DOI: 10.1111/j.1471-4159.1985.tb12873.x. Cell‐Free Synthesis of the D2‐Cell Adhesion Molecule: Evidence for Three Primary Translation Products

Cell‐Free Synthesis of the D2‐Cell Adhesion Molecule: Evidence for Three Primary Translation Products

10.1111/j.1471-4159.1985.tb12873.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: The D2‐cell adhesion molecule (D2‐CAM) is a membrane glycoprotein that is involved in cell‐cell adhesion in the nervous system. To study the biosynthesis of D2‐CAM we have translated free and membrane‐bound polysomes from rat brain in vitro in the rabbit reticulocyte lysate system. D2‐CAM was exclusively synthesized on membrane‐bound polysomes. The primary translation products of D2‐CAM were three polypeptides of apparent molecular weights 187,000, 134,000, and 112,000. No interconversion between these polypeptides was detected. In contrast to previous suggestions, we conclude that all three D2‐CAM polypeptides are primary translation products. When translating polysomes from embryonic and postnatal rat brain, we found that the relative amounts of the three polypeptides synthesized varied with age. Their molecular weights, however, were not age‐dependent.

Bibliography

Hansen, O. C., Nybroe, O., & Bock, E. (1985). CellâFree Synthesis of the D2âCell Adhesion Molecule: Evidence for Three Primary Translation Products. Journal of Neurochemistry, 44(3), 712â717. Portico.

Authors 3

Ole C. Hansen (first)
Ole Nybroe (additional)
Elisabeth Bock (additional)

References 22 Referenced 21

{'key': 'e_1_2_2_2_1', 'volume-title': 'A Manual of Quantitative Immunoelectrophoresis. Methods and Applications', 'author': 'Axelsen N. H.', 'year': '1973'} / A Manual of Quantitative Immunoelectrophoresis. Methods and Applications by Axelsen N. H. (1973)
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10.1111/j.1432-1033.1974.tb03599.x
10.1073/pnas.80.10.3116
10.1126/science.6823544
10.1073/pnas.79.22.7036
10.1042/bj1960327 / Biochem. J. / Developmental changes in the composition of polyadenylated RNA isolated from free and membrane‐bound polyribosomes of the rat forebrain, analysed by translation in vitro by Hall C. (1981)
10.1016/0006-8993(83)91337-9
{'key': 'e_1_2_2_10_1', 'first-page': '300', 'volume-title': 'Modern Trends in Human Leukaemia', 'author': 'Hunt T.', 'year': '1974'} / Modern Trends in Human Leukaemia by Hunt T. (1974)
10.1038/194495a0
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10.1111/j.1471-4159.1974.tb04419.x
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10.1111/j.1365-3083.1982.tb00636.x
10.1083/jcb.92.1.1
10.1038/304347a0

Dates

Type	When
Created	18 years, 8 months ago (Dec. 13, 2006, 7:10 a.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 11:41 a.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 5:17 a.m.)
Issued	40 years, 5 months ago (March 1, 1985)
Published	40 years, 5 months ago (March 1, 1985)
Published Online	18 years, 8 months ago (Dec. 12, 2006)
Published Print	40 years, 5 months ago (March 1, 1985)

Funders 0

None

BibTeX

@article{Hansen_1985, title={Cell‐Free Synthesis of the D2‐Cell Adhesion Molecule: Evidence for Three Primary Translation Products}, volume={44}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1985.tb12873.x}, DOI={10.1111/j.1471-4159.1985.tb12873.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Hansen, Ole C. and Nybroe, Ole and Bock, Elisabeth}, year={1985}, month=mar, pages={712–717} }

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