DOI: 10.1111/j.1550-7408.1968.tb02097.x. Behavior of the Kinetoplast of <i>Leishmania tarentolae</i> upon Cell Rupture*

Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*

10.1111/j.1550-7408.1968.tb02097.x

Crossref journal-article

Wiley

The Journal of Protozoology (311)

Abstract

SYNOPSIS. The kinetoplast of L. tarentolae remains attached to the basal body upon cell rupture by detergent lysis, sonication, or hypotonic lysis in 0.02 M Tris buffer (pH 7.9) at 0–4 C. Hypotonic lysis in 0.02 M Tris‐HCl‐2 mM EDTA at 0–4 C and application of mild shearing forces bring about release of most of the swollen kinetoplasts. The kinetoplast DNA can be seen in phase contrast microscopy as a dark mass contiguous to the kinetoplast membrane directly opposite the basal body. Upon return to isotonic media, the kinetoplast shrinks; the membranes of such kinetoplasts are impermeable to added DNAase.

Bibliography

SIMPSON, L. (1968). Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*. The Journal of Protozoology, 15(1), 132â136. Portico.

Authors 1

LARRY SIMPSON (first)

References 26 Referenced 18

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10.1111/j.1550-7408.1960.tb00717.x
{'key': 'e_1_2_1_6_2', 'first-page': '813', 'article-title': 'The kinetoplast in Crithidia fasciculata', 'volume': '120', 'author': 'Cosgrove W. H.', 'year': '1954', 'journal-title': 'Anat. Rec.'} / Anat. Rec. / The kinetoplast in Crithidia fasciculata by Cosgrove W. H. (1954)
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{'key': 'e_1_2_1_10_2', 'first-page': '194', 'article-title': 'Study of Leishmania donovani with special reference to the kinetoplast, mitochondria and golgi zone by electron microscopy employing the thin section technique', 'volume': '1', 'author': 'Inoki S.', 'year': '1958', 'journal-title': "Biken's J."} / Biken's J. / Study of Leishmania donovani with special reference to the kinetoplast, mitochondria and golgi zone by electron microscopy employing the thin section technique by Inoki S. (1958)
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Dates

Type	When
Created	18 years, 3 months ago (May 13, 2007, 8:27 p.m.)
Deposited	1 year, 10 months ago (Nov. 1, 2023, 2:35 p.m.)
Indexed	1 year, 10 months ago (Nov. 1, 2023, 9:11 p.m.)
Issued	57 years, 7 months ago (Feb. 1, 1968)
Published	57 years, 7 months ago (Feb. 1, 1968)
Published Online	18 years, 4 months ago (April 30, 2007)
Published Print	57 years, 7 months ago (Feb. 1, 1968)

Funders 0

None

BibTeX

@article{SIMPSON_1968, title={Behavior of the Kinetoplast of Leishmania tarentolae upon Cell Rupture*}, volume={15}, ISSN={0022-3921}, url={http://dx.doi.org/10.1111/j.1550-7408.1968.tb02097.x}, DOI={10.1111/j.1550-7408.1968.tb02097.x}, number={1}, journal={The Journal of Protozoology}, publisher={Wiley}, author={SIMPSON, LARRY}, year={1968}, month=feb, pages={132–136} }

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