DOI: 10.1111/j.1476-5381.1995.tb13341.x. Release by ultraviolet B (u.v.B) radiation of nitric oxide (NO) from human keratinocytes: a potential role for nitric oxide in erythema production

Release by ultraviolet B (u.v.B) radiation of nitric oxide (NO) from human keratinocytes: a potential role for nitric oxide in erythema production

10.1111/j.1476-5381.1995.tb13341.x

Crossref journal-article

Wiley

British Journal of Pharmacology (311)

Abstract

1 The mechanism of human sunburn is poorly understood but its characteristic features include the development of erythema. In this study we attempted to determine whether human keratinocytes possess a nitric oxide (NO) synthase (NOS), if this enzyme could be activated to release NO following exposure to ultraviolet B (u.v.B) and to define whether this photo‐induced response could be involved in the pathogenesis of sunburn erythema.2 Treatment of human keratinocytes with various doses of u.v.B (290–320 nm) radiation (up to 100 mJ cm−2) resulted in a dose‐dependent release of NO and cyclic GMP production that was reduced by NG‐monomethyl‐l‐arginine (l‐NMMA).3 u.v.B irradiation of keratinocyte cytosol at varying doses (up to 50 mJ cm−2), resulted in a gradual rise in NO production, with a concomitant increase in soluble guanylate cyclase activity (sGC).4 NOS isolated from the keratinocyte cytosol was constitutively expressed and was dependent on NADPH, Ca2+/calmodulin, tetrahydrobiopterin and flavins.5 In reconstitution experiments, when purified NOS was added to purified sGC, both isolated from keratinocyte cytosol, a four fold increase in cyclic GMP was observed. The GMP was increased by NO synthesized following u.v.B radiation (up to 20 mJ cm−2) of NOS.6 In in vivo experiments, guinea‐pigs were subjected to u.v.B light. A Protection Factor (PF) of 8.71 ± 2.85 was calculated when an emulsified cream formulation containing l‐NMMA (2%) was applied to their skin.7 The present results indicate that u.v.B radiation acts as a potent stimulator of NOS in keratinocytes. NO is lipophilic and may diffuse out of the keratinocytes, activating sGC in endothelial cells and neighbouring smooth muscle cells. This may be a major part of the integrated response of the skin leading to vasodilatation and erythema.

Bibliography

Deliconstantinos, G., Villiotou, V., & Stravrides, J. C. (1995). Release by ultraviolet B (u.v.B) radiation of nitric oxide (NO) from human keratinocytes: a potential role for nitric oxide in erythema production. British Journal of Pharmacology, 114(6), 1257â1265. Portico.

Authors 3

George Deliconstantinos (first)
Vassiliki Villiotou (additional)
John C. Stravrides (additional)

References 33 Referenced 94

10.1016/0024-3205(94)00578-8
10.1016/0003-2697(76)90527-3
10.1073/pnas.87.2.682
10.1016/0014-5793(90)80902-U
10.1016/S0733-8635(18)30820-9
{'key': 'e_1_2_1_7_1', 'first-page': '182', 'volume-title': 'Biology of Nitric Oxide', 'author': 'DELICONSTANTINOS G.', 'year': '1992'} / Biology of Nitric Oxide by DELICONSTANTINOS G. (1992)
10.1097/00005344-199204002-00019
{'key': 'e_1_2_1_9_1', 'first-page': '101', 'volume-title': 'Liver Transplantation in Children', 'author': 'DELICONSTANTINOS G.', 'year': '1993'} / Liver Transplantation in Children by DELICONSTANTINOS G. (1993)
{'key': 'e_1_2_1_10_1', 'first-page': '77', 'article-title': 'Pathophysiology of nitric oxide in cancer', 'volume': '1', 'author': 'DELICONSTANTINOS G.', 'year': '1994', 'journal-title': 'Cancer Mol. Biol.'} / Cancer Mol. Biol. / Pathophysiology of nitric oxide in cancer by DELICONSTANTINOS G. (1994)
{'key': 'e_1_2_1_11_1', 'first-page': '401', 'volume-title': 'Vasodilatation: Vascular Smooth Muscle, Peptides, Autonomic Nerves and Endothelium', 'author': 'FURCHGOTT R.F.', 'year': '1988'} / Vasodilatation: Vascular Smooth Muscle, Peptides, Autonomic Nerves and Endothelium by FURCHGOTT R.F. (1988)
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10.1016/S0021-9258(19)36601-3 / J. Biol. Chem. / Epidermal growth factor suppresses nitric oxide and hydrogen peroxide production by keratinocytes by HECK D.E. (1992)
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10.1111/j.1600-0773.1990.tb00772.x
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{'key': 'e_1_2_1_21_1', 'first-page': '109', 'article-title': 'Nitric oxide: physiology, pathophysiology and pharmacology', 'volume': '43', 'author': 'MONCADA S.', 'year': '1991', 'journal-title': 'Pharmacol. Rev.'} / Pharmacol. Rev. / Nitric oxide: physiology, pathophysiology and pharmacology by MONCADA S. (1991)
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10.1038/327524a0
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10.1016/0167-4889(93)90006-B
10.1042/bj2810297 / Biochem. J. / Tetrahydrobiopterin‐dependent formation of endothelium‐derived relaxing factor (nitric oxide) in aortic endothelial cells by SCHMIDT K. (1992)
10.1016/0167-4889(86)90144-8
10.1111/j.1476-5381.1987.tb11347.x
10.1016/0090-6980(76)90066-6
10.1126/science.1281928
10.1111/j.1476-5381.1993.tb13645.x
10.1021/ac50061a029

Dates

Type	When
Created	13 years, 1 month ago (July 20, 2012, 10:24 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 4:47 a.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:27 a.m.)
Issued	30 years, 5 months ago (March 1, 1995)
Published	30 years, 5 months ago (March 1, 1995)
Published Online	13 years, 1 month ago (July 19, 2012)
Published Print	30 years, 5 months ago (March 1, 1995)

Funders 0

None

BibTeX

@article{Deliconstantinos_1995, title={Release by ultraviolet B (u.v.B) radiation of nitric oxide (NO) from human keratinocytes: a potential role for nitric oxide in erythema production}, volume={114}, ISSN={1476-5381}, url={http://dx.doi.org/10.1111/j.1476-5381.1995.tb13341.x}, DOI={10.1111/j.1476-5381.1995.tb13341.x}, number={6}, journal={British Journal of Pharmacology}, publisher={Wiley}, author={Deliconstantinos, George and Villiotou, Vassiliki and Stravrides, John C.}, year={1995}, month=mar, pages={1257–1265} }

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