DOI: 10.1111/j.1471-4159.1990.tb04187.x. Dephosphorylation of r Factor by Protein Phosphatase 2A in Synaptosomal Cytosol Fractions, and Inhibition by Aluminum

Dephosphorylation of r Factor by Protein Phosphatase 2A in Synaptosomal Cytosol Fractions, and Inhibition by Aluminum

10.1111/j.1471-4159.1990.tb04187.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractWhen the synaptosomal cytosol fraction from rat brain was chromatographed on a DEAE‐cellulose column and assayed for protein phosphatases for τ factor and histone H1, two peaks of activities, termed peak 1 (major) and peak 2 (minor), were separated. Each peak was in a single form on Sephacryl S‐300 column chromatography. Both peaks 1 and 2 dephosphorylated τ factor phosphorylated by Ca2+/calmodulin‐dependent protein kinase II and the catalytic subunit of cyclic AMP‐dependent protein kinase. The Km values were in the range of 0.42–0.84 μM for τ factor. There were no differences in kinetic properties of dephosphorylation between the substrates phosphorylated by the two kinases. The phosphatase activities did not depend on Ca2+, Mn2+, and Mg2+. Immunoprecipitation and immunoblotting analysis using polyclonal antibodies to the catalytic subunit of brain protein phosphatase 2A revealed that both protein phosphatases are the holoenzymic forms of protein phosphatase 2A. Aluminum chloride inhibited the activities of both peaks 1 and 2 with IC50 values of 40–60 μM. These results suggest that dephosphorylation of r factor in presynaptic nerve terminals is controlled mainly by protein phosphatase 2A and that the neurotoxic effect of aluminum seems to be related mostly to inhibition of dephosphorylation of τ factor

Bibliography

Yamamoto, H., Saitoh, Y., Yasugawa, S., & Miyamoto, E. (1990). Dephosphorylation of r Factor by Protein Phosphatase 2A in Synaptosomal Cytosol Fractions, and Inhibition by Aluminum. Journal of Neurochemistry, 55(2), 683â690. Portico.

Authors 4

Hideyuki Yamamoto (first)
Yoshiki Saitoh (additional)
Setsuko Yasugawa (additional)
Eishichi Miyamoto (additional)

References 55 Referenced 50

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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 8:24 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 2:16 p.m.)
Indexed	1 week ago (Aug. 30, 2025, 12:40 p.m.)
Issued	35 years, 1 month ago (Aug. 1, 1990)
Published	35 years, 1 month ago (Aug. 1, 1990)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	35 years, 1 month ago (Aug. 1, 1990)

Funders 0

None

BibTeX

@article{Yamamoto_1990, title={Dephosphorylation of r Factor by Protein Phosphatase 2A in Synaptosomal Cytosol Fractions, and Inhibition by Aluminum}, volume={55}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1990.tb04187.x}, DOI={10.1111/j.1471-4159.1990.tb04187.x}, number={2}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Yamamoto, Hideyuki and Saitoh, Yoshiki and Yasugawa, Setsuko and Miyamoto, Eishichi}, year={1990}, month=aug, pages={683–690} }

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