DOI: 10.1111/j.1471-4159.1987.tb13123.x. Cellular Origins of Cyclic GMP Responses to Excitatory Amino Acid Receptor Agonists in Rat Cerebellum In Vitro

Cellular Origins of Cyclic GMP Responses to Excitatory Amino Acid Receptor Agonists in Rat Cerebellum In Vitro

10.1111/j.1471-4159.1987.tb13123.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Incubated slices and freshly dissociated cells from 8‐day‐old rat cerebellum were used to try to identify the cells that participate in the large increases in cyclic GMP levels that follow activation of excitatory amino acid receptors in this tissue. In the slices, cyclic GMP responses to L‐gluta‐mate and related excitants were unaffected by tetrodotoxin and could be replicated by the guanylate cyclase activator nitroprusside. Nitroprusside and the receptor agonists appeared to activate the same pool of the enzyme. Prior destruction of neuroblasts, deep nuclei, or Golgi neurones did not cause loss of responses to L‐glutamate. If granule cells were rendered necrotic, however, the cyclic GMP responses to all excitants tested were reduced by ≧ 90%. Substantial losses of responses to veratridine and high K+ levels also occurred, but the nitroprusside‐induced elevations were unaffected. In dissociated cell suspensions, the magnitude of responses to receptor agonists, but not those to nitroprusside, was markedly dependent on cell concentration. Responses to L‐glutamate were the same in cell suspensions that were Purkinje cell depleted and Purkinje cell enriched. It is concluded that granule cells are primarily involved in the cyclic GMP responses to excitatory amino acids but that the cyclic GMP accumulations occur elsewhere, probably in glial cells.

Bibliography

Garthwaite, J., & Garthwaite, G. (1987). Cellular Origins of Cyclic GMP Responses to Excitatory Amino Acid Receptor Agonists in Rat Cerebellum In Vitro. Journal of Neurochemistry, 48(1), 29â39. Portico.

Authors 2

J. Garthwaite (first)
G. Garthwaite (additional)

References 40 Referenced 181

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{'key': 'e_1_2_2_9_1', 'first-page': '463', 'article-title': 'Characterization of immunofluorescent cyclic GMP‐positive fibres in the central nervous system', 'volume': '5', 'author': 'Cumming R.', 'year': '1979', 'journal-title': 'J. Cyclic Nucleotide Res.'} / J. Cyclic Nucleotide Res. / Characterization of immunofluorescent cyclic GMP‐positive fibres in the central nervous system by Cumming R. (1979)
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{'key': 'e_1_2_2_11_1', 'first-page': '373', 'article-title': 'Cyclic nucleotides in the nervous system', 'volume': '15', 'author': 'Drummond G. I.', 'year': '1983', 'journal-title': 'Adv. Cyclic Nudeotide Res.'} / Adv. Cyclic Nudeotide Res. / Cyclic nucleotides in the nervous system by Drummond G. I. (1983)
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{'key': 'e_1_2_2_30_1', 'first-page': '225', 'volume-title': 'Handbook of Experimental Pharmacology, Vol. 58, Pan I: Cyclic Nucleotides I', 'author': 'Mittal C. K.', 'year': '1982'} / Handbook of Experimental Pharmacology, Vol. 58, Pan I: Cyclic Nucleotides I by Mittal C. K. (1982)
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Dates

Type	When
Created	18 years, 8 months ago (Dec. 13, 2006, 6:35 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 7:43 p.m.)
Indexed	2 days, 3 hours ago (Sept. 4, 2025, 9:24 a.m.)
Issued	38 years, 8 months ago (Jan. 1, 1987)
Published	38 years, 8 months ago (Jan. 1, 1987)
Published Online	18 years, 8 months ago (Dec. 12, 2006)
Published Print	38 years, 8 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{Garthwaite_1987, title={Cellular Origins of Cyclic GMP Responses to Excitatory Amino Acid Receptor Agonists in Rat Cerebellum In Vitro}, volume={48}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1987.tb13123.x}, DOI={10.1111/j.1471-4159.1987.tb13123.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Garthwaite, J. and Garthwaite, G.}, year={1987}, month=jan, pages={29–39} }

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