DOI: 10.1111/j.1471-4159.2011.07255.x. Rapid, complete and large‐scale generation of post‐mitotic neurons from the human LUHMES cell line

Rapid, complete and large‐scale generation of post‐mitotic neurons from the human LUHMES cell line

10.1111/j.1471-4159.2011.07255.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

J. Neurochem. (2011) 119, 957–971.AbstractWe characterized phenotype and function of a fetal human mesencephalic cell line (LUHMES, Lund human mesencephalic) as neuronal model system. Neurodevelopmental profiling of the proliferation stage (d0, day 0) of these conditionally‐immortalized cells revealed neuronal features, expressed simultaneously with some early neuroblast and stem cell markers. An optimized 2‐step differentiation procedure, triggered by shut‐down of the myc transgene, resulted in uniformly post‐mitotic neurons within 5 days (d5). This was associated with down‐regulation of some precursor markers and further up‐regulation of neuronal genes. Neurite network formation involved the outgrowth of 1–2, often > 500 μm long projections. They showed dynamic growth cone behavior, as evidenced by time‐lapse imaging of stably GFP‐over‐expressing cells. Voltage‐dependent sodium channels and spontaneous electrical activity of LUHMES continuously increased from d0 to d11, while levels of synaptic markers reached their maximum on d5. The developmental expression patterns of most genes and of the dopamine uptake‐ and release‐machinery appeared to be intrinsically predetermined, as the differentiation proceeded similarly when external factors such as dibutyryl‐cAMP and glial cell derived neurotrophic factor were omitted. Only tyrosine hydroxylase required the continuous presence of cAMP. In conclusion, LUHMES are a robust neuronal model with adaptable phenotype and high value for neurodevelopmental studies, disease modeling and neuropharmacology.

Bibliography

Scholz, D., PÃ¶ltl, D., Genewsky, A., Weng, M., Waldmann, T., Schildknecht, S., & Leist, M. (2011). Rapid, complete and largeâscale generation of postâmitotic neurons from the human LUHMES cell line. Journal of Neurochemistry, 119(5), 957â971. Portico.

Authors 7

Diana Scholz (first)
Dominik Pöltl (additional)
Andreas Genewsky (additional)
Matthias Weng (additional)
Tanja Waldmann (additional)
Stefan Schildknecht (additional)
Marcel Leist (additional)

References 46 Referenced 271

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Dates

Type	When
Created	14 years, 5 months ago (March 25, 2011, 5:49 a.m.)
Deposited	10 months, 1 week ago (Oct. 14, 2024, 1:18 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 8:59 a.m.)
Issued	14 years, 4 months ago (April 13, 2011)
Published	14 years, 4 months ago (April 13, 2011)
Published Online	14 years, 4 months ago (April 13, 2011)
Published Print	13 years, 8 months ago (Dec. 1, 2011)

Funders 0

None

BibTeX

@article{Scholz_2011, title={Rapid, complete and large‐scale generation of post‐mitotic neurons from the human LUHMES cell line}, volume={119}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.2011.07255.x}, DOI={10.1111/j.1471-4159.2011.07255.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Scholz, Diana and Pöltl, Dominik and Genewsky, Andreas and Weng, Matthias and Waldmann, Tanja and Schildknecht, Stefan and Leist, Marcel}, year={2011}, month=apr, pages={957–971} }

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