DOI: 10.1111/j.1471-4159.2004.02345.x. Reticulon 1‐C/neuroendocrine‐specific protein‐C interacts with SNARE proteins

Reticulon 1‐C/neuroendocrine‐specific protein‐C interacts with SNARE proteins

10.1111/j.1471-4159.2004.02345.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractReticulons are proteins of neuroendocrine cells localized primarily to the endoplasmic reticulum membrane. Despite their implication in cellular processes like apoptosis or axonal regeneration, their intracellular molecular function is still largely unknown. Here, we show that reticulon 1‐C can be detected in a protein complex of 150–200 kDa, and that a number of soluble N‐ethylmaleimide‐sensitive factor attachment protein receptor (SNARE) proteins, i.e. syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2, can be co‐immunoprecipitated with reticulon 1‐C. Moreover, it localizes to a nocodazole‐sensitive, but calreticulin‐negative domain of the endoplasmic reticulum. Finally, overexpression in PC12 cells of a reticulon 1‐C fragment which binds to SNAREs, significantly enhances human growth hormone secretion. These results suggest that reticulons are involved in vesicle trafficking events, including regulated exocytosis.

Bibliography

Steiner, P., Kulangara, K., Sarria, J. C. F., Glauser, L., Regazzi, R., & Hirling, H. (2004). Reticulon 1âC/neuroendocrineâspecific proteinâC interacts with SNARE proteins. Journal of Neurochemistry, 89(3), 569â580. Portico.

Authors 6

Pascal Steiner (first)
Karina Kulangara (additional)
J. C. Floyd Sarria (additional)
Liliane Glauser (additional)
Romano Regazzi (additional)
Harald Hirling (additional)

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Dates

Type	When
Created	19 years, 8 months ago (Dec. 1, 2005, 11:49 a.m.)
Deposited	1 year, 10 months ago (Oct. 15, 2023, 1:47 a.m.)
Indexed	4 months, 1 week ago (April 15, 2025, 5:29 a.m.)
Issued	21 years, 5 months ago (March 22, 2004)
Published	21 years, 5 months ago (March 22, 2004)
Published Online	21 years, 5 months ago (March 22, 2004)
Published Print	21 years, 3 months ago (May 1, 2004)

Funders 0

None

BibTeX

@article{Steiner_2004, title={Reticulon 1‐C/neuroendocrine‐specific protein‐C interacts with SNARE proteins}, volume={89}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.2004.02345.x}, DOI={10.1111/j.1471-4159.2004.02345.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Steiner, Pascal and Kulangara, Karina and Sarria, J. C. Floyd and Glauser, Liliane and Regazzi, Romano and Hirling, Harald}, year={2004}, month=mar, pages={569–580} }

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