DOI: 10.1111/j.1471-4159.1988.tb01111.x. Effect of Ischemia on the In Vivo Release of Striatal Dopamine, Glutamate, and γ‐Aminobutyric Acid Studied by Intracerebral Microdialysis

Effect of Ischemia on the In Vivo Release of Striatal Dopamine, Glutamate, and γ‐Aminobutyric Acid Studied by Intracerebral Microdialysis

10.1111/j.1471-4159.1988.tb01111.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: We have previously described a marked attenuation of postischemic striatal neuronal death by prior substantia nigra (SN) lesioning. The present study was carried out to evaluate whether the protective effect of the lesion involves changes in the degree of local cerebral blood flow (1CBF) reduction, energy metabolite depletion, or alterations in the extracellular release of striatal dopamine (DA), glutamate (Glu), or γ‐aminobutyric acid (GABA). Control and SN‐lesioned rats were subjected to 20 min of forebrain ischemia by four‐vessel occlusion combined with systemic hypotension. Levels of 1CBF, as measured by the autoradiographic method, and energy metabolites were uniformly reduced in both the ipsi‐ and contralateral striata at the end of the ischemic period, a finding implying that the lesion did not affect the severity of the ischemic insult itself. Extracellular neurotransmitter levels were measured by microdialysis; the perfusate was collected before, during, and after ischemia. An ∼ 500‐fold increase in DA content, a 7‐fold increase in Giu content, and a 5‐fold increase in GABA content were observed during ischemia in nonlesioned animals. These levels gradually returned to baseline by 30 min of reperfusion. In SN‐lesioned rats, the release of DA was completely prevented, the release of GABA was not affected, and the release of Glu was partially attenuated. However, excessive extracellular Glu concentrations were still attained, which are potentially toxic. This, taken together with the previous neuropathological findings, suggests that excessive release of DA is important for the development of ischemic cell damage in the striatum.

Bibliography

Globus, M. Y. âT., Busto, R., Dietrich, W. D., Martinez, E., Valdes, I., & Ginsberg, M. D. (1988). Effect of Ischemia on the In Vivo Release of Striatal Dopamine, Glutamate, and Î³âAminobutyric Acid Studied by Intracerebral Microdialysis. Journal of Neurochemistry, 51(5), 1455â1464. Portico.

Authors 6

Mordecai Y.‐T. Globus (first)
Raul Busto (additional)
W. Dalton Dietrich (additional)
Elena Martinez (additional)
Isabel Valdes (additional)
Myron D. Ginsberg (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:20 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 11:54 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 1:25 a.m.)
Issued	36 years, 9 months ago (Nov. 1, 1988)
Published	36 years, 9 months ago (Nov. 1, 1988)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	36 years, 9 months ago (Nov. 1, 1988)

Funders 0

None

BibTeX

@article{Globus_1988, title={Effect of Ischemia on the In Vivo Release of Striatal Dopamine, Glutamate, and γ‐Aminobutyric Acid Studied by Intracerebral Microdialysis}, volume={51}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1988.tb01111.x}, DOI={10.1111/j.1471-4159.1988.tb01111.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Globus, Mordecai Y.‐T. and Busto, Raul and Dietrich, W. Dalton and Martinez, Elena and Valdes, Isabel and Ginsberg, Myron D.}, year={1988}, month=nov, pages={1455–1464} }

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