DOI: 10.1111/j.1471-4159.1991.tb08250.x. Recombinant Human and Rat Ciliary Neurotrophic Factors

Recombinant Human and Rat Ciliary Neurotrophic Factors

10.1111/j.1471-4159.1991.tb08250.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractThe human ciliary neurotrophic factor (CNTF) gene was identified and cloned, based on homology with the recently cloned rat cDNA. The gene encodes a protein of 200 amino acids, which shares about 80% sequence identity with rat and rabbit CNTF and, like these homologues, lacks an apparent secretion signal sequence. The human CNTF gene, like the rat gene, appears to contain a single intron separating two protein coding exons. An intronless human CNTF gene was constructed by the use of polymerase chain reactions and introduced into vectors designed for expression of foreign proteins in E. coli. The rat CNTF gene was also introduced into similar vectors. Both the human and rat proteins were expressed at exceptionally high levels, at 20–40% and 60–70% of total protein, respectively. Extraction of the recombinant proteins from inclusion bodies by guanidinium chloride, followed by two column chromatography steps, produced high yields of pure CNTF that supported survival and neurite outgrowth from embryonic chick ciliary neurons in culture. The biological activity of both recombinant proteins was comparable to that of native rat CNTF.

Bibliography

Masiakowski, P., Liu, H., Radziejewski, C., Lottspeich, F., Oberthuer, W., Wong, V., Lindsay, R. M., Furth, M. E., & Panayotatos, N. (1991). Recombinant Human and Rat Ciliary Neurotrophic Factors. Journal of Neurochemistry, 57(3), 1003â1012. Portico.

Authors 9

Piotr Masiakowski (first)
Haoxing Liu (additional)
Czeslaw Radziejewski (additional)
Friedrich Lottspeich (additional)
Walter Oberthuer (additional)
Vivien Wong (additional)
Ronald M. Lindsay (additional)
Mark E. Furth (additional)
Nikos Panayotatos (additional)

References 28 Referenced 79

10.1016/0006-8993(80)90043-8
10.1126/science.451576
{'key': 'e_1_2_2_4_1', 'volume-title': 'Current Protocols in Molecular Biology', 'author': 'Ausubel F. M.', 'year': '1989'} / Current Protocols in Molecular Biology by Ausubel F. M. (1989)
10.1111/j.1471-4159.1984.tb05410.x
10.1073/pnas.75.10.4853
{'key': 'e_1_2_2_7_1', 'volume-title': 'From Gene to Protein: Steps Dictating the Maximal Levels of Gene Expression', 'author': 'Buell G.', 'year': '1986'} / From Gene to Protein: Steps Dictating the Maximal Levels of Gene Expression by Buell G. (1986)
10.1016/0896-6273(90)90120-5
10.1016/0896-6273(89)90312-7
10.1146/annurev.ne.03.030180.002033
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{'key': 'e_1_2_2_19_1', 'first-page': '77', 'volume-title': 'Growth and Maturation Factors', 'author': 'Manthorpe M.', 'year': '1985'} / Growth and Maturation Factors by Manthorpe M. (1985)
{'key': 'e_1_2_2_20_1', 'first-page': '163', 'volume-title': 'Plasmids: A Practical Approach', 'author': 'Panayotatos N.', 'year': '1987'} / Plasmids: A Practical Approach by Panayotatos N. (1987)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:02 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 3:53 a.m.)
Indexed	2 months, 2 weeks ago (June 11, 2025, 5:29 a.m.)
Issued	33 years, 11 months ago (Sept. 1, 1991)
Published	33 years, 11 months ago (Sept. 1, 1991)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	33 years, 11 months ago (Sept. 1, 1991)

Funders 0

None

BibTeX

@article{Masiakowski_1991, title={Recombinant Human and Rat Ciliary Neurotrophic Factors}, volume={57}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1991.tb08250.x}, DOI={10.1111/j.1471-4159.1991.tb08250.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Masiakowski, Piotr and Liu, Haoxing and Radziejewski, Czeslaw and Lottspeich, Friedrich and Oberthuer, Walter and Wong, Vivien and Lindsay, Ronald M. and Furth, Mark E. and Panayotatos, Nikos}, year={1991}, month=sep, pages={1003–1012} }

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