DOI: 10.1111/j.1471-4159.1993.tb03594.x. Evaluation of RNAs Present in Synaptodendrosomes: Dendritic, Glial, and Neuronal Cell Body Contribution

Evaluation of RNAs Present in Synaptodendrosomes: Dendritic, Glial, and Neuronal Cell Body Contribution

10.1111/j.1471-4159.1993.tb03594.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: “Synaptodendrosomes” are subcellular fractions that contain pinched‐off axon terminals and dendrites. These fractions are a potential source of RNAs that are localized in dendrites. However, these fractions may also contain RNAs that are seen in situ in neuronal cell bodies and glia. To evaluate whether Synaptodendrosomes could be used as a source of dendritic RNA, we studied the RNA content of this fraction as compared with RNA isolated from total forebrain and a cell body‐enriched fraction. RNA was analyzed by gel electrophoresis, oligo‐dT chromatography, and northern blot hybridization. RNA from Synaptodendrosomes contained a greater proportion of low‐molecular‐weight nonpolyadenylated RNAs. RNAs known to be present in dendrites (mRNA for the a subunit of the calcium/calmodulin‐dependent protein kinase II and the polymerase III transcript BC1) were detected in Synaptodendrosomes; RNAs that are restricted to neuronal and glial cell bodies (mRNAs for the 68‐kDa neurofilament protein, 43‐kDa growth‐associated protein, β‐tubulin, and β‐ actin) were present only at low levels. However, the mRNA for glial fibrillary acidic protein (seen in situ in glial cell bodies and processes) was present at high levels in the synap‐ todendrosomes. These results support and extend previous studies indicating that a limited subset of mRNAs is present in neuronal and astrocyte processes and reveal that both of these types of mRNAs are present in Synaptodendrosomes. Thus, Synaptodendrosomes may be useful as a source of dendritic RNAs, but it will be necessary to develop strategies to subtract mRNAs present in astroglial processes.

Bibliography

Rao, A., & Steward, O. (1993). Evaluation of RNAs Present in Synaptodendrosomes: Dendritic, Glial, and Neuronal Cell Body Contribution. Journal of Neurochemistry, 61(3), 835â844. Portico.

Authors 2

Anuradha Rao (first)
Oswald Steward (additional)

References 36 Referenced 43

10.1021/bi00847a025
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10.1523/JNEUROSCI.11-01-00031.1991
10.1016/0306-4522(92)90188-8
10.1016/0896-6273(90)90340-L
10.1523/JNEUROSCI.10-06-01788.1990
{'key': 'e_1_2_2_8_1', 'first-page': '379', 'article-title': 'mRNA at the synapse: analysis of a preparation enriched in hippocampal dendritic spine mRNA', 'volume': '17', 'author': 'Chicurel M. E.', 'year': '1991', 'journal-title': 'Soc. Neurosci. Abstr.'} / Soc. Neurosci. Abstr. / mRNA at the synapse: analysis of a preparation enriched in hippocampal dendritic spine mRNA by Chicurel M. E. (1991)
10.1523/JNEUROSCI.13-09-04054.1993 / J. Neurosci. / mRNA at the synapse: analysis of a synaptosomal preparation enriched in hippocampal dendritic spines by Chicurel M. E. (1993)
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{'key': 'e_1_2_2_27_1', 'first-page': '102a', 'article-title': 'Evidence that some of the proteins synthesized locally at synaptic sites are glycoproteins. (Abstr.)', 'volume': '115', 'author': 'Rao A.', 'year': '1991', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. / Evidence that some of the proteins synthesized locally at synaptic sites are glycoproteins. (Abstr.) by Rao A. (1991)
{'key': 'e_1_2_2_28_1', 'first-page': '379', 'article-title': 'Synaptosomal RNA: assessment of contamination by glia and comparison with total RNA', 'volume': '17', 'author': 'Rao A.', 'year': '1991', 'journal-title': 'Soc. Neurosci. Abstr.'} / Soc. Neurosci. Abstr. / Synaptosomal RNA: assessment of contamination by glia and comparison with total RNA by Rao A. (1991)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 6, 2006, 12:46 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 10:22 p.m.)
Indexed	2 months ago (June 26, 2025, 5:29 a.m.)
Issued	31 years, 11 months ago (Sept. 1, 1993)
Published	31 years, 11 months ago (Sept. 1, 1993)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	31 years, 11 months ago (Sept. 1, 1993)

Funders 0

None

BibTeX

@article{Rao_1993, title={Evaluation of RNAs Present in Synaptodendrosomes: Dendritic, Glial, and Neuronal Cell Body Contribution}, volume={61}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1993.tb03594.x}, DOI={10.1111/j.1471-4159.1993.tb03594.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Rao, Anuradha and Steward, Oswald}, year={1993}, month=sep, pages={835–844} }

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