DOI: 10.1111/j.1471-4159.1988.tb03076.x. Nerve Growth Factor Increases the Intracellular Content of Acetylcholine in Cultured Septal Neurons from Developing Rats

Nerve Growth Factor Increases the Intracellular Content of Acetylcholine in Cultured Septal Neurons from Developing Rats

10.1111/j.1471-4159.1988.tb03076.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: The effects of nerve growth factor (NGF) on the intracellular content of acetylcholine (ACh) in cultured septal neurons from developing rats have been examined. The content of ACh could be measured by using HPLC and electrochemical detection (HPLC‐ECD), coupled with an immobilized enzyme column. This method of determination is very simple and rapid, and is highly sensitive. The content of ACh and the activity of choline acetyltransferase (ChAT) in cultured postnatal day 1 (PI) septal neurons grown on an astroglial “feeder” layer was increased during the period of cultivation by the addition of NGF. The activities of ChAT and the content of ACh increased in a dose‐dependent manner in direct relationship to the different amounts of NGF employed. These effects of NGF, i.e., elevating the intracellular content of ACh, accompanied by an increase in activity of ChAT, also were confirmed in the PI septal organotypic cultures. Additionally, embryonic day 17 (E17) septal neurons in a serum‐free medium displayed a similar responsiveness to NGF with respect to the elevation in the content of ACh and the increase in activity of ChAT. These results suggest that intracellular levels of ACh are likely to be regulated by NGF in a fashion similar to that of the activity levels of the biosynthetic enzyme.

Bibliography

Takei, N., Tsukui, H., & Hatanaka, H. (1988). Nerve Growth Factor Increases the Intracellular Content of Acetylcholine in Cultured Septal Neurons from Developing Rats. Journal of Neurochemistry, 51(4), 1118â1125. Portico.

Authors 3

Nobuyuki Takei (first)
Hiroko Tsukui (additional)
Hiroshi Hatanaka (additional)

References 39 Referenced 40

{'key': 'e_1_2_2_2_1', 'first-page': '49', 'article-title': 'A simple, reliable and rapid method for increasing the responsiveness of the glassy carbon electrode (GCE) for the analysis of biogenic amines by high performance liquid chro‐matography with electrochemical detection (LCEC).', 'volume': '31', 'author': 'Anton A. H.', 'year': '1984', 'journal-title': 'Life Sci'} / Life Sci / A simple, reliable and rapid method for increasing the responsiveness of the glassy carbon electrode (GCE) for the analysis of biogenic amines by high performance liquid chro‐matography with electrochemical detection (LCEC). by Anton A. H. (1984)
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{'key': 'e_1_2_2_34_1', 'first-page': '23', 'article-title': 'The localization and distribution of high affinity β‐nerve growth factor binding sites in the central nervous system of the adult rat. A light microscopic autoradiographic study using [125I]β‐nerve growth factor', 'volume': '20', 'author': 'Raivich G.', 'year': '1987', 'journal-title': 'Neu-roscience'} / Neu-roscience / The localization and distribution of high affinity β‐nerve growth factor binding sites in the central nervous system of the adult rat. A light microscopic autoradiographic study using [125I]β‐nerve growth factor by Raivich G. (1987)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:31 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 8:03 a.m.)
Indexed	10 hours, 48 minutes ago (Sept. 4, 2025, 10:01 a.m.)
Issued	36 years, 11 months ago (Oct. 1, 1988)
Published	36 years, 11 months ago (Oct. 1, 1988)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	36 years, 11 months ago (Oct. 1, 1988)

Funders 0

None

BibTeX

@article{Takei_1988, title={Nerve Growth Factor Increases the Intracellular Content of Acetylcholine in Cultured Septal Neurons from Developing Rats}, volume={51}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1988.tb03076.x}, DOI={10.1111/j.1471-4159.1988.tb03076.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Takei, Nobuyuki and Tsukui, Hiroko and Hatanaka, Hiroshi}, year={1988}, month=oct, pages={1118–1125} }

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