DOI: 10.1111/j.1471-4159.1992.tb10090.x. Regulation of Neuronal Nitric Oxide and Cyclic GMP Formation by Ca<sup>2+</sup>

Regulation of Neuronal Nitric Oxide and Cyclic GMP Formation by Ca2+

10.1111/j.1471-4159.1992.tb10090.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract:Nitric oxide (NO) acts as a messenger molecule in the CNS by activating soluble guanylyl cyclase. Rat brain synaptosomal NO synthase was stimulated by Ca2+in a concentration‐dependent manner with half‐maximal effects observed at 0.3 μMand 0.2 μMwhen its activity was assayed as formation of NO and L‐citrulline, respectively. Cyclic GMP formation was apparently inhibited, however, at Ca2+concentrations required for the activation of NO synthase, indicating a down‐regulation of the signal in NO‐producing cells. Purified synaptosomal guanylyl cyclase was not inhibited directly by Ca2+, and the effect was not mediated by a protein binding to guanylyl cyclase at low or high Ca2+concentrations. In cytosolic fractions, the breakdown of cyclic GMP, but not that of cyclic AMP, was highly stimulated by Ca2+, and 3‐isobutyl‐1‐methylxanthine did not block this reaction effectively. The effects of Ca2+on cyclic GMP hydrolysis and on apparent guanylyl cyclase activities were abolished almost completely in the presence of the calmodulin antagonist calmidazolium, whose effect was attenuated by added calmodulin. Thus, a Ca2+/calmodulin‐dependent cyclic GMP phosphodiesterase is highly active in synaptic areas of the brain and may prevent elevations of intracellular cyclic GMP levels in activated, NO‐producing neurons.

Bibliography

Mayer, B., Klatt, P., BÃ¶hme, E., & Schmidt, K. (1992). Regulation of Neuronal Nitric Oxide and Cyclic GMP Formation by Ca2+. Journal of Neurochemistry, 59(6), 2024â2029. Portico.

Authors 4

Bernd Mayer (first)
Peter Klatt (additional)
Eycke Böhme (additional)
Kurt Schmidt (additional)

References 45 Referenced 147

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Dates

Type	When
Created	15 years, 1 month ago (July 28, 2010, 9:46 p.m.)
Deposited	6 months, 1 week ago (Feb. 23, 2025, 10:11 a.m.)
Indexed	2 months, 1 week ago (June 21, 2025, 2:03 a.m.)
Issued	32 years, 8 months ago (Dec. 1, 1992)
Published	32 years, 8 months ago (Dec. 1, 1992)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	32 years, 8 months ago (Dec. 1, 1992)

Funders 0

None

BibTeX

@article{Mayer_1992, title={Regulation of Neuronal Nitric Oxide and Cyclic GMP Formation by Ca2+}, volume={59}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1992.tb10090.x}, DOI={10.1111/j.1471-4159.1992.tb10090.x}, number={6}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Mayer, Bernd and Klatt, Peter and Böhme, Eycke and Schmidt, Kurt}, year={1992}, month=dec, pages={2024–2029} }

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