DOI: 10.1111/j.1471-4159.1992.tb08916.x. Inositol 1,4,5‐Trisphosphate Receptor Immunoreactivity in SH‐SY5Y Human Neuroblastoma Cells Is Reduced by Chronic Muscarinic Receptor Activation

Inositol 1,4,5‐Trisphosphate Receptor Immunoreactivity in SH‐SY5Y Human Neuroblastoma Cells Is Reduced by Chronic Muscarinic Receptor Activation

10.1111/j.1471-4159.1992.tb08916.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractInositol 1,4,5‐trisphosphate (InsP3) receptor immunoreactivity in SH‐SY5 Y human neuroblastoma cells was monitored with a monoclonal antibody raised against the mouse cerebellar InsP3 receptor. Recognition of a protein corresponding to the InsP3 receptor (molecular mass, ∼275 kDa) was inhibited markedly following exposure of cells to 0.1 mM carbachol. This effect was half‐maximal and maximal at ∼2 and ∼6 h, respectively; was blocked by atropine; but was not mimicked by thapsigargin, K+, or phorbol 12‐myristate 13‐acetate. However, the decrease in immunoreactivity following exposure of cells to carbachol for 5 h was blocked if the extracellular Ca2+ concentration was reduced from 1.3 mM to 200 nM. This manipulation also reduced markedly carbachol‐induced increases in InsP3 concentration at 5 h. These data indicate that chronic muscarinic stimulation of phosphoinositide hydrolysis reduces InsP3 receptor concentration in SH‐SY5 Y cells, perhaps via a mechanism that involves prolonged elevation of InsP3 levels.

Bibliography

Wojcikiewicz, R. J. H., Nakade, S., Mikoshiba, K., & Nahorski, S. R. (1992). Inositol 1,4,5âTrisphosphate Receptor Immunoreactivity in SHâSY5Y Human Neuroblastoma Cells Is Reduced by Chronic Muscarinic Receptor Activation. Journal of Neurochemistry, 59(1), 383â386. Portico.

Authors 4

Richard J. H. Wojcikiewicz (first)
Shinji Nakade (additional)
Katsuhiko Mikoshiba (additional)
Stefan R. Nahorski (additional)

References 22 Referenced 26

10.1038/341197a0
10.1042/bj2800205 / Biochem. J / Increased expression of the inositol 1,4,5‐trisphosphate receptor in human leukaemic (HL‐60) cells differentiated with retanoic acid or dimethyl sul‐phoxide. by Bradford P. G. (1991)
10.1042/bj2650421
10.1073/pnas.88.7.2951
10.1038/342032a0
10.1042/bj2550179
10.1042/bj2650555
10.1042/bj2730791 / Biochem. J / Accumulation and metabolism of Ins(1,4,5)P3 and Ins(l,3,4,5)P4 in muscarinic‐receptor‐stimulated SH‐SY5Y neuroblastoma cells. by Lambert D. G. (1990)
10.1016/0169-328X(90)90026-A
10.1073/pnas.87.23.9270
10.1016/S0021-9258(18)81867-1 / J. Biol. Chem / Hormonal regulation of inositol 1,4,5‐trisphosphate receptor in rat liver. by Mauger J.‐P. (1989)
10.1016/S0021-9258(19)38397-8 / Biol. Chem / Structure and expression of the rat inositol 1,4,5‐trisphosphate receptor. J. by Mignery G. A. (1990)
10.1073/pnas.88.11.4911
10.1042/bj2770125
10.1073/pnas.88.14.6244
10.1016/0014-5793(91)80465-F
10.1016/0014-5793(88)81258-4
10.1016/S0021-9258(18)63852-9 / J. Biol Chem / Activation of Ca2+ entry by the tumor promoter thapsi‐gargin in parotid acinar cells. by Takemura H. (1989)
10.1073/pnas.87.7.2466
10.1042/bj2690211
10.1016/S0021-9258(18)81723-9 / Biol. Chem / Inhibition of inositol 1,4,5‐tris‐phosphate‐induced Ca2+ release in permeabilized pancreatic acinar cells by hormonal and phorbol ester pretreatment. J. by Willems P. H. G. M. (1989)
10.1016/S0021-9258(18)54559-2 / J. Biol. Chem / Chronic muscarinic stimulation of SH‐SY5Y neuroblastoma cells suppresses inositol 1,4,5‐trisphosphate action. by Wojcikiewicz R. J. H. (1991)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 11:06 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 2:57 p.m.)
Indexed	1 year, 7 months ago (Feb. 5, 2024, 8:07 a.m.)
Issued	33 years, 2 months ago (July 1, 1992)
Published	33 years, 2 months ago (July 1, 1992)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	33 years, 2 months ago (July 1, 1992)

Funders 0

None

BibTeX

@article{Wojcikiewicz_1992, title={Inositol 1,4,5‐Trisphosphate Receptor Immunoreactivity in SH‐SY5Y Human Neuroblastoma Cells Is Reduced by Chronic Muscarinic Receptor Activation}, volume={59}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1992.tb08916.x}, DOI={10.1111/j.1471-4159.1992.tb08916.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Wojcikiewicz, Richard J. H. and Nakade, Shinji and Mikoshiba, Katsuhiko and Nahorski, Stefan R.}, year={1992}, month=jul, pages={383–386} }

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