DOI: 10.1111/j.1471-4159.1985.tb07200.x. Subcellular Distribution and Developmental Expression of Cholesterol Ester Hydrolases in Fetal Rat Brain Cultures

Subcellular Distribution and Developmental Expression of Cholesterol Ester Hydrolases in Fetal Rat Brain Cultures

10.1111/j.1471-4159.1985.tb07200.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Cholesterol ester hydrolase activities previously have been identified in brain and linked to the production of myelin, which has very low levels of esterified cholesterol. We have studied two cholesterol ester hydrolase activities (termed the pH 6.0 and pH 7.2 activities) in cultures derived from 19‐ to 21‐day‐old dissociated fetal rat brains and in developing rat brain. In vivo the levels of both the pH 6.0 and pH 7.2 activities began to increase by about 10 postnatal days, reached maximal levels at 20 days (20 and 1.5 nmol/h/mg protein, respectively), and thereafter remained nearly constant (pH 6.0) or decreased somewhat before becoming constant (pH 7.2). In contrast, in the cultures the pH 6.0 cholesterol ester hydrolase activity was low until 21 days in culture (DIC; 20 nmol/h/mg protein), increased to a peak activity at 31 DIC (60 nmol/h/mg protein), remained high for 24 days, and finally decreased (18 nmol/h/mg protein at 63 DIC); the pH 7.2 cholesterol ester hydrolase activity was very low until 20 DIC, increased to a peak activity at 31 days (3 nmol/h/mg protein), and thereafter decreased to a lower level (2 nmol/h/mg protein) that was maintained for about 24 days before decreasing (0.7 nmol/h/mg protein at 63 DIC). Therefore, (a) the time courses of appearance of both cholesterol ester hydrolase activities were delayed by 10–14 days relative to that seen in vivo, and (b) the specific activities observed in the cultures were transiently two‐ to three‐fold higher than in rat brain, but then declined to levels characteristic of whole brain homogenates. Subcellular fractionation of the cultures demonstrated that the pH 7.2 cholesterol ester hydrolase activity, along with myelin basic protein and 2′,3′‐cyclic nucleotide‐3′‐phosphohydrolase activity, was enriched in a membrane fraction collected at an interface between 0.32 M and 0.9 M sucrose; the pH 6.0 cholesterol ester hydrolase activity, in contrast, was enriched in the microsomal fraction.

Bibliography

Bhat, S., & Pfeiffer, S. E. (1985). Subcellular Distribution and Developmental Expression of Cholesterol Ester Hydrolases in Fetal Rat Brain Cultures. Journal of Neurochemistry, 45(5), 1356â1362. Portico.

Authors 2

Shama Bhat (first)
S. E. Pfeiffer (additional)

References 39 Referenced 8

10.1111/j.1471-4159.1959.tb13207.x
10.1002/jnr.490140103
10.1073/pnas.78.3.1953
10.1073/pnas.74.8.3360
{'key': 'e_1_2_2_6_1', 'first-page': '252', 'article-title': 'Cholesterol ester hydrolases in primary cultures of fetal rat brain', 'volume': '12', 'author': 'Bhat S.', 'year': '1981', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Cholesterol ester hydrolases in primary cultures of fetal rat brain by Bhat S. (1981)
10.1073/pnas.78.2.1283
10.1111/j.1471-4159.1978.tb00123.x
10.1016/0003-9861(79)90281-9
10.1111/j.1471-4159.1970.tb02240.x
10.1016/B978-1-4831-9939-9.50011-5
10.1016/0005-2760(71)90175-5
10.1111/j.1471-4159.1972.tb01259.x
10.1016/S0021-9258(19)44176-8 / J. Biol. Chem. / Cholesterol ester metabolism in rat brain by Eto Y. (1973)
10.1111/j.1471-4159.1973.tb00260.x
10.1016/0014-4886(73)90193-3
10.1016/0076-6879(74)32015-0
10.1016/0014-4886(74)90160-5
10.1111/j.1471-4159.1976.tb05147.x
10.1111/j.1471-4159.1977.tb10620.x
10.1111/j.1471-4159.1981.tb00572.x
10.1111/j.1471-4159.1978.tb00125.x
10.1016/S0021-9258(19)52451-6
10.1083/jcb.85.3.890
10.1111/j.1471-4159.1973.tb07519.x
10.1097/00005072-196610000-00006
10.1007/978-1-4757-6066-8_7
10.1002/jnr.490060312
10.1042/bj1320381 / Biochem. J. / Regulation by cytidine nucleotides of the acylation of sn‐[14C]glycerol‐3‐phosphate by Possmayer F. (1973)
10.1111/j.1471-4159.1979.tb04573.x
10.1002/jnr.490100208
{'key': 'e_1_2_2_32_1', 'volume-title': 'Statistics for the Biological Sciences', 'author': 'Schefler W. C.', 'year': '1969'} / Statistics for the Biological Sciences by Schefler W. C. (1969)
10.1016/0022-1759(72)90005-1
10.1111/j.1471-4159.1985.tb07202.x
10.1111/j.1471-4159.1976.tb02612.x
10.1083/jcb.32.2.415
10.1097/00005072-197007000-00005
10.1111/j.1471-4159.1964.tb06735.x
10.1111/j.1471-4159.1980.tb06294.x
{'key': 'e_1_2_2_40_1', 'first-page': '1', 'volume-title': 'Methods in Neurochemistry, Vol 2', 'author': 'Whittaker V. P.', 'year': '1972'} / Methods in Neurochemistry, Vol 2 by Whittaker V. P. (1972)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 2:42 p.m.)
Indexed	1 year, 10 months ago (Oct. 21, 2023, 1:24 a.m.)
Issued	39 years, 9 months ago (Nov. 1, 1985)
Published	39 years, 9 months ago (Nov. 1, 1985)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	39 years, 9 months ago (Nov. 1, 1985)

Funders 0

None

BibTeX

@article{Bhat_1985, title={Subcellular Distribution and Developmental Expression of Cholesterol Ester Hydrolases in Fetal Rat Brain Cultures}, volume={45}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1985.tb07200.x}, DOI={10.1111/j.1471-4159.1985.tb07200.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Bhat, Shama and Pfeiffer, S. E.}, year={1985}, month=nov, pages={1356–1362} }

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