DOI: 10.1111/j.1471-4159.1993.tb03216.x. Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by Brain‐Derived Neurotrophic Factor

Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by Brain‐Derived Neurotrophic Factor

10.1111/j.1471-4159.1993.tb03216.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: The neuropeptide‐inducing activity of neurotrophic factors was tested in cultured cerebral cortical neurons. Brain‐derived neurotrophic factor (BDNF) specifically increased contents of the neuropeptides somatostatin (SOM) and neuropeptide Y (NPY), but its effect on contents of cholecystokinin octapeptide and GABA was much less significant. The maximal induction of NPY content (15‐fold increase) was achieved by 20 ng/ml of BDNF. These changes were also reproduced at the mRNA level. In contrast, neurotrophin‐3 was much less potent at increasing NPY and SOM contents, and nerve growth factor had no effect on them. The expression of mRNA for NPY and SOM was fully dependent on the presence of BDNF in culture but irrelevant to the survival‐promoting activity of BDNF, which has been reported previously. Most of the NPY immunoreactivity induced by BDNF was colocalized with glutamate decarboxylase immunoreactivity in cultured cortical neurons. These results suggest that BDNF regulates the peptidergic expression of GABAergic neurons in the cerebral cortex.

Bibliography

Nawa, H., Bessho, Y., Carnahan, J., Nakanishi, S., & Mizuno, K. (1993). Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by BrainâDerived Neurotrophic Factor. Journal of Neurochemistry, 60(2), 772â775. Portico.

Authors 5

Hiroyuki Nawa (first)
Yasumasa Bessho (additional)
Josette Carnahan (additional)
Shigetada Nakanishi (additional)
Keiko Mizuno (additional)

References 26 Referenced 151

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{'key': 'e_1_2_2_25_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual by Sambrook J. (1989)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 6, 2006, 12:01 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 3:56 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 9:18 p.m.)
Issued	32 years, 7 months ago (Feb. 1, 1993)
Published	32 years, 7 months ago (Feb. 1, 1993)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	32 years, 7 months ago (Feb. 1, 1993)

Funders 0

None

BibTeX

@article{Nawa_1993, title={Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by Brain‐Derived Neurotrophic Factor}, volume={60}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1993.tb03216.x}, DOI={10.1111/j.1471-4159.1993.tb03216.x}, number={2}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Nawa, Hiroyuki and Bessho, Yasumasa and Carnahan, Josette and Nakanishi, Shigetada and Mizuno, Keiko}, year={1993}, month=feb, pages={772–775} }

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