DOI: 10.1111/j.1471-4159.1987.tb05595.x. Phospholipid Metabolism in Mouse Sciatic Nerve In Vivo

Phospholipid Metabolism in Mouse Sciatic Nerve In Vivo

10.1111/j.1471-4159.1987.tb05595.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: To probe the activities of various pathways of lipid metabolism in peripheral nerve, six phospholipid‐directed precursors were individually injected into the exposed sciatic nerves of adult mice, and their incorporation into phos‐pholipids and proteins was studied over a 2‐week period. Tritiated choline, inositol, ethanolamine, serine, and glyc‐erol were mainly used in phospholipid synthesis; in contrast, methyl‐labeled methionine was primarily incorporated into protein. Phosphatidylcholine was the main lipid formed from tritiated choline, glycerol, and methionine precursors. Phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol were the main lipids formed from serine, ethanolamine, and inositol, respectively. With time there was a shift in label among phospholipids, with higher proportions of choline appearing in sphingomyelin, glycerol in phosphatidylserine, ethanolamine in phosphatidylethanolamine (plasmalogen), and inositol in polyphosphoinosi‐tides, especially phosphatidylinositol 4,5‐bisphosphate. We suggest that the delay in formation of these phospholipids, which are concentrated in peripheral nerve myelin, may, at least in part, be due to their formation at a site(s) distant from the sites where the bulk of Schwann cell lipids are made. We propose that separating the synthesis of these my‐elin‐destined lipids to near the Schwann cell's plasma membrane would facilitate their concentration in peripheral nerve myelin sheaths. At earlier labeling times, ethanolamine and glycerol were more actively incorporated into phosphatidylcholine and phosphatidylinositol, respectively, than later. The transient labeling of these phospholipids may reflect some unique role in peripheral nerve function.

Bibliography

Gould, R. M., Connell, F., & Spivack, W. (1987). Phospholipid Metabolism in Mouse Sciatic Nerve In Vivo. Journal of Neurochemistry, 48(3), 853â859. Portico.

Authors 3

Robert M. Gould (first)
Fred Connell (additional)
Warren Spivack (additional)

References 42 Referenced 15

10.1111/j.1471-4159.1967.tb09576.x
{'key': 'e_1_2_2_3_1', 'first-page': '1', 'volume-title': 'Phospholipids', 'author': 'Ansell G. B.', 'year': '1982'} / Phospholipids by Ansell G. B. (1982)
10.1016/0005-2760(84)90079-1
10.1016/B978-0-12-024919-0.50011-4
10.1007/978-1-4757-1830-0_7
10.1111/j.1471-4159.1984.tb00912.x
10.1111/j.1471-4159.1979.tb11741.x
10.1111/j.1471-4159.1983.tb00844.x
{'key': 'e_1_2_2_10_1', 'first-page': '227', 'article-title': 'Abnormal sciatic nerve myo‐inositol metabolism in the streptozotocin‐diabetic rat', 'volume': '29', 'author': 'Clements R. S.', 'year': '1980', 'journal-title': 'Di-abetes'} / Di-abetes / Abnormal sciatic nerve myo‐inositol metabolism in the streptozotocin‐diabetic rat by Clements R. S. (1980)
10.1111/j.1471-4159.1978.tb12417.x
{'key': 'e_1_2_2_12_1', 'first-page': '1661', 'article-title': 'Biosynthesis of sphingomyelin from phosphatidylcholine to erythro‐ceramide in a cell‐free system', 'volume': '354', 'author': 'Diringer H.', 'year': '1973', 'journal-title': 'Hoppe Seylers Z. Physiol. Chem.'} / Hoppe Seylers Z. Physiol. Chem. / Biosynthesis of sphingomyelin from phosphatidylcholine to erythro‐ceramide in a cell‐free system by Diringer H. (1973)
10.1111/j.1471-4159.1985.tb12908.x
10.1016/0006-8993(76)90569-2
10.1083/jcb.75.2.326
{'key': 'e_1_2_2_16_1', 'first-page': '321', 'volume-title': 'Phospholipids of the Nervous System', 'author': 'Gould R. M.', 'year': '1985'} / Phospholipids of the Nervous System by Gould R. M. (1985)
10.1083/jcb.68.3.480
10.1007/BF01257964
{'key': 'e_1_2_2_19_1', 'first-page': '397', 'volume-title': 'Handbook of Neurochemistry', 'author': 'Gould R. M.', 'year': '1982'} / Handbook of Neurochemistry by Gould R. M. (1982)
10.1111/j.1471-4159.1983.tb13569.x
10.1111/j.1471-4159.1983.tb13570.x
GouldR. M. HolshekJ. SilvermanW. andSpivackW. D.(1987)Localization of phospholipid synthesis to Schwann cells and axons.J. Neurochem. (in press). (10.1111/j.1471-4159.1987.tb05636.x)
10.1172/JCI108052
10.1111/j.1749-6632.1982.tb21415.x
10.1016/S0021-9258(19)42169-8 / J. Biol. Chem. / Phospholipid exchange between membranes by Helmkamp G. M. (1974)
10.1007/BF02532120
10.1007/978-1-4615-7748-5_9
10.1016/0006-8993(74)90356-4
{'key': 'e_1_2_2_29_1', 'first-page': '328', 'article-title': 'The phosphatidylinositol kinase of rat brain', 'volume': '101', 'author': 'Kai M.', 'year': '1966', 'journal-title': 'Biochemistry'} / Biochemistry / The phosphatidylinositol kinase of rat brain by Kai M. (1966)
10.1016/0006-8993(80)90978-6
10.1111/j.1471-4159.1970.tb02218.x
10.1083/jcb.100.1.27
10.1016/0005-2760(82)90114-X
10.1016/0005-2760(67)90053-7
10.1016/S0021-9258(18)61792-2 / J. Biol. Chem. / Molecular weight determination of pro‐tein‐dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system by Neville D. M. (1971)
10.1042/bj1670229
10.1016/0003-9861(83)90613-6
{'key': 'e_1_2_2_38_1', 'first-page': '133', 'volume-title': 'Handbook of Neurochemistry', 'author': 'Porcellati G.', 'year': '1983'} / Handbook of Neurochemistry by Porcellati G. (1983)
10.1007/BF02533407
10.1016/0006-8993(83)90410-9
10.1042/bj1000012 / Biochem. J. / The polyphosphoinosi‐tides and other lipids of peripheral nerves by Sheltawy A. (1966)
10.1016/S0021-9258(19)42911-6
10.1021/bi00540a027

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 8:07 a.m.)
Indexed	1 year, 1 month ago (July 20, 2024, 2:49 p.m.)
Issued	38 years, 6 months ago (March 1, 1987)
Published	38 years, 6 months ago (March 1, 1987)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	38 years, 6 months ago (March 1, 1987)

Funders 0

None

BibTeX

@article{Gould_1987, title={Phospholipid Metabolism in Mouse Sciatic Nerve In Vivo}, volume={48}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1987.tb05595.x}, DOI={10.1111/j.1471-4159.1987.tb05595.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Gould, Robert M. and Connell, Fred and Spivack, Warren}, year={1987}, month=mar, pages={853–859} }

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