DOI: 10.1111/j.1471-4159.1992.tb08458.x. Brain Protein Synthesis in the Conscious Rat Using L‐[<sup>35</sup>S]Methionine: Relationship of Methionine Specific Activity Between Plasma and Precursor Compartment and Evaluation of Methionine Metabolic Pathways

Brain Protein Synthesis in the Conscious Rat Using L‐[35S]Methionine: Relationship of Methionine Specific Activity Between Plasma and Precursor Compartment and Evaluation of Methionine Metabolic Pathways

10.1111/j.1471-4159.1992.tb08458.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: The method previously developed for the measurement of rates of methionine incorporation into brain proteins assumed that methionine derived from protein degradation did not recycle into the precursor pool for protein synthesis and that the metabolism of methionine via the transmethylation pathway was negligible. To evaluate the degree of recycling, we have compared, under steady‐state conditions, the specific activity of L‐[35S]methionine in the tRNA‐bound pool to that of plasma. The relative contribution of methionine from protein degradation to the precursor pool was 26%. Under the same conditions, the relative rate of methionine flux into the transmethylation cycle was estimated to be 10% of the rate of methionine incorporation into brain proteins. These results indicate the following: (a) there is significant recycling of unlabeled methionine derived from protein degradation in brain; and (b) the metabolism of methionine is directed mainly towards protein synthesis. At normal plasma amino acid levels, methionine is the amino acid which, to date, presents the lowest degree of dilution in the precursor pool for protein synthesis. L‐[35S]‐Methionine, therefore, presents radiobiochemical properties required to measure, with minimal underestimation, rates of brain protein synthesis in vivo.

Bibliography

Grange, E., Gharib, A., Lepetit, P., Guillaud, J., Sarda, N., & Bobillier, P. (1992). Brain Protein Synthesis in the Conscious Rat Using Lâ[35S]Methionine: Relationship of Methionine Specific Activity Between Plasma and Precursor Compartment and Evaluation of Methionine Metabolic Pathways. Journal of Neurochemistry, 59(4), 1437â1443. Portico.

Authors 6

Eric Grange (first)
Abdallah Gharib (additional)
Patrick Lepetit (additional)
Josette Guillaud (additional)
Nicole Sarda (additional)
Pierre Bobillier (additional)

References 37 Referenced 22

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{'issue': '1', 'key': 'e_1_2_2_3_1', 'first-page': 'S17', 'article-title': 'Regional human brain uptake and protein incorporation of L‐(11C)methionine studied in vivo by PET', 'volume': '1', 'author': 'Bustany P.', 'year': '1981', 'journal-title': 'J. Cereb. Blood Flow Metab'} / J. Cereb. Blood Flow Metab / Regional human brain uptake and protein incorporation of L‐(11C)methionine studied in vivo by PET by Bustany P. (1981)
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{'key': 'e_1_2_2_13_1', 'first-page': '255', 'article-title': 'Biosynthèse des protéines cérébrates chez le rat vigile: équilibre de la L‐méthionine entre le plasma et le com‐partiment précurseur direct.', 'volume': '312', 'author': 'Grange E.', 'year': '1991', 'journal-title': 'C. R. Acad. Sci. [III]'} / C. R. Acad. Sci. [III] / Biosynthèse des protéines cérébrates chez le rat vigile: équilibre de la L‐méthionine entre le plasma et le com‐partiment précurseur direct. by Grange E. (1991)
{'issue': '2', 'key': 'e_1_2_2_14_1', 'first-page': 'S356', 'article-title': 'Brain protein synthesis in conscious rats: equilibration of L‐[35S]methionine specific activity between plasma and methio‐nyl‐tRNA', 'volume': '11', 'author': 'Grange E.', 'year': '1991', 'journal-title': 'J. Cereb. Blood Flow Metab'} / J. Cereb. Blood Flow Metab / Brain protein synthesis in conscious rats: equilibration of L‐[35S]methionine specific activity between plasma and methio‐nyl‐tRNA by Grange E. (1991)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 11:25 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 12:37 p.m.)
Indexed	1 year, 10 months ago (Oct. 25, 2023, 6:04 p.m.)
Issued	32 years, 11 months ago (Oct. 1, 1992)
Published	32 years, 11 months ago (Oct. 1, 1992)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	32 years, 11 months ago (Oct. 1, 1992)

Funders 0

None

BibTeX

@article{Grange_1992, title={Brain Protein Synthesis in the Conscious Rat Using L‐[35S]Methionine: Relationship of Methionine Specific Activity Between Plasma and Precursor Compartment and Evaluation of Methionine Metabolic Pathways}, volume={59}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1992.tb08458.x}, DOI={10.1111/j.1471-4159.1992.tb08458.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Grange, Eric and Gharib, Abdallah and Lepetit, Patrick and Guillaud, Josette and Sarda, Nicole and Bobillier, Pierre}, year={1992}, month=oct, pages={1437–1443} }

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