Wiley",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            7,
            11
          ]
        ],
        "date-time": "2005-07-11T00:00:00Z",
        "timestamp": 1121040000000
      },
      "content-version": "vor",
      "delay-in-days": 1409,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        9
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>ABSTRACT. </jats:bold> Three macronuclear genes encoding putative nuclear protein kinases of the ciliate <jats:italic>Euplotes octocarinatus</jats:italic> syngen I were isolated and sequenced. All three deduced gene products share significant properties with a group of recently identified nuclear serine/ threonine protein kinases named Ndr. The three predicted proteins contain the twelve conserved catalytic subdomains of protein kinases and 22 near universally\u2010conserved amino acids residues that are characteristic of serine/threonine protein kinases. In addition, there is an\u201430 amino acid\u2010peptide insertion between subdomains VII and VIII that contains a potential nuclear localization signal. Sequence analysis suggests that expression of the <jats:italic>Eondr2</jats:italic> gene requires a +1 programmed translational frameshift for its translation. Comparison of the deduced EoNdr2 with other known Ndr protein kinases implies that a +1 ribosomal frameshift occurs at the motif AAATAA.</jats:p>",
  "DOI": "10.1111/j.1550-7408.2001.tb00193.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        7,
        11
      ]
    ],
    "date-time": "2005-07-11T18:10:17Z",
    "timestamp": 1121105417000
  },
  "page": "575-582",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 17,
  "title": "Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate <i>Euplotes octocarinatus</i>",
  "prefix": "10.1111",
  "volume": "48",
  "author": [
    {
      "given": "MING",
      "family": "TAN",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "AIHUA",
      "family": "LIANG",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "CLAUDIA",
      "family": "BR\u00dcNEN\u2010NIEWELER",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "KLAUS",
      "family": "HECKMANN",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        7,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_5_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.22.6230"
    },
    {
      "key": "e_1_2_5_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/25.17.3389"
    },
    {
      "key": "e_1_2_5_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90007-2"
    },
    {
      "key": "e_1_2_5_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90371-K"
    },
    {
      "key": "e_1_2_5_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/18.7.1725"
    },
    {
      "key": "e_1_2_5_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.6.2262"
    },
    {
      "key": "e_1_2_5_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(92)90901-U"
    },
    {
      "key": "e_1_2_5_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1993.tb01140.x"
    },
    {
      "key": "e_1_2_5_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90092-7"
    },
    {
      "key": "e_1_2_5_11_1",
      "first-page": "235",
      "article-title": "Reduction of background problems in nonradioactive Northern and Southern blot analyses enables higher sensitivity than 32P\u2010based hybridizations. Anal",
      "volume": "210",
      "author": "Engler\u2010Blum G.",
      "year": "1993",
      "journal-title": "Biochem"
    },
    {
      "key": "e_1_2_5_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mr.60.1.103-134.1996"
    },
    {
      "key": "e_1_2_5_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6603(00)64004-7"
    },
    {
      "key": "e_1_2_5_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90297-4"
    },
    {
      "key": "e_1_2_5_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.10.3713"
    },
    {
      "key": "e_1_2_5_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.65.070196.003521"
    },
    {
      "key": "e_1_2_5_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1529352"
    },
    {
      "key": "e_1_2_5_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1550-7408.1996.tb05048.x"
    },
    {
      "key": "e_1_2_5_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(91)00126-H"
    },
    {
      "key": "e_1_2_5_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.8.1907"
    },
    {
      "key": "e_1_2_5_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/331280a0"
    },
    {
      "key": "e_1_2_5_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(93)90226-S"
    },
    {
      "key": "e_1_2_5_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1997.1162"
    },
    {
      "key": "e_1_2_5_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90450-6"
    },
    {
      "key": "e_1_2_5_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.9.3758"
    },
    {
      "key": "e_1_2_5_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.11.5022"
    },
    {
      "key": "e_1_2_5_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.48.33847"
    },
    {
      "key": "e_1_2_5_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.20.5913"
    },
    {
      "key": "e_1_2_5_29_1",
      "doi-asserted-by": "crossref",
      "first-page": "223",
      "DOI": "10.1128/mr.58.2.233-267.1994",
      "article-title": "The DNA of ciliated protozoa",
      "volume": "58",
      "author": "Prescott D. M.",
      "year": "1994",
      "journal-title": "Microbiol. Rev"
    },
    {
      "key": "e_1_2_5_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.13.11.6931"
    },
    {
      "key": "e_1_2_5_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0932-4739(99)80039-X"
    },
    {
      "key": "e_1_2_5_32_1",
      "first-page": "80",
      "article-title": "Analysis of micronuclear, macronuclear and cDNA sequences encoding the regulatory subunit of cAMP\u2010dependent protein kinase of Euplotes octocarinatus: evidence for a ribosomal frameshift. J. Eukaryot",
      "volume": "48",
      "author": "Tan M.",
      "year": "2001",
      "journal-title": "Microbiol"
    },
    {
      "key": "e_1_2_5_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.9.2457"
    },
    {
      "key": "e_1_2_5_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.7.2516"
    },
    {
      "key": "e_1_2_5_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.14.12.8107"
    },
    {
      "key": "e_1_2_5_36_1",
      "first-page": "687",
      "article-title": "Slippery runs, shifty stops, backward steps, and forward hops: \u20102, \u20101, +1, +2, +5, and +6 ribosomal frameshifting. Cold Spring Harbor Symp. Quant",
      "volume": "52",
      "author": "Weiss B. R.",
      "year": "1987",
      "journal-title": "Biol"
    }
  ],
  "container-title": "Journal of Eukaryotic Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1550-7408.2001.tb00193.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1550-7408.2001.tb00193.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        15
      ]
    ],
    "date-time": "2023-10-15T00:59:29Z",
    "timestamp": 1697331569000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1550-7408.2001.tb00193.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        9
      ]
    ]
  },
  "references-count": 35,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2001,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1550-7408.2001.tb00193.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1550-7408.2001.tb00193.x",
  "relation": {},
  "ISSN": [
    "1066-5234",
    "1550-7408"
  ],
  "subject": [],
  "container-title-short": "J Eukaryotic Microbiology",
  "published": {
    "date-parts": [
      [
        2001,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "2005-07-11",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}