DOI: 10.1111/j.1471-4159.1988.tb01079.x. Partial Sequence of MAP2 in the Region of a Shared Epitope with Alzheimer Neuronbrillary Tangles

Partial Sequence of MAP2 in the Region of a Shared Epitope with Alzheimer Neuronbrillary Tangles

10.1111/j.1471-4159.1988.tb01079.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: A 3.3‐kilobase DNA complementary to human microtubule‐associated protein 2 (MAP2) was sequenced by the dideoxy method. The 3’end terminates at an internal EcoRI site before the polyA tail. Due to the arrangement of the cDNA insert in the λX gt 11 vector, the MAP2 fragment is not fused to β‐galactosidase when expressed. The Chou Fasman algorithm for the initial 58 amino acids from the first in‐frame methionine predicts an α helix. Beyond this point, a series of turns is predicted until amino acid 160. The frequent presence of basic residues in proximity to serines or threonines is consistent with multiple phosphorylation sites. The minimum specificity determinant for Ca2+/calmodulin‐dependent kinase is repeated 13 times. The sequence of a region containing a MAP2 epitope that is shared with the Alzheimer neuronbrillary tangle was determined by DNase treatment of the cDNA and antibody selecting the small resultant clones in a λ gt 11 sublibrary. Likewise, a MAP2 epitope that is not shared with the neurofibrillary tangle also has been located. Both epitopes are in the projection portion of the molecule. A bovine MAP2 cyanogen bromide fragment, which contains the epitope shared with the neurofibrillary tangle, is partially insoluble under aqueous conditions, probably due to the aggregation of oppositely charged residues. Thus, rapid cleavage of MAP2 to small peptides is probably necessary in vivo to prevent the aggregation of larger cleavage fragments.

Bibliography

Kosik, K. S., Orecchio, L. D., Bakalis, S., Duffy, L., & Neve, R. L. (1988). Partial Sequence of MAP2 in the Region of a Shared Epitope with Alzheimer Neuronbrillary Tangles. Journal of Neurochemistry, 51(2), 587â598. Portico.

Authors 5

Kenneth S. Kosik (first)
Lisa D. Orecchio (additional)
Shelley Bakalis (additional)
Lawrence Duffy (additional)
Rachael L. Neve (additional)

References 83 Referenced 46

{'key': 'e_1_2_2_2_1', 'first-page': '86a', 'article-title': 'MAPs mediate association of microtubules and neurofilaments in vitro [Abstract]', 'volume': '41', 'author': 'Aamodt E.', 'year': '1983', 'journal-title': 'Biophys. J'} / Biophys. J / MAPs mediate association of microtubules and neurofilaments in vitro [Abstract] by Aamodt E. (1983)
10.1016/0019-2791(75)90223-2
10.1083/jcb.96.6.1523
10.1083/jcb.98.1.320
10.1007/978-3-642-70644-8_13
10.1016/S0021-9258(19)70328-7 / J. Biol. Chem / Microtubule‐associated proteins from cultured HeLa cells by Bulinski J. C. (1980)
10.1111/j.1432-1033.1984.tb08235.x
10.1093/nar/14.14.5935
10.1126/science.6204383
10.1083/jcb.103.6.2739
{'key': 'e_1_2_2_12_1', 'article-title': 'Heterogeneity of microtubule‐associated protein (MAP2) in vertebrate brains.', 'author': 'Fischer I.', 'year': '1988', 'journal-title': 'Brain Res'} / Brain Res / Heterogeneity of microtubule‐associated protein (MAP2) in vertebrate brains. by Fischer I. (1988)
10.1073/pnas.83.17.6450
{'key': 'e_1_2_2_14_1', 'first-page': '363', 'volume-title': 'Molecular Cloning. A Laboratory Manual', 'author': 'Fritsch E. F.', 'year': '1982'} / Molecular Cloning. A Laboratory Manual by Fritsch E. F. (1982)
10.1083/jcb.106.3.779
10.1016/0014-5793(85)80357-4
10.1016/S0021-9258(19)68174-3 / J Biol. Chem / Phosphorylation by guanosine 3',5'‐monophosphate‐dependent protein kinase of synthetic peptide analogs of a site phosphorylated in histone H2B by Glass D. B. (1982)
10.1111/j.1471-4159.1985.tb04078.x
10.1016/0092-8674(84)90057-6
10.1083/jcb.101.5.1782
10.1523/JNEUROSCI.03-03-00557.1983 / J. Neurosci / Microtubule‐neurofilament segregation produced by β,β'‐iminodipropionitrile: evidence for the association of fast axonal transport with microtubules by Griffin J. W. (1983)
10.1083/jcb.78.3.958
10.1016/S0021-9258(18)34254-6 / J. Biol. Chem / The interaction of actin filaments with microtubules and microtubule‐associated proteins by Griffith L. (1982)
10.1016/S0021-9258(17)38495-8 / J. Biol Chem / Microtubule‐associated protein t: a component of Alzheimer paired helical filaments. by Grundke‐Iqbal I. (1986)
10.1016/S0021-9258(17)39001-4 / J. Biol. Chem / Microtubule‐associated proteins bind specifically to the 70 kDa neuro‐filament protein by Heimann R. (1985)
10.1111/j.1432-1033.1986.tb09356.x
10.1111/j.1471-4159.1987.tb13130.x
10.1111/j.1432-1033.1978.tb12716.x
10.1083/jcb.101.1.227
10.1038/321605a0
{'key': 'e_1_2_2_31_1', 'first-page': '49', 'volume-title': 'DNA Cloning Techniques: A Practical Approach', 'author': 'Huynh T.', 'year': '1984'} / DNA Cloning Techniques: A Practical Approach by Huynh T. (1984)
10.1021/bi00552a027
10.1016/0014-5793(84)81373-3
10.1016/S0021-9258(17)30119-9 / J. Biol. Chem / Phosphorylation of synthetic peptide analogs of rabbit cardiac troponin inhibitory subunit by the cyclic AMP‐dependent protein kinase by Kemp B. E. (1979)
10.1073/pnas.72.9.3448
10.1083/jcb.80.2.266
10.1073/pnas.81.24.7941
10.1073/pnas.83.11.4044
10.1002/ana.410220514
10.1146/annurev.bi.48.070179.004423
10.1016/0022-2836(82)90515-0
10.1038/227680a0
10.1083/jcb.95.3.982
10.1128/MCB.6.5.1529
10.1083/jcb.102.6.2098
10.1038/319463a0
10.1073/pnas.83.18.7013
10.1016/0076-6879(83)01005-8
10.1111/j.1432-1033.1983.tb07792.x
10.1002/j.1460-2075.1987.tb02409.x
10.1016/0169-328X(86)90011-2
10.1016/0169-328X(86)90033-1
10.1016/0378-1119(86)90372-0
10.1093/oxfordjournals.jbchem.a133510
10.1093/oxfordjournals.jbchem.a135625
10.1083/jcb.91.3.866
10.1083/jcb.95.2.672
10.1083/jcb.100.1.74
10.1016/S0021-9258(17)38593-9 / J. Biol. Chem / Substrate specificity of a multifunctional calmodulin‐depen‐dent protein kinase by Pearson R. B. (1985)
10.1073/pnas.66.3.1002
10.1016/0022-2836(80)90196-5
{'key': 'e_1_2_2_62_1', 'first-page': '250a', 'article-title': 'Associations between microtubules and coated vesicles in vitro [Abstract]', 'volume': '87', 'author': 'Sattilaro R. F.', 'year': '1980', 'journal-title': 'J. Cell Biol'} / J. Cell Biol / Associations between microtubules and coated vesicles in vitro [Abstract] by Sattilaro R. F. (1980)
10.1111/j.1432-1033.1982.tb07052.x
10.1016/S0006-3495(67)86579-2
10.1073/pnas.71.4.1342
10.1073/pnas.72.1.177
{'key': 'e_1_2_2_67_1', 'first-page': '657', 'volume-title': 'Biochemistry', 'author': 'Stryer L.', 'year': '1981'} / Biochemistry by Stryer L. (1981)
10.1073/pnas.76.9.4350
10.1016/S0021-9258(18)61047-6 / J. Biol. Chem / Numerous phosphates of microtubule‐associated protein 2 in living rat brain by Tsuyama S. (1987)
10.1073/pnas.77.6.3206
10.1083/jcb.92.2.435
{'key': 'e_1_2_2_72_1', 'volume-title': 'Modern Cell Biol.', 'author': 'Vallee R. B.', 'year': '1984'} / Modern Cell Biol. by Vallee R. B. (1984)
10.1016/S0021-9258(17)32839-9 / J. Biol. Chem / Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin by Vallee R. B. (1977)
10.1083/jcb.90.3.568
10.1016/S0022-5320(82)80051-8
10.1073/pnas.83.11.4040
10.1016/0014-5793(82)80042-2
10.1021/bi00278a020
10.1111/j.1471-4159.1985.tb12880.x
10.1016/0006-291X(82)92035-6
{'key': 'e_1_2_2_81_1', 'first-page': '81', 'article-title': "Alzheimer's neurofibrillary tangles contain unique epitopes and epitopes in common with the heat‐stable microtubule‐associated proteins, t and MAP2.", 'volume': '126', 'author': 'Yen S.‐H.', 'year': '1987', 'journal-title': 'Am. J. Pathol'} / Am. J. Pathol / Alzheimer's neurofibrillary tangles contain unique epitopes and epitopes in common with the heat‐stable microtubule‐associated proteins, t and MAP2. by Yen S.‐H. (1987)
10.1126/science.6356359
10.1016/0735-0651(85)90011-1
10.1016/0006-291X(76)90648-3

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 5:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 1:49 p.m.)
Indexed	11 months, 4 weeks ago (Sept. 7, 2024, 9:58 p.m.)
Issued	37 years, 1 month ago (Aug. 1, 1988)
Published	37 years, 1 month ago (Aug. 1, 1988)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	37 years, 1 month ago (Aug. 1, 1988)

Funders 0

None

BibTeX

@article{Kosik_1988, title={Partial Sequence of MAP2 in the Region of a Shared Epitope with Alzheimer Neuronbrillary Tangles}, volume={51}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1988.tb01079.x}, DOI={10.1111/j.1471-4159.1988.tb01079.x}, number={2}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Kosik, Kenneth S. and Orecchio, Lisa D. and Bakalis, Shelley and Duffy, Lawrence and Neve, Rachael L.}, year={1988}, month=aug, pages={587–598} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        8
      ]
    ],
    "date-time": "2024-09-08T01:58:16Z",
    "timestamp": 1725760696619
  },
  "reference-count": 83,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            10,
            5
          ]
        ],
        "date-time": "2006-10-05T00:00:00Z",
        "timestamp": 1160006400000
      },
      "content-version": "vor",
      "delay-in-days": 6639,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1988,
        8
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>Abstract: </jats:bold> A 3.3\u2010kilobase DNA complementary to human microtubule\u2010associated protein 2 (MAP2) was sequenced by the dideoxy method. The 3\u2019end terminates at an internal <jats:italic>Eco</jats:italic>RI site before the polyA tail. Due to the arrangement of the cDNA insert in the \u03bbX gt 11 vector, the MAP2 fragment is not fused to \u03b2\u2010galactosidase when expressed. The Chou Fasman algorithm for the initial 58 amino acids from the first in\u2010frame methionine predicts an \u03b1 helix. Beyond this point, a series of turns is predicted until amino acid 160. The frequent presence of basic residues in proximity to serines or threonines is consistent with multiple phosphorylation sites. The minimum specificity determinant for Ca<jats:sup>2+</jats:sup>/calmodulin\u2010dependent kinase is repeated 13 times. The sequence of a region containing a MAP2 epitope that is shared with the Alzheimer neuronbrillary tangle was determined by DNase treatment of the cDNA and antibody selecting the small resultant clones in a \u03bb gt 11 sublibrary. Likewise, a MAP2 epitope that is not shared with the neurofibrillary tangle also has been located. Both epitopes are in the projection portion of the molecule. A bovine MAP2 cyanogen bromide fragment, which contains the epitope shared with the neurofibrillary tangle, is partially insoluble under aqueous conditions, probably due to the aggregation of oppositely charged residues. Thus, rapid cleavage of MAP2 to small peptides is probably necessary in vivo to prevent the aggregation of larger cleavage fragments.</jats:p>",
  "DOI": "10.1111/j.1471-4159.1988.tb01079.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T21:57:14Z",
    "timestamp": 1160085434000
  },
  "page": "587-598",
  "source": "Crossref",
  "is-referenced-by-count": 46,
  "title": "Partial Sequence of MAP2 in the Region of a Shared Epitope with Alzheimer Neuronbrillary Tangles",
  "prefix": "10.1111",
  "volume": "51",
  "author": [
    {
      "given": "Kenneth S.",
      "family": "Kosik",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Lisa D.",
      "family": "Orecchio",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Shelley",
      "family": "Bakalis",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lawrence",
      "family": "Duffy",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Rachael L.",
      "family": "Neve",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_1",
      "first-page": "86a",
      "article-title": "MAPs mediate association of microtubules and neurofilaments in vitro [Abstract]",
      "volume": "41",
      "author": "Aamodt E.",
      "year": "1983",
      "journal-title": "Biophys. J"
    },
    {
      "key": "e_1_2_2_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0019-2791(75)90223-2"
    },
    {
      "key": "e_1_2_2_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.96.6.1523"
    },
    {
      "key": "e_1_2_2_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.98.1.320"
    },
    {
      "key": "e_1_2_2_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-642-70644-8_13"
    },
    {
      "key": "e_1_2_2_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "11570",
      "DOI": "10.1016/S0021-9258(19)70328-7",
      "article-title": "Microtubule\u2010associated proteins from cultured HeLa cells",
      "volume": "255",
      "author": "Bulinski J. C.",
      "year": "1980",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1984.tb08235.x"
    },
    {
      "key": "e_1_2_2_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/14.14.5935"
    },
    {
      "key": "e_1_2_2_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.6204383"
    },
    {
      "key": "e_1_2_2_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.103.6.2739"
    },
    {
      "key": "e_1_2_2_12_1",
      "article-title": "Heterogeneity of microtubule\u2010associated protein (MAP2) in vertebrate brains.",
      "author": "Fischer I.",
      "year": "1988",
      "journal-title": "Brain Res"
    },
    {
      "key": "e_1_2_2_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.83.17.6450"
    },
    {
      "key": "e_1_2_2_14_1",
      "first-page": "363",
      "volume-title": "Molecular Cloning. A Laboratory Manual",
      "author": "Fritsch E. F.",
      "year": "1982"
    },
    {
      "key": "e_1_2_2_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.106.3.779"
    },
    {
      "key": "e_1_2_2_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(85)80357-4"
    },
    {
      "key": "e_1_2_2_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "1196",
      "DOI": "10.1016/S0021-9258(19)68174-3",
      "article-title": "Phosphorylation by guanosine 3',5'\u2010monophosphate\u2010dependent protein kinase of synthetic peptide analogs of a site phosphorylated in histone H2B",
      "volume": "257",
      "author": "Glass D. B.",
      "year": "1982",
      "journal-title": "J Biol. Chem"
    },
    {
      "key": "e_1_2_2_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1985.tb04078.x"
    },
    {
      "key": "e_1_2_2_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90057-6"
    },
    {
      "key": "e_1_2_2_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.101.5.1782"
    },
    {
      "key": "e_1_2_2_21_1",
      "doi-asserted-by": "crossref",
      "first-page": "557",
      "DOI": "10.1523/JNEUROSCI.03-03-00557.1983",
      "article-title": "Microtubule\u2010neurofilament segregation produced by \u03b2,\u03b2'\u2010iminodipropionitrile: evidence for the association of fast axonal transport with microtubules",
      "volume": "3",
      "author": "Griffin J. W.",
      "year": "1983",
      "journal-title": "J. Neurosci"
    },
    {
      "key": "e_1_2_2_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.78.3.958"
    },
    {
      "key": "e_1_2_2_23_1",
      "doi-asserted-by": "crossref",
      "first-page": "9143",
      "DOI": "10.1016/S0021-9258(18)34254-6",
      "article-title": "The interaction of actin filaments with microtubules and microtubule\u2010associated proteins",
      "volume": "257",
      "author": "Griffith L.",
      "year": "1982",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_24_1",
      "doi-asserted-by": "crossref",
      "first-page": "6084",
      "DOI": "10.1016/S0021-9258(17)38495-8",
      "article-title": "Microtubule\u2010associated protein t: a component of Alzheimer paired helical filaments.",
      "volume": "261",
      "author": "Grundke\u2010Iqbal I.",
      "year": "1986",
      "journal-title": "J. Biol Chem"
    },
    {
      "key": "e_1_2_2_25_1",
      "doi-asserted-by": "crossref",
      "first-page": "12160",
      "DOI": "10.1016/S0021-9258(17)39001-4",
      "article-title": "Microtubule\u2010associated proteins bind specifically to the 70 kDa neuro\u2010filament protein",
      "volume": "260",
      "author": "Heimann R.",
      "year": "1985",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1986.tb09356.x"
    },
    {
      "key": "e_1_2_2_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1987.tb13130.x"
    },
    {
      "key": "e_1_2_2_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1978.tb12716.x"
    },
    {
      "key": "e_1_2_2_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.101.1.227"
    },
    {
      "key": "e_1_2_2_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/321605a0"
    },
    {
      "key": "e_1_2_2_31_1",
      "first-page": "49",
      "volume-title": "DNA Cloning Techniques: A Practical Approach",
      "author": "Huynh T.",
      "year": "1984"
    },
    {
      "key": "e_1_2_2_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00552a027"
    },
    {
      "key": "e_1_2_2_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(84)81373-3"
    },
    {
      "key": "e_1_2_2_34_1",
      "doi-asserted-by": "crossref",
      "first-page": "2638",
      "DOI": "10.1016/S0021-9258(17)30119-9",
      "article-title": "Phosphorylation of synthetic peptide analogs of rabbit cardiac troponin inhibitory subunit by the cyclic AMP\u2010dependent protein kinase",
      "volume": "254",
      "author": "Kemp B. E.",
      "year": "1979",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.72.9.3448"
    },
    {
      "key": "e_1_2_2_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.80.2.266"
    },
    {
      "key": "e_1_2_2_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.81.24.7941"
    },
    {
      "key": "e_1_2_2_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.83.11.4044"
    },
    {
      "key": "e_1_2_2_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ana.410220514"
    },
    {
      "key": "e_1_2_2_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.48.070179.004423"
    },
    {
      "key": "e_1_2_2_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(82)90515-0"
    },
    {
      "key": "e_1_2_2_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227680a0"
    },
    {
      "key": "e_1_2_2_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.95.3.982"
    },
    {
      "key": "e_1_2_2_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.6.5.1529"
    },
    {
      "key": "e_1_2_2_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.102.6.2098"
    },
    {
      "key": "e_1_2_2_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/319463a0"
    },
    {
      "key": "e_1_2_2_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.83.18.7013"
    },
    {
      "key": "e_1_2_2_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(83)01005-8"
    },
    {
      "key": "e_1_2_2_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1983.tb07792.x"
    },
    {
      "key": "e_1_2_2_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1987.tb02409.x"
    },
    {
      "key": "e_1_2_2_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0169-328X(86)90011-2"
    },
    {
      "key": "e_1_2_2_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0169-328X(86)90033-1"
    },
    {
      "key": "e_1_2_2_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(86)90372-0"
    },
    {
      "key": "e_1_2_2_54_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.jbchem.a133510"
    },
    {
      "key": "e_1_2_2_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.jbchem.a135625"
    },
    {
      "key": "e_1_2_2_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.91.3.866"
    },
    {
      "key": "e_1_2_2_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.95.2.672"
    },
    {
      "key": "e_1_2_2_58_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.100.1.74"
    },
    {
      "key": "e_1_2_2_59_1",
      "doi-asserted-by": "crossref",
      "first-page": "14471",
      "DOI": "10.1016/S0021-9258(17)38593-9",
      "article-title": "Substrate specificity of a multifunctional calmodulin\u2010depen\u2010dent protein kinase",
      "volume": "260",
      "author": "Pearson R. B.",
      "year": "1985",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_60_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.66.3.1002"
    },
    {
      "key": "e_1_2_2_61_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(80)90196-5"
    },
    {
      "key": "e_1_2_2_62_1",
      "first-page": "250a",
      "article-title": "Associations between microtubules and coated vesicles in vitro [Abstract]",
      "volume": "87",
      "author": "Sattilaro R. F.",
      "year": "1980",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "e_1_2_2_63_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1982.tb07052.x"
    },
    {
      "key": "e_1_2_2_64_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(67)86579-2"
    },
    {
      "key": "e_1_2_2_65_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.71.4.1342"
    },
    {
      "key": "e_1_2_2_66_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.72.1.177"
    },
    {
      "key": "e_1_2_2_67_1",
      "first-page": "657",
      "volume-title": "Biochemistry",
      "author": "Stryer L.",
      "year": "1981"
    },
    {
      "key": "e_1_2_2_68_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.9.4350"
    },
    {
      "key": "e_1_2_2_69_1",
      "doi-asserted-by": "crossref",
      "first-page": "10886",
      "DOI": "10.1016/S0021-9258(18)61047-6",
      "article-title": "Numerous phosphates of microtubule\u2010associated protein 2 in living rat brain",
      "volume": "262",
      "author": "Tsuyama S.",
      "year": "1987",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_70_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.6.3206"
    },
    {
      "key": "e_1_2_2_71_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.92.2.435"
    },
    {
      "key": "e_1_2_2_72_1",
      "volume-title": "Modern Cell Biol.",
      "author": "Vallee R. B.",
      "year": "1984"
    },
    {
      "key": "e_1_2_2_73_1",
      "doi-asserted-by": "crossref",
      "first-page": "377",
      "DOI": "10.1016/S0021-9258(17)32839-9",
      "article-title": "Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin",
      "volume": "252",
      "author": "Vallee R. B.",
      "year": "1977",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "e_1_2_2_74_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.90.3.568"
    },
    {
      "key": "e_1_2_2_75_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(82)80051-8"
    },
    {
      "key": "e_1_2_2_76_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.83.11.4040"
    },
    {
      "key": "e_1_2_2_77_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(82)80042-2"
    },
    {
      "key": "e_1_2_2_78_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00278a020"
    },
    {
      "key": "e_1_2_2_79_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1985.tb12880.x"
    },
    {
      "key": "e_1_2_2_80_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(82)92035-6"
    },
    {
      "key": "e_1_2_2_81_1",
      "first-page": "81",
      "article-title": "Alzheimer's neurofibrillary tangles contain unique epitopes and epitopes in common with the heat\u2010stable microtubule\u2010associated proteins, t and MAP2.",
      "volume": "126",
      "author": "Yen S.\u2010H.",
      "year": "1987",
      "journal-title": "Am. J. Pathol"
    },
    {
      "key": "e_1_2_2_82_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.6356359"
    },
    {
      "key": "e_1_2_2_83_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0735-0651(85)90011-1"
    },
    {
      "key": "e_1_2_2_84_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(76)90648-3"
    }
  ],
  "container-title": "Journal of Neurochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.1988.tb01079.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.1988.tb01079.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        21
      ]
    ],
    "date-time": "2023-10-21T17:49:03Z",
    "timestamp": 1697910543000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1988.tb01079.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1988,
        8
      ]
    ]
  },
  "references-count": 83,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1988,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1471-4159.1988.tb01079.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1471-4159.1988.tb01079.x",
  "relation": {},
  "ISSN": [
    "0022-3042",
    "1471-4159"
  ],
  "subject": [],
  "container-title-short": "Journal of Neurochemistry",
  "published": {
    "date-parts": [
      [
        1988,
        8
      ]
    ]
  }
}