DOI: 10.1111/j.1471-4159.1985.tb05525.x. Abnormal Expression of the Myelin‐Associated Glycoprotein in the Central Nervous System of Dysmyelinating Mutant Mice

Abnormal Expression of the Myelin‐Associated Glycoprotein in the Central Nervous System of Dysmyelinating Mutant Mice

10.1111/j.1471-4159.1985.tb05525.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Total cytoplasmic brain RNA was isolated at two different ages from three neurological mutant mice (qk/qk, jp/Y, and shi/shi) and their apparently normal littermates. This RNA was translated in vitro in a rabbit reticulocyte lysate system. Myelin‐associated glycoprotein (MAG)‐related polypeptides were immunoprecipitated from equal amounts of total translation products derived from mRNA of mutant animals, normal litter mates, or control animals. The developmentally regulated synthesis of MAG polypeptides was compared among the mutants and normal animals. mRNA from qk/qk brains synthesized an overabundance of p67MAG (five‐ to sevenfold) which may be compensation for a decreased synthesis of p72MAG. mRNA from jp/Y brains synthesized less than 10% of normal amounts of both MAG polypep tides. The quantity of MAG synthesized by 15‐day shi/shi brain mRNA was slightly decreased compared with normal brain mRNA but the quantity of MAG synthe sized by adult shi/shi brain mRNA was normal. No apparent differences were detected in the sizes of the MAG polypeptides synthesized by any of the mutants studied. The data suggest that the genetic defect in qk/qk mutants directly or indirectly affects the coordinated develop mental regulation of MAG polypeptide synthesis leading to an overabundance of the MAG polypeptide that is normally found in older animals. The jp/Y mutation appears to affect general myelin protein synthesis. Finally, shi/shi mutants may have a delayed synthesis of MAG. The data are discussed in the light of recent observations concerning the synthesis of myelin proteins and their proposed role in myelin assembly.

Bibliography

Frail, D. E., & Braun, P. E. (1985). Abnormal Expression of the MyelinâAssociated Glycoprotein in the Central Nervous System of Dysmyelinating Mutant Mice. Journal of Neurochemistry, 45(4), 1071â1075. Portico.

Authors 2

Donald E. Frail (first)
Peter E. Braun (additional)

References 28 Referenced 47

10.1111/j.1471-4159.1983.tb08142.x
10.1111/j.1471-4159.1978.tb12479.x
10.1111/j.1471-4159.1980.tb06287.x
10.1111/j.1471-4159.1984.tb02744.x
{'key': 'e_1_2_2_6_1', 'first-page': '256', 'article-title': 'Myelin basic protein gene expression in normal, quaking, jimpy, and msd mice', 'volume': '13', 'author': 'Carnow T. B.', 'year': '1982', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Myelin basic protein gene expression in normal, quaking, jimpy, and msd mice by Carnow T. B. (1982)
{'key': 'e_1_2_2_7_1', 'first-page': '207', 'article-title': 'Synthesis and degradation of myelin basic protein in normal and jimpy mouse brain', 'volume': '6', 'author': 'Carson J. H.', 'year': '1975', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Synthesis and degradation of myelin basic protein in normal and jimpy mouse brain by Carson J. H. (1975)
10.1016/0304-3940(79)91490-3
10.1016/S0021-9258(17)42683-4 / J. Biol. Chem. / Two developmentally regulated messenger RNAs differing in their coding region may exist for the myelin‐associated glycoprotein by Frail D. E. (1984)
10.1007/978-1-4757-1830-0_14
{'key': 'e_1_2_2_11_1', 'first-page': '213', 'article-title': 'Myelin‐associated glycoprotein in the CNS and PNS of developing rodents', 'volume': '13', 'author': 'Johnson D.', 'year': '1982', 'journal-title': 'Trans. Am. Soc. Neurochem.'} / Trans. Am. Soc. Neurochem. / Myelin‐associated glycoprotein in the CNS and PNS of developing rodents by Johnson D. (1982)
10.1111/j.1471-4159.1974.tb12193.x
10.1016/0006-8993(78)90282-2
10.1111/j.1471-4159.1974.tb06054.x
10.1111/j.1471-4159.1974.tb11592.x
10.1016/0304-4165(75)90176-2
10.1111/j.1471-4159.1978.tb10785.x
10.1016/0165-3806(84)90086-5
10.1097/00005072-197407000-00001
{'key': 'e_1_2_2_20_1', 'first-page': '545', 'volume-title': 'Neurological Mutations Affecting Myelination', 'author': 'Norton W. T.', 'year': '1980'} / Neurological Mutations Affecting Myelination by Norton W. T. (1980)
PereyraP. M.(1983)Studies on the mechanism of assembly of myelin in the central nervous system.Ph.D. thesis McGill University Montreal .
10.1111/j.1471-4159.1983.tb09041.x
10.1159/000112356
10.1016/0092-8674(83)90536-6
10.1097/00005072-197010000-00001
10.1083/jcb.72.3.726
10.1126/science.144.3616.309
10.1097/00005072-197104000-00009
10.1016/0006-8993(71)90417-3

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:25 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 5:39 p.m.)
Indexed	4 months, 2 weeks ago (April 10, 2025, 6:30 a.m.)
Issued	39 years, 10 months ago (Oct. 1, 1985)
Published	39 years, 10 months ago (Oct. 1, 1985)
Published Online	18 years, 10 months ago (Oct. 5, 2006)
Published Print	39 years, 10 months ago (Oct. 1, 1985)

Funders 0

None

BibTeX

@article{Frail_1985, title={Abnormal Expression of the Myelin‐Associated Glycoprotein in the Central Nervous System of Dysmyelinating Mutant Mice}, volume={45}, ISSN={1471-4159}, url={http://dx.doi.org/10.1111/j.1471-4159.1985.tb05525.x}, DOI={10.1111/j.1471-4159.1985.tb05525.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Frail, Donald E. and Braun, Peter E.}, year={1985}, month=oct, pages={1071–1075} }

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