DOI: 10.1111/j.1471-4159.1987.tb03410.x. Differential Effects of Nerve Growth Factor and Ciliary Neuronotrophic Factor on Catecholamine Storage and Catecholamine Synthesizing Enzymes of Cultured Rat Chromaffin Cells

Differential Effects of Nerve Growth Factor and Ciliary Neuronotrophic Factor on Catecholamine Storage and Catecholamine Synthesizing Enzymes of Cultured Rat Chromaffin Cells

10.1111/j.1471-4159.1987.tb03410.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: : The effects of nerve growth factor (NGF) and ciliary neuronotrophic factor (CNTF) on Catecholamine content and in vitro activities of tyrosine hydroxylase (TH) and phenylethanolamine N‐methyltransferase (PNMT) were studied in adrenal chromaffin cells cultured from 8‐day‐old rats. Both NGF and CNTF enhanced chromaffin cell survival and partially prevented losses of adrenaline during the 4‐day culture period in a dose‐dependent manner. CNTF was more potent, although cellular levels of adrenaline and noradrenaline were not maintained. NGF did not add to the effect of CNTF. The effect of CNTF on Catecholamine storage was not accompanied by changes in the activities of TH and PNMT. In contrast, NGF induced TH but not PNMT activity. These data indicate differences between the mechanisms by which NGF and CNTF affect adrenal chromaffin cells.

Bibliography

Seidl, K., Manthorpe, M., Varon, S., & Unsicker, K. (1987). Differential Effects of Nerve Growth Factor and Ciliary Neuronotrophic Factor on Catecholamine Storage and Catecholamine Synthesizing Enzymes of Cultured Rat Chromaffin Cells. Journal of Neurochemistry, 49(1), 169â174. Portico.

Authors 4

K. Seidl (first)
M. Manthorpe (additional)
S. Varon (additional)
K. Unsicker (additional)

References 46 Referenced 20

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10.1002/j.1460-2075.1982.tb01207.x
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{'key': 'e_1_2_2_15_1', 'first-page': '234', 'article-title': 'Tetanus toxin binding to different morphological phenotypes of cultured rat and bovine adrenal medullary cells', 'volume': '3', 'author': 'Lietzke R.', 'year': '1983', 'journal-title': 'Neurosci. Lett.'} / Neurosci. Lett. / Tetanus toxin binding to different morphological phenotypes of cultured rat and bovine adrenal medullary cells by Lietzke R. (1983)
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10.1016/S0021-9258(17)34353-3 / J. Biol. Chem. / Nerve growth factor‐mediated induction of tyrosine hydroxylase in rat superior cervical ganglia in vitro by Max S. R. (1978)
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10.1523/JNEUROSCI.03-07-01488.1983 / J. Neurosci. / Pressure microinjections of nerve growth factor and anti‐nerve growth factor into the nucleus and cytoplasm: lack of effects on neurite outgrowth from pheochromocytoma cells by Seeley P. J. (1983)
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SeidlK.(1986)Isolated embryonic chromaffin cells in culture: a model system for studying differentiation of neural crest derivatives.Ph.D. Thesis Faculty of Biology University of Marburg.
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{'key': 'e_1_2_2_41_1', 'article-title': 'Survival, morphology and catecholamine storage of chromaffin cells in serum‐free culture: evidence for a survival and differentiation promoting activity in medium conditioned by purified chromaffin cells', 'author': 'Unsicker K.', 'year': '1986', 'journal-title': 'Neurochem. Res.'} / Neurochem. Res. / Survival, morphology and catecholamine storage of chromaffin cells in serum‐free culture: evidence for a survival and differentiation promoting activity in medium conditioned by purified chromaffin cells by Unsicker K. (1986)
{'key': 'e_1_2_2_42_1', 'article-title': 'Chromaffin cells—a novel source for neuronotrophic factors. Exp. Brain Res. (Suppi: Histochem', 'author': 'Unsicker K.', 'year': '1986', 'journal-title': 'Cell Biol. Autonomic Neurons and Paraganglia)'} / Cell Biol. Autonomic Neurons and Paraganglia) / Chromaffin cells—a novel source for neuronotrophic factors. Exp. Brain Res. (Suppi: Histochem by Unsicker K. (1986)
{'key': 'e_1_2_2_43_1', 'first-page': '333', 'volume-title': 'Tissue Culture in Neurobiology', 'author': 'Varon S.', 'year': '1980'} / Tissue Culture in Neurobiology by Varon S. (1980)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:20 p.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 5:29 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:28 a.m.)
Issued	38 years, 2 months ago (July 1, 1987)
Published	38 years, 2 months ago (July 1, 1987)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	38 years, 2 months ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Seidl_1987, title={Differential Effects of Nerve Growth Factor and Ciliary Neuronotrophic Factor on Catecholamine Storage and Catecholamine Synthesizing Enzymes of Cultured Rat Chromaffin Cells}, volume={49}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1987.tb03410.x}, DOI={10.1111/j.1471-4159.1987.tb03410.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Seidl, K. and Manthorpe, M. and Varon, S. and Unsicker, K.}, year={1987}, month=jul, pages={169–174} }

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