DOI: 10.1111/j.1471-4159.1990.tb01217.x. Depolarization‐Induced Phosphorylation of the Protein Kinase C Substrate B‐50 (GAP‐43) in Rat Cortical Synaptosomes

Depolarization‐Induced Phosphorylation of the Protein Kinase C Substrate B‐50 (GAP‐43) in Rat Cortical Synaptosomes

10.1111/j.1471-4159.1990.tb01217.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: We studied the molecular events underlying K+‐induced phosphorylation of the neuron‐specific protein kinase C substrate B‐50. Rat cortical synaptosomes were prelabelled with 32P‐labelled orthophosphate. B‐50 phosphorylation was measured by an immunoprecipitation assay. In this system, various phorbol esters, as well as a synthetic diacylglycerol derivative, enhance B‐50 phosphorylation. K+ depolarization induces a transient enhancement of B‐50 phosphorylation, which is totally dependent on extracellular Ca2+. Also, the application of the Ca2+ ionophore A23187 induces B‐50 phosphorylation, but the magnitude and kinetics of A23187‐induced B‐50 phosphorylation differ from those induced by depolarization. The protein kinase inhibitors 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine (H‐7), N‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalenesulfonamide (W‐7), and staurosporine antagonize K+‐ as well as PDB‐induced B‐50 phosphorylation, whereas trifluoperazine and calmidazolium are ineffective under both conditions. We suggest that elevation of the intracellular Ca2+ level after depolarization is a trigger for activation of protein kinase C, which subsequently phosphorylates its substrate B‐50. This sequence of events could be of importance for the mechanism of depolarization‐induced transmitter release.

Bibliography

Dekker, L. V., De Graan, P. N. E., De Wit, M., Hens, J. J. H., & Gispen, W. H. (1990). DepolarizationâInduced Phosphorylation of the Protein Kinase C Substrate Bâ50 (GAPâ43) in Rat Cortical Synaptosomes. Journal of Neurochemistry, 54(5), 1645â1652. Portico.

Authors 5

L. V. Dekker (first)
P. N. E. De Graan (additional)
M. De Wit (additional)
J. J. H. Hens (additional)
W. H. Gispen (additional)

References 49 Referenced 56

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10.1113/jphysiol.1976.sp011241
{'key': 'e_1_2_2_11_1', 'first-page': '232', 'article-title': 'Staurosporine counteracts the phorbol ester‐induced enhancement of neurotransmitter release in hippocampus', 'volume': '296', 'author': 'Daschmann B.', 'year': '1988', 'journal-title': 'Arch. Int. Pharmacodyn. Ther.'} / Arch. Int. Pharmacodyn. Ther. / Staurosporine counteracts the phorbol ester‐induced enhancement of neurotransmitter release in hippocampus by Daschmann B. (1988)
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{'key': 'e_1_2_2_13_1', 'first-page': '175', 'article-title': 'Phorbol esters induce long‐ and short‐term enhancement of B‐50/GAP‐43 phosphorylation in hippocampal slices', 'volume': '3', 'author': 'Graan P. N. E.', 'year': '1988', 'journal-title': 'Neurosci. Res. Commun.'} / Neurosci. Res. Commun. / Phorbol esters induce long‐ and short‐term enhancement of B‐50/GAP‐43 phosphorylation in hippocampal slices by Graan P. N. E. (1988)
10.1111/j.1471-4159.1989.tb10892.x
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{'key': 'e_1_2_2_37_1', 'first-page': '197.15', 'article-title': 'Functional significance and localization of phosphosite(s) in the neuronspecific protein B‐50/GAP‐43', 'volume': '14', 'author': 'Schrama L. H.', 'year': '1988', 'journal-title': 'Soc. Neurosci. Abstr.'} / Soc. Neurosci. Abstr. / Functional significance and localization of phosphosite(s) in the neuronspecific protein B‐50/GAP‐43 by Schrama L. H. (1988)
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{'key': 'e_1_2_2_49_1', 'first-page': '243', 'volume-title': 'Neuromodulation', 'author': 'Zucker R. S.', 'year': '1987'} / Neuromodulation by Zucker R. S. (1987)
10.1111/j.1471-4159.1980.tb11262.x

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 8:33 p.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 4:10 a.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 7:27 p.m.)
Issued	35 years, 4 months ago (May 1, 1990)
Published	35 years, 4 months ago (May 1, 1990)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	35 years, 4 months ago (May 1, 1990)

Funders 0

None

BibTeX

@article{Dekker_1990, title={Depolarization‐Induced Phosphorylation of the Protein Kinase C Substrate B‐50 (GAP‐43) in Rat Cortical Synaptosomes}, volume={54}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1990.tb01217.x}, DOI={10.1111/j.1471-4159.1990.tb01217.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Dekker, L. V. and De Graan, P. N. E. and De Wit, M. and Hens, J. J. H. and Gispen, W. H.}, year={1990}, month=may, pages={1645–1652} }

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