DOI: 10.1111/j.1471-4159.1987.tb05634.x. Comparison of Tryptic Peptides of Benzodiazepine Binding Proteins Photolabeled with [<sup>3</sup>H]Flunitrazepam or [<sup>3</sup>H]Ro 15

Comparison of Tryptic Peptides of Benzodiazepine Binding Proteins Photolabeled with [3H]Flunitrazepam or [3H]Ro 15–4513

10.1111/j.1471-4159.1987.tb05634.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: When rat brain membranes were incubated with the benzodiazepine agonist [3H]flunitrazepam or the partial inverse benzodiazepine agonist [3H]Ro 15–4513 in the presence of ultraviolet light one protein (P51) was specifically and irreversibly labeled in cerebellum and at least two proteins (P51 and P55) were labeled in hippocampus. After digestion of the membranes with trypsin, protein P51 was degraded into several peptides. When P51 was photolabeled with [3H]Ro 15–4513, four peptides with apparent molecular weights of 39,000, 29,000, 21,000, and 17,000 were observed. When P51 was labeled with [3H]flunitrazepam, only two peptides with apparent molecular weights of 39,000 and 25,000 were obtained. Protein P55 was only partially degraded by trypsin, and whether it was labeled with [3H]‐flunitrazepam or [3H]Ro 15–4513 it yielded the same two proteolytic peptides with apparent molecular weights of 42,000 and 45,000. These results support the existence of at least two different benzodiazepine receptor subtypes associated with proteins P51 and P55. The different receptors seem to be differentially protected against treatment with trypsin. In addition, these results indicate that in the benzodiazepine receptor subtype associated with P51 benzodiazepine agonists and partial inverse benzodiazepine agonists irreversibly bind to different parts of the molecule.

Bibliography

Sieghart, W., Eichinger, A., & Zezula, J. (1987). Comparison of Tryptic Peptides of Benzodiazepine Binding Proteins Photolabeled with [3H]Flunitrazepam or [3H]Ro 15â4513. Journal of Neurochemistry, 48(4), 1109â1114. Portico.

Authors 3

W. Sieghart (first)
A. Eichinger (additional)
J. Zezula (additional)

References 15 Referenced 9

10.1111/j.1471-4159.1983.tb12672.x
10.1111/j.1471-4159.1985.tb05496.x
10.1016/0024-3205(84)90602-7
{'key': 'e_1_2_2_5_1', 'first-page': '431', 'volume-title': 'Pharmacology of Benzodiazepines', 'author': 'Lippa A. S.', 'year': '1982'} / Pharmacology of Benzodiazepines by Lippa A. S. (1982)
10.1111/j.1471-4159.1982.tb10848.x
10.1073/pnas.77.3.1666
10.1016/0014-2999(84)90358-3
10.1111/j.1471-4159.1983.tb11812.x
10.1038/286285a0
10.1016/0304-3940(82)90056-8
10.1016/0014-2999(82)90620-3
{'key': 'e_1_2_2_13_1', 'first-page': '291', 'article-title': 'Properties of [3H]flunitrazepam binding to different benzodiazepine binding proteins. Eur', 'volume': '88', 'author': 'Sieghart W.', 'year': '1983', 'journal-title': 'J. Pharmacol'} / J. Pharmacol / Properties of [3H]flunitrazepam binding to different benzodiazepine binding proteins. Eur by Sieghart W. (1983)
10.1111/j.1471-4159.1987.tb13125.x
{'key': 'e_1_2_2_15_1', 'first-page': '7219', 'article-title': 'A γ‐aminobutyric acid/benzodi‐azepine receptor complex from bovine cerebral cortex', 'volume': '259', 'author': 'Sigel E.', 'year': '1984', 'journal-title': 'Improved purification with preservation of regulatory sites and their interactions. J. Biol. Chem.'} / Improved purification with preservation of regulatory sites and their interactions. J. Biol. Chem. / A γ‐aminobutyric acid/benzodi‐azepine receptor complex from bovine cerebral cortex by Sigel E. (1984)
{'key': 'e_1_2_2_16_1', 'first-page': '6965', 'article-title': 'A γ‐aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex', 'volume': '258', 'author': 'Sigel E.', 'year': '1983', 'journal-title': 'Purification and partial characterization. J. Biol. Chem.'} / Purification and partial characterization. J. Biol. Chem. / A γ‐aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex by Sigel E. (1983)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 11:53 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 10:25 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 6:59 p.m.)
Issued	38 years, 5 months ago (April 1, 1987)
Published	38 years, 5 months ago (April 1, 1987)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	38 years, 5 months ago (April 1, 1987)

Funders 0

None

BibTeX

@article{Sieghart_1987, title={Comparison of Tryptic Peptides of Benzodiazepine Binding Proteins Photolabeled with [3H]Flunitrazepam or [3H]Ro 15–4513}, volume={48}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1987.tb05634.x}, DOI={10.1111/j.1471-4159.1987.tb05634.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Sieghart, W. and Eichinger, A. and Zezula, J.}, year={1987}, month=apr, pages={1109–1114} }

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