DOI: 10.1111/j.1471-4159.1991.tb02597.x. Recovery of Postischemic Brain Metabolism and Function Following Treatment with a Free Radical Scavenger and Platelet‐Activating Factor Antagonists

Recovery of Postischemic Brain Metabolism and Function Following Treatment with a Free Radical Scavenger and Platelet‐Activating Factor Antagonists

10.1111/j.1471-4159.1991.tb02597.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: We have studied the metabolic and functional effects of two new platelet‐activating factor (PAF) antagonists (BN 50726 and BN 50739) and their diluent (dimethyl sulfoxide; DMSO) during reoxygenation of the 14‐min ischemic isolated brain. Blood gases, EEG, auditory evoked potentials, cerebral metabolic rate for glucose (CMRglc), and cerebral metabolic rate for oxygen (CMRO2) were monitored throughout the study. Frozen brain samples were taken for measurement of brain tissue high‐energy phosphates, carbohydrate content, and thiobarbituric acid‐reactive material (TBAR, an indicator of lipid peroxidation) at the end of the study. Following 60 min of reoxygenation in the nontreated 14‐min ischemic brains, lactate, AMP, creatine (Cr), intracellular hydrogen ion concentration [H+]i), and TBAR values were significantly higher and ATP, creatine phosphate (PCr), CMRglc, CMRO2, and energy charge (EC) values were significantly lower than the corresponding normoxic control values. PCr and CMRO2 values were significantly higher, and glycogen, AMP, and [H+]i values were significantly lower in the BN 50726‐treated ischemic brains than in DMSO‐treated ischemic brains. In brains treated with BN 50739, ATP, ADP, PCr, CMRO2, and EC values were significantly higher, and lactate, AMP, Cr, and [H+]i values were significantly lower than corresponding values in the DMSO‐treated ischemic brains. TBAR values were near control levels in all brains exposed to DMSO. There was also marked recovery of EEG and auditory evoked potentials in brains treated with DMSO. Treatment with BN 50726 or BN 50739 in DMSO appeared to improve brain mitochondrial function and energy metabolism partly as the result of DMSO action as a free radical scavenger. The PAF antagonists act at a different level, possibly restoring normal mitochondrial function or preventing some of the adverse effects of increased excitatory amino acid and/or intracellular Ca2+ levels. This is the only drug combination currently known to promote such complete short‐term metabolic and functional recovery during postischemic reoxygenation.

Bibliography

Gilboe, D. D., Kintner, D., Fitzpatric, J. H., Emoto, S. E., Esanu, A., Braquet, P. G., & Bazan, N. G. (1991). Recovery of Postischemic Brain Metabolism and Function Following Treatment with a Free Radical Scavenger and PlateletâActivating Factor Antagonists. Journal of Neurochemistry, 56(1), 311â319. Portico.

Authors 7

D. D. Gilboe (first)
D. Kintner (additional)
J. H. Fitzpatric (additional)
S. E. Emoto (additional)
A. Esanu (additional)
P. G. Braquet (additional)
N. G. Bazan (additional)

References 33 Referenced 72

10.1016/0005-2760(70)90086-X
10.1007/978-1-4684-0955-0_26
{'key': 'e_1_2_2_4_1', 'first-page': '197', 'article-title': 'Membrane lipids in the pathogenesis of brain edema: phospholipids and arachidonic acid, the earliest membrane components changed at the onset of ischemia', 'volume': '28', 'author': 'Bazan N. G.', 'year': '1980', 'journal-title': 'Adv. Neurol.'} / Adv. Neurol. / Membrane lipids in the pathogenesis of brain edema: phospholipids and arachidonic acid, the earliest membrane components changed at the onset of ischemia by Bazan N. G. (1980)
10.1111/j.1471-4159.1988.tb01175.x
BraquetP.andEsanuA.(1989)Patent pending.
10.2491/jjsth1970.16.558
{'key': 'e_1_2_2_8_1', 'first-page': '97', 'article-title': 'Perspectives in platelet‐activating factor research', 'volume': '39', 'author': 'Braquet P.', 'year': '1987', 'journal-title': 'Pharmacol. Rev.'} / Pharmacol. Rev. / Perspectives in platelet‐activating factor research by Braquet P. (1987)
{'key': 'e_1_2_2_9_1', 'first-page': '75', 'article-title': 'PAF/cytokine auto‐generated feedback networks in microvascular immune injury: consequences in shock, ischemia, and graft rejection', 'volume': '1', 'author': 'Braquet P.', 'year': '1989', 'journal-title': 'J. Lipid Mediators'} / J. Lipid Mediators / PAF/cytokine auto‐generated feedback networks in microvascular immune injury: consequences in shock, ischemia, and graft rejection by Braquet P. (1989)
{'key': 'e_1_2_2_10_1', 'first-page': '43', 'article-title': 'Free radicals as mediators of tissue injury', 'volume': '492', 'author': 'Maestro R. F.', 'year': '1980', 'journal-title': 'Acta Physiol. Scand.'} / Acta Physiol. Scand. / Free radicals as mediators of tissue injury by Maestro R. F. (1980)
{'key': 'e_1_2_2_11_1', 'first-page': '491', 'volume-title': 'Oxygen and Physiological Function', 'author': 'Demopoulos H.', 'year': '1977'} / Oxygen and Physiological Function by Demopoulos H. (1977)
10.1111/j.1471-4159.1988.tb02502.x
10.1016/0003-9861(86)90557-6
10.1152/jappl.1973.34.4.534 / J. Appl. Physiol. / A guide for the isolation of the canine brain by Gilboe D. D. (1973)
{'key': 'e_1_2_2_15_1', 'first-page': '169', 'volume-title': 'Oxygen Transport to Tissue—VI', 'author': 'Gilboe D. D.', 'year': '1985'} / Oxygen Transport to Tissue—VI by Gilboe D. D. (1985)
{'key': 'e_1_2_2_16_1', 'first-page': '119', 'volume-title': 'Pharmacology of Cerebral Ischemia', 'author': 'Gilboe D. D.', 'year': '1986'} / Pharmacology of Cerebral Ischemia by Gilboe D. D. (1986)
10.1002/jnr.490200407
10.1016/0011-2240(86)90014-3
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10.1016/0006-291X(87)90407-4
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10.1038/jcbfm.1989.9
10.1016/0011-2240(82)90125-0
10.1038/jcbfm.1981.18
{'key': 'e_1_2_2_31_1', 'first-page': '141', 'volume-title': 'Statistical Methods', 'author': 'Snedecor G. W.', 'year': '1980'} / Statistical Methods by Snedecor G. W. (1980)
{'key': 'e_1_2_2_32_1', 'first-page': '1', 'volume-title': 'Dimethyl Sulfoxide. Vol. 1, Basic Concepts of DMSO', 'author': 'Szmant H. H.', 'year': '1971'} / Dimethyl Sulfoxide. Vol. 1, Basic Concepts of DMSO by Szmant H. H. (1971)
10.3171/jns.1980.53.3.0323
10.1016/0192-0561(87)90050-6

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 9:05 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:21 p.m.)
Indexed	1 month, 4 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	34 years, 8 months ago (Jan. 1, 1991)
Published	34 years, 8 months ago (Jan. 1, 1991)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	34 years, 8 months ago (Jan. 1, 1991)

Funders 0

None

BibTeX

@article{Gilboe_1991, title={Recovery of Postischemic Brain Metabolism and Function Following Treatment with a Free Radical Scavenger and Platelet‐Activating Factor Antagonists}, volume={56}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1991.tb02597.x}, DOI={10.1111/j.1471-4159.1991.tb02597.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Gilboe, D. D. and Kintner, D. and Fitzpatric, J. H. and Emoto, S. E. and Esanu, A. and Braquet, P. G. and Bazan, N. G.}, year={1991}, month=jan, pages={311–319} }

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