DOI: 10.1111/j.1471-4159.1990.tb04879.x. Poly‐ and Monoclonal Antibodies Against Recombinant Rat Brain Calbindin D‐28K Were Produced to Map Its Selective Distribution in the Central Nervous System

Poly‐ and Monoclonal Antibodies Against Recombinant Rat Brain Calbindin D‐28K Were Produced to Map Its Selective Distribution in the Central Nervous System

10.1111/j.1471-4159.1990.tb04879.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Many processes in the CNS depend on calcium. The calcium signal is transduced into an intracellular response via Ca2+‐binding proteins, including calbindin D‐28K. In many laboratories, polyclonal antibodies against chicken in testinal calbindin D‐28K have been used to study its localization in the brain (normal and degenerated) of various species, including humans, but some of these antisera cross‐reacted with other proteins, including calretinin. We purified recombinant rat brain calbindin D‐28K to raise antisera in rabbits and purified a recombinant rat–chicken calbindin D28K hybrid protein to immunize mice for the generation of monoclonal antibodies. These antisera were highly specific for calbindin D‐28K, as demonstrated by two‐dimensional Western blotting analysis. Immunohistochemical analyses combined with in situ hybridization studies demonstrated that calbindin D‐28K in the Purkinje cells of the cerebellum is independent of vitamin D. The antibodies described here will be important tools for studying the regulation of expression of calbindin D‐28K and its biological function in the brain and in the PNS.

Bibliography

Pinol, M. R., KÃ¤gi, U., Heizmann, C. W., Vogel, B., SÃ©quier, J., Haas, W., & Hunziker, W. (1990). Polyâ and Monoclonal Antibodies Against Recombinant Rat Brain Calbindin Dâ28K Were Produced to Map Its Selective Distribution in the Central Nervous System. Journal of Neurochemistry, 54(6), 1827â1833. Portico.

Authors 7

M. Rosa Pinol (first)
Urs Kägi (additional)
Claus W. Heizmann (additional)
Brigitte Vogel (additional)
Jean‐Marc Séquier (additional)
Werner Haas (additional)
Willi Hunziker (additional)

References 39 Referenced 75

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{'key': 'e_1_2_2_16_1', 'volume-title': 'The Role of Calcium in Biological Systems. Vol. 5', 'author': 'Heizmann C. W.', 'year': '1989'} / The Role of Calcium in Biological Systems. Vol. 5 by Heizmann C. W. (1989)
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{'key': 'e_1_2_2_22_1', 'first-page': '233', 'volume-title': 'Vitamin D, Chemical, Biochemical, and Clinical Endocrinology', 'author': 'Kretsinger R. H.', 'year': '1982'} / Vitamin D, Chemical, Biochemical, and Clinical Endocrinology by Kretsinger R. H. (1982)
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{'key': 'e_1_2_2_30_1', 'first-page': '24', 'volume-title': 'Basic and Clinical Immunology', 'author': 'Sternberger L. A.', 'year': '1979'} / Basic and Clinical Immunology by Sternberger L. A. (1979)
10.1002/cne.902620409
10.1126/science.2847315
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{'key': 'e_1_2_2_34_1', 'first-page': '109', 'volume-title': 'Cellular Calcium and Phosphate Transport in Health and Disease', 'author': 'Taylor A. N.', 'year': '1988'} / Cellular Calcium and Phosphate Transport in Health and Disease by Taylor A. N. (1988)
{'key': 'e_1_2_2_35_1', 'first-page': 'E483', 'article-title': 'Rat calcium binding proteins: distribution, development and vitamin D dependence', 'volume': '243', 'author': 'Thomasset M.', 'year': '1982', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. / Rat calcium binding proteins: distribution, development and vitamin D dependence by Thomasset M. (1982)
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10.1177/37.7.2732461
10.1016/S0021-9258(19)81585-5 / J. Biol. Chem. / Analysis of rat vitamin D‐dependent calbindin D‐28K gene expression by Varghese S. (1988)
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{'key': 'e_1_2_2_40_1', 'first-page': '147', 'volume-title': 'Das Tier im Experiment', 'author': 'Weiser H. W.', 'year': '1978'} / Das Tier im Experiment by Weiser H. W. (1978)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 10:20 a.m.)
Indexed	1 year, 7 months ago (Jan. 31, 2024, 10:49 a.m.)
Issued	35 years, 3 months ago (June 1, 1990)
Published	35 years, 3 months ago (June 1, 1990)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	35 years, 3 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{Pinol_1990, title={Poly‐ and Monoclonal Antibodies Against Recombinant Rat Brain Calbindin D‐28K Were Produced to Map Its Selective Distribution in the Central Nervous System}, volume={54}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1990.tb04879.x}, DOI={10.1111/j.1471-4159.1990.tb04879.x}, number={6}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Pinol, M. Rosa and Kägi, Urs and Heizmann, Claus W. and Vogel, Brigitte and Séquier, Jean‐Marc and Haas, Werner and Hunziker, Willi}, year={1990}, month=jun, pages={1827–1833} }

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