DOI: 10.1111/j.1471-4159.1993.tb13649.x. B‐50/GAP‐43 Binds to Actin Filaments Without Affecting Actin Polymerization and Filament Organization

B‐50/GAP‐43 Binds to Actin Filaments Without Affecting Actin Polymerization and Filament Organization

10.1111/j.1471-4159.1993.tb13649.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: To investigate a possible function of the nervous tissuespecific protein kinase C substrate B‐50/GAP‐43 in regulati of the dynamics of the submembranous cytoskeleton. we studii the interaction between purified 6–50 and actin. Both the phosphorylated and dephosphorylated forms of 8–50 cosedi‐mented with filamentous actin (F‐actin) in a Ca2+‐independent manner. Neither 6–50 nor phospho‐6–50 had any effect on the kinetics of actin polymerization and on the critical concentration at steady state, as measured using pyrenylated actin. tight scattering of F‐actin samples was not increased in the presence of 550, suggesting that 550 does not bundle actin filaments. The number of actin filaments, determined by [3H]cytochalasin B binding, was not affected by either phospho‐ or dephospho‐B‐50, indicating that 550 has neither a severing nor a capping effect. These observations were confirmed by electron microscopic evaluation of negatively stained F‐actin samples, which did not reveal any structural changes in the actin meshwork on addition of 6–50, We conclude that 6–50 is an actin‐binding protein that does not directly affect actin dynamics.

Bibliography

Hens, J. J. H., Fabio Benfenati, Nielander, H. B., Valtorta, F., Gispen, W. H., & De Graan, P. N. E. (1993). Bâ50/GAPâ43 Binds to Actin Filaments Without Affecting Actin Polymerization and Filament Organization. Journal of Neurochemistry, 61(4), 1530â1533. Portico.

Authors 6

Jacques J. H. Hens (first)
Fabio Benfenati (additional)
Henk B. Nielander (additional)
Flavia Valtorta (additional)
Willem Hendnk Gispen (additional)
Pierre N. E. De Graan (additional)

References 26 Referenced 37

{'key': 'e_1_2_2_2_1', 'first-page': '2321', 'article-title': 'The MARCKS brothers: a family of protein kinase C substrates. Cell 71, 713–716. Ando T. and Scales D. (1985) Skeletal muscle myosin subfragment‐1 induces bundle formation bv actin filaments', 'volume': '260', 'author': 'Aderem A.', 'year': '1992', 'journal-title': 'J. Biol. Chem'} / J. Biol. Chem / The MARCKS brothers: a family of protein kinase C substrates. Cell 71, 713–716. Ando T. and Scales D. (1985) Skeletal muscle myosin subfragment‐1 induces bundle formation bv actin filaments by Aderem A. (1992)
10.1038/326704a0
{'key': 'e_1_2_2_4_1', 'first-page': '121', 'volume-title': 'The Nmronul Cytoskeleton', 'author': 'Bamburg J. R.', 'year': '1991'} / The Nmronul Cytoskeleton by Bamburg J. R. (1991)
{'key': 'e_1_2_2_5_1', 'first-page': '377', 'article-title': 'Interaction of free and synaptic vesicle‐bound synapsin I with F‐actin', 'volume': '8', 'author': 'Benfenati F.', 'year': '1992', 'journal-title': 'Neicron'} / Neicron / Interaction of free and synaptic vesicle‐bound synapsin I with F‐actin by Benfenati F. (1992)
10.1016/0896-6273(89)90039-1
10.1016/S0021-9258(18)53878-3 / J. Biol. Chem. / The MARCKS family of cellular protein kinase C substrates by Blackshear P. J. (1993)
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10.1002/neu.480230506
10.1016/S0021-9258(19)49908-0 / J. Biol. Chetn. / Effects of the neuronal phosphoprotein synapsin I on actin polymerization. I. Evidence for a phosphorylationdependent nucleating effect by Valtorta F. (1992)
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10.1083/jcb.120.2.503

Dates

Type	When
Created	18 years, 8 months ago (Dec. 15, 2006, 9:15 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 10:43 p.m.)
Indexed	1 year, 3 months ago (May 28, 2024, 4:52 p.m.)
Issued	31 years, 11 months ago (Oct. 1, 1993)
Published	31 years, 11 months ago (Oct. 1, 1993)
Published Online	18 years, 8 months ago (Dec. 15, 2006)
Published Print	31 years, 11 months ago (Oct. 1, 1993)

Funders 0

None

BibTeX

@article{Hens_1993, title={B‐50/GAP‐43 Binds to Actin Filaments Without Affecting Actin Polymerization and Filament Organization}, volume={61}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1993.tb13649.x}, DOI={10.1111/j.1471-4159.1993.tb13649.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Hens, Jacques J. H. and Fabio Benfenati and Nielander, Henk B. and Valtorta, Flavia and Gispen, Willem Hendnk and De Graan, Pierre N. E.}, year={1993}, month=oct, pages={1530–1533} }

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