DOI: 10.1111/j.1476-5381.1984.tb10132.x. Evidence that ethylenediamine acts in the isolated ileum of the guinea‐pig by releasing endogenous GABA

Evidence that ethylenediamine acts in the isolated ileum of the guinea‐pig by releasing endogenous GABA

10.1111/j.1476-5381.1984.tb10132.x

Crossref journal-article

Wiley

British Journal of Pharmacology (311)

Abstract

Ethylenediamine (EDA) released [3H]‐γ‐aminobutyric acid ([3H]‐GABA) in a dose‐dependent manner from the isolated preloaded ileum of the guinea‐pig maintained in Krebs‐bicarbonate solution (pH 7.4, 37°C), in the presence of β‐alanine and amino‐oxyacetic acid (AOAA) to prevent GABA uptake into glial cells and catabolism. This release was reversibly prevented by 3‐mercaptopropionic acid (3‐MPA), also in a dose‐dependent manner. In the isolated ileal preparations of the guinea‐pig maintained in Krebs‐bicarbonate solution, EDA induced a dose‐dependent transient, cholinergic contractile response (GABAA‐receptor‐mediated effect), followed by an ‘after‐relaxation’ (GABAB‐receptor‐mediated effect). EDA also induced a transient contraction superimposed on repetitive twitch responses to electrical transmural stimulation of the cholinergic neurones, followed by a depression of the twitch contractions. This GABAA‐receptor‐mediated contraction was antagonized by bicuculline methochloride and picrotoxinin, whilst the GABAB‐receptor‐mediated ‘after‐relaxation’, and depression of cholinergic twitch contractions, was susceptible to antagonism by δ‐aminovaleric acid. The pA2 value for bicuculline methochloride antagonism of EDA was estimated to be 5.8, identical with that for GABA. 3‐Mercaptopropionic acid also prevented these pharmacological actions induced by EDA without affecting responses to GABA, 3‐aminopropranesulphonic acid, muscimol, baclofen or the twitch responses to transmural stimulation. It is concluded that EDA releases both [3H]‐GABA and endogenous GABA in the guinea‐pig ileum, thus providing further evidence that GABA is a transmitter in the enteric nervous system.

Bibliography

Kerr, D. I. B., & Ong, J. (1984). Evidence that ethylenediamine acts in the isolated ileum of the guineaâpig by releasing endogenous GABA. British Journal of Pharmacology, 83(1), 169â177. Portico.

Authors 2

D.I.B. Kerr (first)
Jennifer Ong (additional)

References 25 Referenced 30

10.1016/0014-2999(81)90386-1
10.1016/0197-0186(83)90009-8
10.1016/0006-8993(81)91001-5
{'key': 'e_1_2_1_5_1', 'first-page': '274P', 'article-title': 'Ethylenediamine acts upon GABA receptors and uptake sites', 'volume': '74', 'author': 'Forster P.', 'year': '1981', 'journal-title': 'Br. J. Pharmac.'} / Br. J. Pharmac. / Ethylenediamine acts upon GABA receptors and uptake sites by Forster P. (1981)
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{'key': 'e_1_2_1_7_1', 'first-page': '406', 'article-title': 'Putative neurotransmitters; criteria for establishing a neurotransmitter', 'volume': '17', 'author': 'Iversen L.L.', 'year': '1979', 'journal-title': 'Neurosci. Res. Prog. Bull.'} / Neurosci. Res. Prog. Bull. / Putative neurotransmitters; criteria for establishing a neurotransmitter by Iversen L.L. (1979)
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{'key': 'e_1_2_1_13_1', 'first-page': '453', 'article-title': 'GABA is a transmitter in the enteric nervous system', 'volume': '15', 'author': 'Kerr D.I.B.', 'year': '1983', 'journal-title': 'Internat. Union Physiol. Sci. 29th Congress Proc.'} / Internat. Union Physiol. Sci. 29th Congress Proc. / GABA is a transmitter in the enteric nervous system by Kerr D.I.B. (1983)
{'key': 'e_1_2_1_14_1', 'first-page': '390P', 'article-title': 'GABA actions in guinea‐pig ileum through release of endogenous GABA by ethylenediamine', 'volume': '9', 'author': 'Kerr D.I.B.', 'year': '1982', 'journal-title': 'Clin. exp. Pharmac. Physiol.'} / Clin. exp. Pharmac. Physiol. / GABA actions in guinea‐pig ileum through release of endogenous GABA by ethylenediamine by Kerr D.I.B. (1982)
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10.1111/j.1471-4159.1982.tb05366.x
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10.1016/0014-2999(82)90390-9
10.1016/0304-3940(81)90019-7
10.1126/science.7112110

Dates

Type	When
Created	13 years, 1 month ago (July 20, 2012, 4:56 a.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 11:13 p.m.)
Indexed	1 year, 10 months ago (Oct. 24, 2023, 3:57 a.m.)
Issued	41 years ago (Sept. 1, 1984)
Published	41 years ago (Sept. 1, 1984)
Published Online	13 years, 1 month ago (July 19, 2012)
Published Print	41 years ago (Sept. 1, 1984)

Funders 0

None

BibTeX

@article{Kerr_1984, title={Evidence that ethylenediamine acts in the isolated ileum of the guinea‐pig by releasing endogenous GABA}, volume={83}, ISSN={1476-5381}, url={http://dx.doi.org/10.1111/j.1476-5381.1984.tb10132.x}, DOI={10.1111/j.1476-5381.1984.tb10132.x}, number={1}, journal={British Journal of Pharmacology}, publisher={Wiley}, author={Kerr, D.I.B. and Ong, Jennifer}, year={1984}, month=sep, pages={169–177} }

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