DOI: 10.1111/j.1471-4159.1991.tb06404.x. Solubilization, Characterization, and Partial Purification of α‐Amino‐3‐Hydroxy‐5‐Methyl‐4‐Isoxazolepropionic Acid‐, Quisqualate‐, Kainate‐Sensitive <scp>l</scp>‐Glutamate Binding Sites from Porcine Brain Synaptic Junctions

Solubilization, Characterization, and Partial Purification of α‐Amino‐3‐Hydroxy‐5‐Methyl‐4‐Isoxazolepropionic Acid‐, Quisqualate‐, Kainate‐Sensitive l‐Glutamate Binding Sites from Porcine Brain Synaptic Junctions

10.1111/j.1471-4159.1991.tb06404.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract l‐[3H]Glutamate binding sites were solubilized from porcine brain synaptic junctions by Triton X‐114 in the presence of KC1. The solubilized binding sites bound l‐[3H]glutamate reversibly with Kd and Bmax values of 1.48 ± 0.18 μM and 178.2 ± 15.9 pmol/mg of protein, respectively. These binding sites appeared to be integral membrane glycoproteins, with sugar moieties recognized by wheat germ agglutinin. A 49.3‐fold purification of these binding sites was achieved by Triton X‐114 solubilization, anion‐exchange chromatography, and affinity chromatography using wheat germ agglutinin‐Sepharose. The apparent molecular mass of the partially purified binding sites was 620 ± 50 kDa. l‐[3H]Glutamate bound to the solubilized preparation could be effectively displaced by agonists of non‐N‐methyl‐d‐as‐partate (NMDA) l‐glutamate receptors but not by NMDA or α‐amino‐4‐phosphonobutyrate. The rank order for the competitive ligands in displacing l‐[3H]glutamate was: quisqualate > α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid > l‐glutamate > kainate.

Bibliography

Chang, Y., Lin, Y., Lee, Y., & Leng, C. (1991). Solubilization, Characterization, and Partial Purification of Î±âAminoâ3âHydroxyâ5âMethylâ4âIsoxazolepropionic Acidâ, Quisqualateâ, KainateâSensitive lâGlutamate Binding Sites from Porcine Brain Synaptic Junctions. Journal of Neurochemistry, 57(6), 1921â1926. Portico.

Authors 4

Yen‐Chung Chang (first)
Yih‐Hong Lin (additional)
Yun‐Huey Lee (additional)
Chih‐Hsiang Leng (additional)

References 29 Referenced 9

10.1016/S0021-9258(19)69848-0
10.1126/science.2168579
10.1016/0197-0186(90)90085-8
10.1016/S0021-9258(19)57409-9 / J. Biol. Chem. / Immune labeling and purification of a 71 kDa glutamate‐binding protein from brain synaptic membranes by Chen J.‐W. (1988)
10.1146/annurev.ne.13.030190.001021
{'key': 'e_1_2_2_7_1', 'first-page': '668', 'article-title': 'Insulin receptors', 'volume': '34', 'author': 'Cuatrecasas P.', 'year': '1974', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. / Insulin receptors by Cuatrecasas P. (1974)
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10.1016/0896-6273(90)90033-C

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:17 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 3:59 a.m.)
Indexed	1 year, 1 month ago (July 17, 2024, 5:52 p.m.)
Issued	33 years, 9 months ago (Dec. 1, 1991)
Published	33 years, 9 months ago (Dec. 1, 1991)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	33 years, 9 months ago (Dec. 1, 1991)

Funders 0

None

BibTeX

@article{Chang_1991, title={Solubilization, Characterization, and Partial Purification of α‐Amino‐3‐Hydroxy‐5‐Methyl‐4‐Isoxazolepropionic Acid‐, Quisqualate‐, Kainate‐Sensitive <scp>l</scp>‐Glutamate Binding Sites from Porcine Brain Synaptic Junctions}, volume={57}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1991.tb06404.x}, DOI={10.1111/j.1471-4159.1991.tb06404.x}, number={6}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Chang, Yen‐Chung and Lin, Yih‐Hong and Lee, Yun‐Huey and Leng, Chih‐Hsiang}, year={1991}, month=dec, pages={1921–1926} }

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