DOI: 10.1111/j.1471-4159.1987.tb05636.x. Localization of Phospholipid Synthesis to Schwann Cells and Axons

Localization of Phospholipid Synthesis to Schwann Cells and Axons

10.1111/j.1471-4159.1987.tb05636.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Quantitative electron microscopic autoradiography was used to detect and characterize endoneurial sites of lipid synthesis in mouse sciatic nerve. Six tritiated phospholipid precursors (choline, serine, methionine, inositol, glyc‐erol, and ethanolamine) and a protein precursor (proline) were individually injected into exposed nerves and after 2 h the mice were perfused with buffered aldehyde. The labeled segments of nerve were prepared for autoradiography with procedures that selectively remove nonincorporated precursors and other aqueous metabolites, while preserving nerve lipids (and proteins). At both the light and electron microscope levels, the major site of phospholipid and protein synthesis was the crescent‐shaped perinuclear cytoplasm of my‐elinating Schwann cells. Other internodal Schwann cell cytoplasm, including that in surface channels, Schmidt‐Lanterman incisures, and paranodal regions, was less well labeled than the perinuclear region. Newly formed proteins were selectively located in the Schwann cell nucleus. Lipid and protein formation was also detected in unmyelinated fiber bundles and in endoneurial and perineurial cells. Tritiated inositol was selectively incorporated into phospholip‐ids in both myelinated axons and unmyelinated fibers. Like inositol, glycerol incorporation appeared particularly active in unmyelinated fibers. Quantitative autoradiographic analyses substantiated the following points: (1) myelinating Schwann cells dominate phospholipid and protein synthesis, (2) myelinated axons selectively incorporate tritiated inositol, (3) phospholipid precursors label myelin sheaths and myelinated axons better than proline.

Bibliography

Gould, R. M., Holshek, J., Silverman, W., & Spivack, W. D. (1987). Localization of Phospholipid Synthesis to Schwann Cells and Axons. Journal of Neurochemistry, 48(4), 1121â1131. Portico.

Authors 4

Robert M. Gould (first)
John Holshek (additional)
Wayne Silverman (additional)
Warren D. Spivack (additional)

References 31 Referenced 27

10.1007/978-1-4757-1830-0_7
{'key': 'e_1_2_2_3_1', 'first-page': '3', 'volume-title': 'Physiology and Pathobiology of Axons', 'author': 'Berthold C.‐H', 'year': '1978'} / Physiology and Pathobiology of Axons by Berthold C.‐H (1978)
10.1016/S0070-2161(08)60468-2
10.1016/0006-8993(80)91094-X
10.1016/0006-8993(76)90569-2
10.1083/jcb.75.2.326
10.1083/jcb.68.3.480
10.1007/BF01257964
{'key': 'e_1_2_2_10_1', 'first-page': '397', 'volume-title': 'The Schwann cell, in Handbook of Neurochemistry', 'author': 'Gould R. M.', 'year': '1982'} / The Schwann cell, in Handbook of Neurochemistry by Gould R. M. (1982)
{'key': 'e_1_2_2_11_1', 'first-page': '221', 'volume-title': 'Phospholipids of the Nervous System, Vol. 1: Metabolism', 'author': 'Gould R. M.', 'year': '1982'} / Phospholipids of the Nervous System, Vol. 1: Metabolism by Gould R. M. (1982)
10.1111/j.1471-4159.1983.tb13569.x
10.1111/j.1471-4159.1983.tb13570.x
10.1111/j.1471-4159.1987.tb05595.x
10.1152/physrev.1980.60.4.1167
{'key': 'e_1_2_2_16_1', 'volume-title': 'Basic Electron Microscopy Techniques', 'author': 'Hayat M. S.', 'year': '1972'} / Basic Electron Microscopy Techniques by Hayat M. S. (1972)
10.1016/S0074-7696(08)62426-2
10.1007/BF00491492
10.1016/0006-8993(80)90978-6
{'key': 'e_1_2_2_20_1', 'first-page': '25', 'article-title': 'Structure of normal peripheral myelinated nerve fibres', 'volume': '31', 'author': 'Landon D. N.', 'year': '1981', 'journal-title': 'Adv. Neurol.'} / Adv. Neurol. / Structure of normal peripheral myelinated nerve fibres by Landon D. N. (1981)
10.1111/j.1471-4159.1968.tb08953.x
{'key': 'e_1_2_2_22_1', 'first-page': '501', 'article-title': 'Cell‐to‐cell transfer of glial proteins to the squid giant axon', 'volume': '79', 'author': 'Lasek R. J.', 'year': '1977', 'journal-title': 'The glia-neuron protein transfer hypothesis. J. Cell Biol.'} / The glia-neuron protein transfer hypothesis. J. Cell Biol. / Cell‐to‐cell transfer of glial proteins to the squid giant axon by Lasek R. J. (1977)
{'key': 'e_1_2_2_23_1', 'first-page': '135', 'volume-title': 'Phospholipids of the Nervous System', 'author': 'Ledeen R. W.', 'year': '1985'} / Phospholipids of the Nervous System by Ledeen R. W. (1985)
10.1111/j.1471-4159.1981.tb05304.x
{'key': 'e_1_2_2_25_1', 'volume-title': 'Techniques of Autoradiography', 'author': 'Rogers A. W.', 'year': '1979'} / Techniques of Autoradiography by Rogers A. W. (1979)
10.1083/jcb.41.1.1
10.1002/9780470719633.ch12
10.1016/0006-8993(84)90032-5
{'key': 'e_1_2_2_29_1', 'first-page': '219', 'volume-title': 'Advances in Optical and Electron Microscopy, Vol. 3', 'author': 'Williams M. A.', 'year': '1969'} / Advances in Optical and Electron Microscopy, Vol. 3 by Williams M. A. (1969)
{'key': 'e_1_2_2_30_1', 'first-page': '126', 'volume-title': 'Techniques in Protein Biosynthesis, Vol. 3', 'author': 'Williams M. A.', 'year': '1973'} / Techniques in Protein Biosynthesis, Vol. 3 by Williams M. A. (1973)
{'key': 'e_1_2_2_31_1', 'first-page': '85', 'volume-title': 'Practical Methods in Electron Microscopy, Vol. 6', 'author': 'Williams M. A.', 'year': '1977'} / Practical Methods in Electron Microscopy, Vol. 6 by Williams M. A. (1977)
10.1037/11774-000

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 11:53 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 10:26 p.m.)
Indexed	2 months, 2 weeks ago (June 12, 2025, 5:27 a.m.)
Issued	38 years, 4 months ago (April 1, 1987)
Published	38 years, 4 months ago (April 1, 1987)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	38 years, 4 months ago (April 1, 1987)

Funders 0

None

BibTeX

@article{Gould_1987, title={Localization of Phospholipid Synthesis to Schwann Cells and Axons}, volume={48}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1987.tb05636.x}, DOI={10.1111/j.1471-4159.1987.tb05636.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Gould, Robert M. and Holshek, John and Silverman, Wayne and Spivack, Warren D.}, year={1987}, month=apr, pages={1121–1131} }

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