DOI: 10.1111/j.1476-5381.1974.tb09717.x. THE RELEASE OF LABELLED ACETYLCHOLINE AND CHOLINE FROM CEREBRAL CORTICAL SLICES STIMULATED ELECTRICALLY

THE RELEASE OF LABELLED ACETYLCHOLINE AND CHOLINE FROM CEREBRAL CORTICAL SLICES STIMULATED ELECTRICALLY

10.1111/j.1476-5381.1974.tb09717.x

Crossref journal-article

Wiley

British Journal of Pharmacology (311)

Abstract

In order to establish the origin of the increased efflux of radioactivity caused by electrical stimulation of cerebral cortical slices which had been incubated with [3H]‐choline, labelled choline and acetylcholine (ACh) collected by superfusion were separated by gold precipitation. In the presence of physostigmine electrical stimulation (1 Hz, 10 min) increased the release of only [3H]‐ACh which was greatly enhanced by the addition of atropine. Continuous stimulation in the presence of physostigmine resulted in an evoked release of [3H]‐ACh which declined asymptotically. This evoked release appeared to follow first‐order kinetics with a rate constant which remained stable over the course of prolonged stimulation. The rate constant for the evoked release of [3H]‐ACh with 1 Hz stimulation was three times greater in the presence of physostigmine and atropine than in the presence of physostigmine alone, while the size of the store from which [3H]‐ACh was released was nearly identical under these two conditions. In the absence of physostigmine and atropine, stimulation caused the appearance of only [3H]‐choline in the samples. Reduction of [3H]‐ACh stores before the application of physostigmine resulted in a reduced evoked release of total radioactivity, both in the absence or presence of physostigmine and atropine, and decreased the evoked release of [3H]‐ACh without affecting the release of [3H]‐choline. Results suggest that electrical stimulation of cortical slices which had been incubated with [3H]‐choline causes the release of only [3H]‐ACh, both in the presence or absence of an anticholinesterase. The evoked increase in the efflux of total radioactivity is therefore a good measure of the release of [3H]‐ACh.

Bibliography

RICHARDSON, I. W., & SZERB, J. C. (1974). THE RELEASE OF LABELLED ACETYLCHOLINE AND CHOLINE FROM CEREBRAL CORTICAL SLICES STIMULATED ELECTRICALLY. British Journal of Pharmacology, 52(4), 499â507. Portico.

Authors 2

I.W. RICHARDSON (first)
J.C. SZERB (additional)

References 13 Referenced 131

Dates

Type	When
Created	13 years, 1 month ago (July 19, 2012, 1:52 p.m.)
Deposited	1 year, 10 months ago (Nov. 4, 2023, 7:13 a.m.)
Indexed	1 year, 1 month ago (July 12, 2024, 5:51 a.m.)
Issued	50 years, 9 months ago (Dec. 1, 1974)
Published	50 years, 9 months ago (Dec. 1, 1974)
Published Online	13 years, 1 month ago (July 19, 2012)
Published Print	50 years, 9 months ago (Dec. 1, 1974)

Funders 0

None

BibTeX

@article{RICHARDSON_1974, title={THE RELEASE OF LABELLED ACETYLCHOLINE AND CHOLINE FROM CEREBRAL CORTICAL SLICES STIMULATED ELECTRICALLY}, volume={52}, ISSN={1476-5381}, url={http://dx.doi.org/10.1111/j.1476-5381.1974.tb09717.x}, DOI={10.1111/j.1476-5381.1974.tb09717.x}, number={4}, journal={British Journal of Pharmacology}, publisher={Wiley}, author={RICHARDSON, I.W. and SZERB, J.C.}, year={1974}, month=dec, pages={499–507} }

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