DOI: 10.1111/j.1471-4159.1986.tb00718.x. Calcium‐Activated Neutral Proteinase of Human Brain: Subunit Structure and Enzymatic Properties of Multiple Molecular Forms

Calcium‐Activated Neutral Proteinase of Human Brain: Subunit Structure and Enzymatic Properties of Multiple Molecular Forms

10.1111/j.1471-4159.1986.tb00718.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract Calcium‐activated neutral proteinase (CANP) was purified 2,625‐fold from postmortem human cerebral cortex by a procedure involving chromatography on diethylaminoethyl (DEAE)‐cellulose, phenyl‐Sepharose, Ultrogel AcA‐44, and DEAE‐Biogel A. The major active form of CANP exhibited a molecular weight of 94–100 kilodaltons (Kd) by gel filtration on Sephacryl 300 and consisted of 78‐Kd and 27‐Kd subunits. Two‐dimensional gel electrophoresis resolved the small subunit into two molecular species with different isoelectric points. CANP degraded most human cytoskeletal proteins but was particularly active toward fodrin and the neurofilament protein subunits (145 Kd > 200 Kd > 70 Kd). The enzyme required 175 μMCa2+ for half‐maximal activation and 2 mM Ca2+ for optimal activity toward [methl‐14C]azocasein. Other divalent metal ions were poor activators of the enzyme, and some, including copper, lead, and zinc, strongly inhibited the enzyme. Aluminum, a neurotoxic ion that induces neurofilament accumulations in mammalian brain, inhibited the enzyme 47% at 1 mM and 100% at 5 mM A second CANP form lacking the 27‐Kd subunit was partially resolved from the 100‐Kd heterodimer during DEAE‐Biogel A chromatography. The 78‐Kd monomer exhibited the same specific activity, calcium ion requirement, pH optimum, and specificity for cytoskeletal proteins as the 100‐Kd heterodimer, suggesting that the 27‐Kd subunit is not essential for the major catalytic properties of the enzyme. The rapid autolysis of the 27‐Kd subunit to a 18‐Kd intermediate when CANP is exposed to calcium may explain differences between our results and previous reports, which describe brain mCANP in other species as a 76‐80‐Kd monomer or a heterodimer containing 76‐80‐Kd and 17‐20‐Kd subunits. The similarity of the 100‐Kd human brain CANP to CANPs in nonneural tissues indicates that the heterodimeric form is relatively conserved among various tissues and species.

Bibliography

Vitto, A., & Nixon, R. A. (1986). CalciumâActivated Neutral Proteinase of Human Brain: Subunit Structure and Enzymatic Properties of Multiple Molecular Forms. Journal of Neurochemistry, 47(4), 1039â1051. Portico.

Authors 2

Anthony Vitto (first)
Ralph A. Nixon (additional)

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Dates

Type	When
Created	15 years, 1 month ago (July 28, 2010, 10:30 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 6:23 p.m.)
Indexed	1 year, 5 months ago (March 20, 2024, 5:45 a.m.)
Issued	38 years, 11 months ago (Oct. 1, 1986)
Published	38 years, 11 months ago (Oct. 1, 1986)
Published Online	18 years, 11 months ago (Oct. 5, 2006)
Published Print	38 years, 11 months ago (Oct. 1, 1986)

Funders 0

None

BibTeX

@article{Vitto_1986, title={Calcium‐Activated Neutral Proteinase of Human Brain: Subunit Structure and Enzymatic Properties of Multiple Molecular Forms}, volume={47}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1986.tb00718.x}, DOI={10.1111/j.1471-4159.1986.tb00718.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Vitto, Anthony and Nixon, Ralph A.}, year={1986}, month=oct, pages={1039–1051} }

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