DOI: 10.1111/j.1471-4159.1989.tb11765.x. Synthesis of <scp>l</scp>‐3,4‐Bihydroxyphenylalanine by Tyrosine Hydroxylase cDNA‐Transfected C6 Cells: Application for Intracerebral Grafting

Synthesis of l‐3,4‐Bihydroxyphenylalanine by Tyrosine Hydroxylase cDNA‐Transfected C6 Cells: Application for Intracerebral Grafting

10.1111/j.1471-4159.1989.tb11765.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractIn the present study, we obtained genetically manipulated nonneuronal cells which synthesize a catecholamine precursor for future use in intracerebral grafting. Human type 1 tyrosine hydroxylase (TH; EC 1.14.16.2) cDNA was inserted into eukaryotic expression vector pKCRH2 and was co‐transfected into C6 cells with plasmid pSV2neo. Expression of the TH minigene was screened by immuno‐histochemical staining with TH antibody and immunoblot‐ting analysis. Several clones of the C6 transfectahts that produce TH molecules were obtained. These cells showed TH activity, and the product, L‐3,4‐dihydroxyphenylalanine (L‐DOPA), was detected intracellulary due to the ajbsence of L‐amino acid decarboxylase (EC 4.1.1.28) activity. It was found that a large amount of L‐DOPA was released from the cells into the culture medium. These transfectants were transplanted into rat brain, and the expression of TH was examined immunohistochemically. On the 10th day following transplantation, a mass of C6 cells which was heavily stained with TH antibody was observed in the brain. These findings may provide us with an opportunity to investigate the effects of intracerebral transplantation of nonneuronal cells that produce catecholamine or its precursor.

Bibliography

Uchida, K., Takamatsu, K., Kaneda, N., Toya, S., Tsukada, Y., Kurosawa, Y., Fujita, K., Nagatsu, T., & Kohsaka, S. (1989). Synthesis of lâ3,4âBihydroxyphenylalanine by Tyrosine Hydroxylase cDNAâTransfected C6 Cells: Application for Intracerebral Grafting. Journal of Neurochemistry, 53(3), 728â732. Portico.

Authors 9

Kohichi Uchida (first)
Ken Takamatsu (additional)
Norio Kaneda (additional)
Shigeo Toya (additional)
Yasuzo Tsukada (additional)
Yoshikazu Kurosawa (additional)
Keisuke Fujita (additional)
Toshiharu Nagatsu (additional)
Shinichi Kohsaka (additional)

References 26 Referenced 42

10.3171/jns.1985.62.2.0169
10.1126/science.161.3839.370
10.1111/j.1749-6632.1985.tb20799.x
10.1016/0006-8993(80)90692-7
10.1111/j.1471-4159.1986.tb02849.x
10.1111/j.1471-4159.1988.tb01088.x
10.1016/0003-2697(85)90529-9
10.1016/S0304-3940(85)80122-1
10.1073/pnas.75.10.4744
10.1016/0006-291X(87)90742-X
10.1016/0006-8993(86)91466-6
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Lacmmli U. K. (1970)
10.1056/NEJM198704023161402
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10.4049/jimmunol.139.7.2275
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{'key': 'e_1_2_2_22_1', 'first-page': '209', 'article-title': 'A role of calcium in the activation of a new protein kinase system', 'volume': '9', 'author': 'Nishizuka Y.', 'year': '1978', 'journal-title': 'Adv. Cyclic Nucleotide Res'} / Adv. Cyclic Nucleotide Res / A role of calcium in the activation of a new protein kinase system by Nishizuka Y. (1978)
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10.1111/j.1432-1033.1983.tb07319.x

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:22 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 1:06 p.m.)
Indexed	1 month, 4 weeks ago (July 6, 2025, 1:26 p.m.)
Issued	36 years ago (Sept. 1, 1989)
Published	36 years ago (Sept. 1, 1989)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	36 years ago (Sept. 1, 1989)

Funders 0

None

BibTeX

@article{Uchida_1989, title={Synthesis of <scp>l</scp>‐3,4‐Bihydroxyphenylalanine by Tyrosine Hydroxylase cDNA‐Transfected C6 Cells: Application for Intracerebral Grafting}, volume={53}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1989.tb11765.x}, DOI={10.1111/j.1471-4159.1989.tb11765.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Uchida, Kohichi and Takamatsu, Ken and Kaneda, Norio and Toya, Shigeo and Tsukada, Yasuzo and Kurosawa, Yoshikazu and Fujita, Keisuke and Nagatsu, Toshiharu and Kohsaka, Shinichi}, year={1989}, month=sep, pages={728–732} }

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