DOI: 10.1111/j.1471-4159.2005.03560.x. Brain‐type creatine kinase activates neuron‐specific K<sup>+</sup>‐Cl<sup>

Brain‐type creatine kinase activates neuron‐specific K+‐Cl– co‐transporter KCC2

10.1111/j.1471-4159.2005.03560.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractGABA, a major inhibitory neurotransmitter in the adult CNS, is excitatory at early developmental stages as a result of the elevated intracellular Cl– concentration ([Cl–]i). This functional switch is primarily attributable to the K+‐Cl– co‐transporter KCC2, the expression of which is developmentally regulated in neurons. Previously, we reported that KCC2 interacts with brain‐type creatine kinase (CKB). To elucidate the functional significance of this interaction, HEK293 cells were transfected with KCC2 and glycine receptor α2 subunit, and gramicidin‐perforated patch‐clamp recordings were performed to measure the glycine reversal potential (Egly), giving an estimate of [Cl–]i. KCC2‐expressing cells displayed the expected changes in Egly following alterations in the extracellular K+ concentration ([K+]o) or administration of an inhibitor of KCCs, suggesting that the KCC2 function was being properly assessed. When added into KCC2‐expressing cells, dominant‐negative CKB induced a depolarizing shift in Egly and reduced the hyperpolarizing shift in Egly seen in response to a lowering of [K+]o compared with wild‐type CKB. Moreover, 2,4‐dinitrofluorobenzene (DNFB), an inhibitor of CKs, shifted Egly in the depolarizing direction. In primary cortical neurons expressing CKB, the GABA reversal potential was also shifted in the depolarizing direction by DNFB. Our findings suggest that, in the cellular micro‐environment, CKB activates the KCC2 function.

Bibliography

Inoue, K., Yamada, J., Ueno, S., & Fukuda, A. (2005). Brainâtype creatine kinase activates neuronâspecific K+âClâ coâtransporter KCC2. Journal of Neurochemistry, 96(2), 598â608. Portico.

Authors 4

Koichi Inoue (first)
Junko Yamada (additional)
Shinya Ueno (additional)
Atsuo Fukuda (additional)

References 51 Referenced 61

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Dates

Type	When
Created	19 years, 9 months ago (Nov. 29, 2005, 6:14 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 10:46 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 9:51 p.m.)
Issued	19 years, 9 months ago (Nov. 29, 2005)
Published	19 years, 9 months ago (Nov. 29, 2005)
Published Online	19 years, 9 months ago (Nov. 29, 2005)
Published Print	19 years, 7 months ago (Jan. 1, 2006)

Funders 0

None

BibTeX

@article{Inoue_2005, title={Brain‐type creatine kinase activates neuron‐specific K+‐Cl– co‐transporter KCC2}, volume={96}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.2005.03560.x}, DOI={10.1111/j.1471-4159.2005.03560.x}, number={2}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Inoue, Koichi and Yamada, Junko and Ueno, Shinya and Fukuda, Atsuo}, year={2005}, month=nov, pages={598–608} }

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